Gene description for RFFL
Gene name ring finger and FYVE-like domain containing E3 ubiquitin protein ligase
Gene symbol RFFL
Other names/aliases CARP-2
CARP2
FRING
RIFIFYLIN
RNF189
RNF34L
Species Homo sapiens
 Database cross references - RFFL
ExoCarta ExoCarta_117584
Entrez Gene 117584
HGNC 24821
MIM 609735
UniProt Q8WZ73  
 RFFL identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for RFFL
Molecular Function
    ligase activity GO:0016874 IEA
    p53 binding GO:0002039 IPI
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    protease binding GO:0002020 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    ubiquitin protein ligase activity GO:0061630 IDA
    protein kinase binding GO:0019901 IPI
Biological Process
    negative regulation of tumor necrosis factor-mediated signaling pathway GO:0010804 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IMP
    regulation of fibroblast migration GO:0010762 ISS
    regulation of TOR signaling GO:0032006 IC
    ubiquitin-dependent protein catabolic process GO:0006511 IMP
    negative regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902042 IMP
    intracellular protein transport GO:0006886 IEA
    negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis GO:2001271 IMP
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IMP
    negative regulation of signal transduction by p53 class mediator GO:1901797 IMP
    protein K48-linked ubiquitination GO:0070936 IDA
Subcellular Localization
    tumor necrosis factor receptor superfamily complex GO:0002947 IDA
    lysosome GO:0005764 IDA
    endosome membrane GO:0010008 IDA
    plasma membrane GO:0005886 IDA
    cytoplasm GO:0005737 IDA
    recycling endosome membrane GO:0055038 IEA
    Golgi membrane GO:0000139 IDA
    cytoplasmic membrane-bounded vesicle GO:0016023 IEA
    membrane GO:0016020 IDA
    cytosol GO:0005829 IEA
 Experiment description of studies that identified RFFL in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RFFL
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which RFFL is involved
No pathways found





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