Gene description for PRKAG2
Gene name protein kinase, AMP-activated, gamma 2 non-catalytic subunit
Gene symbol PRKAG2
Other names/aliases AAKG
AAKG2
CMH6
H91620p
WPWS
Species Homo sapiens
 Database cross references - PRKAG2
ExoCarta ExoCarta_51422
Vesiclepedia VP_51422
Entrez Gene 51422
HGNC 9386
MIM 602743
UniProt Q9UGJ0  
 PRKAG2 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for PRKAG2
Molecular Function
    AMP-activated protein kinase activity GO:0004679 IEA
    cAMP-dependent protein kinase inhibitor activity GO:0004862 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    cAMP-dependent protein kinase regulator activity GO:0008603 IMP
    phosphorylase kinase regulator activity GO:0008607 IMP
    AMP binding GO:0016208 IBA
    protein kinase regulator activity GO:0019887 IBA
    protein kinase regulator activity GO:0019887 IDA
    protein kinase binding GO:0019901 IBA
    protein kinase binding GO:0019901 IDA
    protein kinase activator activity GO:0030295 IMP
    ADP binding GO:0043531 IDA
Biological Process
    glycogen metabolic process GO:0005977 IMP
    regulation of glycolytic process GO:0006110 IBA
    regulation of glycolytic process GO:0006110 IMP
    negative regulation of protein kinase activity GO:0006469 IDA
    fatty acid biosynthetic process GO:0006633 IEA
    ATP biosynthetic process GO:0006754 TAS
    positive regulation of peptidyl-threonine phosphorylation GO:0010800 IMP
    sterol biosynthetic process GO:0016126 TAS
    regulation of fatty acid metabolic process GO:0019217 IMP
    cellular response to nutrient levels GO:0031669 ISS
    intracellular signal transduction GO:0035556 IMP
    cellular response to glucose starvation GO:0042149 IBA
    regulation of carbon utilization GO:0043609 IBA
    positive regulation of gluconeogenesis GO:0045722 IBA
    positive regulation of protein kinase activity GO:0045860 IMP
    regulation of fatty acid oxidation GO:0046320 TAS
    regulation of D-glucose import GO:0046324 TAS
Subcellular Localization
    extracellular space GO:0005615 HDA
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    nucleotide-activated protein kinase complex GO:0031588 IBA
    nucleotide-activated protein kinase complex GO:0031588 IDA
    nucleotide-activated protein kinase complex GO:0031588 ISS
 Experiment description of studies that identified PRKAG2 in exosomes
1
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PRKAG2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF3A 8661
Affinity Capture-MS Homo sapiens
2 PRKAA1 5562
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 NEFH 4744
Affinity Capture-MS Homo sapiens
4 SLC31A1 1317
Biochemical Activity Homo sapiens
5 GEMIN2 8487
Affinity Capture-MS Homo sapiens
6 ALOXE3  
Affinity Capture-MS Homo sapiens
7 ASNS 440
Affinity Capture-MS Homo sapiens
8 NF2 4771
Affinity Capture-MS Homo sapiens
9 PRKAA2 5563
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
11 DCLRE1C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 PHKG2 5261
Affinity Capture-MS Homo sapiens
13 MAP1B 4131
Affinity Capture-MS Homo sapiens
14 FKBP4 2288
Affinity Capture-MS Homo sapiens
15 TARS 6897
Affinity Capture-MS Homo sapiens
16 PRKAG1 5571
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 MAP1LC3A 84557
Affinity Capture-MS Homo sapiens
18 PGK1 5230
Affinity Capture-MS Homo sapiens
19 STIM2 57620
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 WDR83  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 SCRIB 23513
Affinity Capture-MS Homo sapiens
22 GANAB 23193
Affinity Capture-MS Homo sapiens
23 MAP1A 4130
Affinity Capture-MS Homo sapiens
24 PRKAB2 5565
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 GMPS 8833
Affinity Capture-MS Homo sapiens
26 PRKAB1 5564
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 CAMK2B 816
Affinity Capture-MS Homo sapiens
28 PFAS 5198
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PRKAG2 is involved
PathwayEvidenceSource
Activation of AMPK downstream of NMDARs IEA Reactome
Activation of NMDA receptors and postsynaptic events IEA Reactome
Activation of PPARGC1A (PGC-1alpha) by phosphorylation IEA Reactome
AMPK inhibits chREBP transcriptional activation activity TAS Reactome
AMPK inhibits chREBP transcriptional activation activity IEA Reactome
Autophagy IEA Reactome
Autophagy TAS Reactome
Carnitine shuttle TAS Reactome
Energy dependent regulation of mTOR by LKB1-AMPK TAS Reactome
Fatty acid metabolism TAS Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
Integration of energy metabolism TAS Reactome
Integration of energy metabolism IEA Reactome
Lipophagy IEA Reactome
Macroautophagy IEA Reactome
Macroautophagy TAS Reactome
Membrane Trafficking IEA Reactome
Metabolism TAS Reactome
Metabolism IEA Reactome
Metabolism of lipids TAS Reactome
Mitochondrial biogenesis IEA Reactome
MTOR signalling TAS Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Organelle biogenesis and maintenance IEA Reactome
Post NMDA receptor activation events IEA Reactome
Regulation of TP53 Activity TAS Reactome
Regulation of TP53 Activity through Phosphorylation TAS Reactome
RNA Polymerase II Transcription TAS Reactome
Selective autophagy IEA Reactome
Signal Transduction TAS Reactome
TP53 Regulates Metabolic Genes TAS Reactome
Transcriptional Regulation by TP53 TAS Reactome
Translocation of SLC2A4 (GLUT4) to the plasma membrane IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Vesicle-mediated transport IEA Reactome





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