Gene description for PRKAG2
Gene name protein kinase, AMP-activated, gamma 2 non-catalytic subunit
Gene symbol PRKAG2
Other names/aliases AAKG
AAKG2
CMH6
H91620p
WPWS
Species Homo sapiens
 Database cross references - PRKAG2
ExoCarta ExoCarta_51422
Entrez Gene 51422
HGNC 9386
MIM 602743
UniProt Q9UGJ0  
 PRKAG2 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for PRKAG2
Molecular Function
    protein kinase binding GO:0019901 IDA
    cAMP-dependent protein kinase regulator activity GO:0008603 IMP
    ADP binding GO:0043531 IDA
    phosphorylase kinase regulator activity GO:0008607 IMP
    protein kinase activator activity GO:0030295 IMP
    AMP-activated protein kinase activity GO:0004679 IEA
    ATP binding GO:0005524 IDA
    AMP binding GO:0016208 IEA
    cAMP-dependent protein kinase inhibitor activity GO:0004862 IDA
Biological Process
    sterol biosynthetic process GO:0016126 TAS
    gene expression GO:0010467 TAS
    mitochondrion organization GO:0007005 TAS
    energy reserve metabolic process GO:0006112 TAS
    regulation of fatty acid biosynthetic process GO:0042304 TAS
    insulin receptor signaling pathway GO:0008286 TAS
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    regulation of fatty acid oxidation GO:0046320 TAS
    regulation of glycolytic process GO:0006110 IMP
    glycogen metabolic process GO:0005977 IMP
    negative regulation of protein serine/threonine kinase activity GO:0071901 IDA
    regulation of fatty acid metabolic process GO:0019217 IMP
    regulation of glucose import GO:0046324 TAS
    fatty acid biosynthetic process GO:0006633 IEA
    intracellular signal transduction GO:0035556 IMP
    positive regulation of protein kinase activity GO:0045860 IMP
    carnitine shuttle GO:0006853 TAS
    negative regulation of protein kinase activity GO:0006469 IDA
    cell cycle arrest GO:0007050 TAS
    protein phosphorylation GO:0006468 IEA
    cellular lipid metabolic process GO:0044255 TAS
    ATP biosynthetic process GO:0006754 TAS
    small molecule metabolic process GO:0044281 TAS
    membrane organization GO:0061024 TAS
    organelle organization GO:0006996 TAS
    positive regulation of peptidyl-threonine phosphorylation GO:0010800 IMP
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 TAS
    AMP-activated protein kinase complex GO:0031588 IDA
    extracellular space GO:0005615 IDA
 Experiment description of studies that identified PRKAG2 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PRKAG2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRKAG1 5571
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
2 PRKAB1  
Two-hybrid Homo sapiens
3 PRKAB2 5565
Two-hybrid Homo sapiens
View the network image/svg+xml



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