Experiment description of studies that identified NEFH in exosomes

1 Experiment ID 267 MISEV standards ✘ Biophysical techniques ✔ TSG101|Alix Enriched markers ✔ CANX|HSP90B1 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25265333     Organism Homo sapiens Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J" Journal name J Proteome Res Publication year 2014 Sample Liver cancer cells Sample name Huh-7 - Transfected with HBx plasmids Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 126 MISEV standards ✘ Biophysical techniques ✔ GAPDH Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [LTQ-FT Ultra] PubMed ID Unpublished / Not applicable Organism Homo sapiens Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim" Journal name Mesenchymal Stem Cell Therapy Publication year 2011 Sample Mesenchymal stem cells Sample name huES9.E1 Isolation/purification methods HPLC Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Antobody array Mass spectrometry

3 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for NEFH

Protein Interactor ExoCarta ID Identification method PubMed Species 1 DUSP7   Affinity Capture-MS Homo sapiens 2 PRSS16 10279 Affinity Capture-MS Homo sapiens 3 GAN 8139 Affinity Capture-MS Homo sapiens 4 FADD 8772 Affinity Capture-MS Homo sapiens 5 HSD17B6   Affinity Capture-MS Homo sapiens 6 C18orf21   Affinity Capture-MS Homo sapiens 7 GRB2 2885 Affinity Capture-MS Homo sapiens 8 WDR77 79084 Affinity Capture-MS Homo sapiens 9 CDC73   Affinity Capture-MS Homo sapiens 10 TARBP1 6894 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 11 FCF1   Affinity Capture-MS Homo sapiens 12 C16orf70   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 13 ASAH1 427 Affinity Capture-MS Homo sapiens 14 PRKAG2 51422 Affinity Capture-MS Homo sapiens 15 DFFB   Affinity Capture-MS Homo sapiens 16 CUL1 8454 Affinity Capture-MS Homo sapiens 17 CERS3   Affinity Capture-MS Homo sapiens 18 PI4KA 5297 Affinity Capture-MS Homo sapiens 19 RFFL 117584 Affinity Capture-MS Homo sapiens 20 MBNL1 4154 Affinity Capture-MS Homo sapiens 21 GPATCH2L   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 22 SSUH2   Affinity Capture-MS Homo sapiens 23 INA 9118 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 24 PI4KAP1   Affinity Capture-MS Homo sapiens 25 CCDC51 79714 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 26 NEFL 4747 Co-purification Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 27 NEFM 4741 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 28 GOT1 2805 Affinity Capture-MS Homo sapiens 29 GLYR1 84656 Affinity Capture-MS Homo sapiens 30 PKN1 5585 Two-hybrid Homo sapiens Reconstituted Complex Homo sapiens 31 MT1M 4499 Affinity Capture-MS Homo sapiens View the network

Pathways in which NEFH is involved

No pathways found