Gene description for NEFH
Gene name
neurofilament, heavy polypeptide
Gene symbol
NEFH
Other names/aliases
NFH
Species
Homo sapiens
Database cross references - NEFH
ExoCarta
ExoCarta_4744
Entrez Gene
4744
HGNC
7737
MIM
162230
UniProt
P12036
NEFH identified in exosomes derived from the following tissue/cell type
Liver cancer cells
25265333
Mesenchymal stem cells
Unpublished / Not applicable
Gene ontology annotations for NEFH
Experiment description of studies that identified NEFH in exosomes
1
Experiment ID
267
ISEV standards
✘
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✘
EV Membrane markers
✔
CANX|HSP90B1
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25265333
Organism
Homo sapiens
Experiment description
Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh‑7 Cells Using SILAC Labeling and LC−MS/MS
Authors
Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J
Journal name
J Proteome Res
Publication year
2014
Sample
Liver cancer cells
Sample name
Huh-7 - Transfected with HBx plasmids
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
2
Experiment ID
126
ISEV standards
✘
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [LTQ-FT Ultra]
PubMed ID
Unpublished / Not applicable
Organism
Homo sapiens
Experiment description
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors
Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name
Mesenchymal Stem Cell Therapy
Publication year
2011
Sample
Mesenchymal stem cells
Sample name
huES9.E1
Isolation/purification methods
HPLC
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Antobody array Mass spectrometry
Protein-protein interactions for NEFH
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
PKN1
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
2
NEFL
4747
Co-purification
Homo sapiens
3
NEFM
4741
Invitro
Homo sapiens
View the network
image/svg+xml
Pathways in which NEFH is involved
No pathways found