Gene description for PRKAB1
Gene name protein kinase, AMP-activated, beta 1 non-catalytic subunit
Gene symbol PRKAB1
Other names/aliases AMPK
HAMPKb
Species Homo sapiens
 Database cross references - PRKAB1
ExoCarta ExoCarta_5564
Vesiclepedia VP_5564
Entrez Gene 5564
HGNC 9378
MIM 602740
UniProt Q9Y478  
 PRKAB1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PRKAB1
Molecular Function
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IBA
Biological Process
    fatty acid biosynthetic process GO:0006633 IEA
    signal transduction GO:0007165 IBA
    cellular response to nutrient levels GO:0031669 IDA
    cellular response to nutrient levels GO:0031669 ISS
    cellular response to nutrient levels GO:0031669 NAS
    nail development GO:0035878 IEA
    positive regulation of cold-induced thermogenesis GO:0120162 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    nucleotide-activated protein kinase complex GO:0031588 IBA
    nucleotide-activated protein kinase complex GO:0031588 IPI
    nucleotide-activated protein kinase complex GO:0031588 ISS
 Experiment description of studies that identified PRKAB1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PRKAB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 F10 2159
Two-hybrid Homo sapiens
2 HEATR6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 ATP6V1B2 526
Co-fractionation Homo sapiens
4 NSF 4905
Affinity Capture-MS Homo sapiens
5 PRKAA1 5562
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 MINK1 50488
Affinity Capture-MS Homo sapiens
7 FNIP1  
Affinity Capture-Western Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 DHX36 170506
Affinity Capture-MS Homo sapiens
10 SLC31A1 1317
Biochemical Activity Homo sapiens
11 CCKBR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 GRB2 2885
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
13 CUTA 51596
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ATP6V1A 523
Co-fractionation Homo sapiens
15 LAMTOR1 55004
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 DDX58 23586
Affinity Capture-RNA Homo sapiens
17 Prkaa1  
Affinity Capture-MS Mus musculus
18 CBR1 873
Affinity Capture-MS Homo sapiens
19 TGM3 7053
Affinity Capture-MS Homo sapiens
20 GYS1 2997
Affinity Capture-MS Homo sapiens
21 MCL1 4170
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 MID1IP1  
Affinity Capture-MS Homo sapiens
23 RARRES3  
Affinity Capture-MS Homo sapiens
24 AP1M1 8907
Co-fractionation Homo sapiens
25 AP1G1 164
Co-fractionation Homo sapiens
26 PRKAA2 5563
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
27 DSC1 1823
Affinity Capture-MS Homo sapiens
28 FKBP4 2288
Co-fractionation Homo sapiens
29 HARS2 23438
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 DCLRE1C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 PHKG2 5261
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 PRKAG2 51422
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 MAP3K7  
Biochemical Activity Homo sapiens
34 TUBB1 81027
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 MARS 4141
Affinity Capture-MS Homo sapiens
36 APP 351
Reconstituted Complex Homo sapiens
37 PRKAG1 5571
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
38 PEX1 5189
Affinity Capture-MS Homo sapiens
39 POLR3K  
Affinity Capture-MS Homo sapiens
40 TUBA4A 7277
Affinity Capture-MS Homo sapiens
41 SNRNP200 23020
Affinity Capture-MS Homo sapiens
42 ATXN7L1 222255
Affinity Capture-MS Homo sapiens
43 MAPK6  
Two-hybrid Homo sapiens
44 C12orf49  
Positive Genetic Homo sapiens
45 PFDN2 5202
Co-fractionation Homo sapiens
46 RFESD  
Affinity Capture-MS Homo sapiens
47 SREBF2 6721
Positive Genetic Homo sapiens
48 SLC9A5  
Affinity Capture-MS Homo sapiens
49 ACACA 31
Biochemical Activity Homo sapiens
50 TRIM11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 NDUFA7 4701
Two-hybrid Homo sapiens
52 STIM2 57620
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 TRMT44  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 PLA2G2D  
Affinity Capture-MS Homo sapiens
55 AP1B1 162
Co-fractionation Homo sapiens
56 ULK1  
Affinity Capture-Western Homo sapiens
57 SCRIB 23513
Affinity Capture-MS Homo sapiens
58 NEK4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 PRKAG3  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 MYH10 4628
Affinity Capture-MS Homo sapiens
61 CASP14 23581
Affinity Capture-MS Homo sapiens
62 PRKAB2 5565
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 MAST3  
Affinity Capture-MS Homo sapiens
64 CIDEA  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
65 AP5Z1 9907
Affinity Capture-MS Homo sapiens
66 EZR 7430
Affinity Capture-MS Homo sapiens
67 SOGA1 140710
Affinity Capture-Western Homo sapiens
68 HDAC5 10014
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
69 CAMKK2 10645
Affinity Capture-MS Homo sapiens
70 ACLY 47
Affinity Capture-MS Homo sapiens
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