Gene description for PFDN2
Gene name prefoldin subunit 2
Gene symbol PFDN2
Other names/aliases PFD2
Species Homo sapiens
 Database cross references - PFDN2
ExoCarta ExoCarta_5202
Entrez Gene 5202
HGNC 8867
MIM 613466
UniProt Q9UHV9  
 PFDN2 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Prostate cancer cells 25844599    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for PFDN2
Molecular Function
    protein binding GO:0005515 IPI
    unfolded protein binding GO:0051082 NAS
    protein binding involved in protein folding GO:0044183 IPI
Biological Process
    protein folding GO:0006457 TAS
    positive regulation of cytoskeleton organization GO:0051495 IDA
    'de novo' posttranslational protein folding GO:0051084 TAS
    cellular protein metabolic process GO:0044267 TAS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    nucleolus GO:0005730 IDA
    prefoldin complex GO:0016272 NAS
    mitochondrion GO:0005739 IDA
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified PFDN2 in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 275
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|RAB5A
EV Cytosolic markers
CD9|CD82|CD63|CD81
EV Membrane markers
AIF
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
3
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PFDN2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNRNP40 9410
Affinity Capture-MS Homo sapiens
2 VBP1 7411
Co-purification Homo sapiens
3 WDR8  
Affinity Capture-MS Homo sapiens
4 BRDG1  
Affinity Capture-MS Homo sapiens
5 MAP3K3  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PFDN2 is involved
PathwayEvidenceSource
Prefoldin mediated transfer of substrate to CCT/TriC IEA Reactome





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