Gene description for RFFL
Gene name ring finger and FYVE-like domain containing E3 ubiquitin protein ligase
Gene symbol RFFL
Other names/aliases CARP-2
CARP2
FRING
RIFIFYLIN
RNF189
RNF34L
Species Homo sapiens
 Database cross references - RFFL
ExoCarta ExoCarta_117584
Vesiclepedia VP_117584
Entrez Gene 117584
HGNC 24821
MIM 609735
UniProt Q8WZ73  
 RFFL identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for RFFL
Molecular Function
    protease binding GO:0002020 IPI
    p53 binding GO:0002039 IPI
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    metal ion binding GO:0046872 IEA
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 IDA
    ubiquitin protein ligase activity GO:0061630 TAS
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    ubiquitin-dependent protein catabolic process GO:0006511 IMP
    apoptotic process GO:0006915 IEA
    regulation of fibroblast migration GO:0010762 ISS
    negative regulation of tumor necrosis factor-mediated signaling pathway GO:0010804 IMP
    regulation of TOR signaling GO:0032006 IC
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IMP
    protein K48-linked ubiquitination GO:0070936 IDA
    regulation of signal transduction by p53 class mediator GO:1901796 TAS
    negative regulation of signal transduction by p53 class mediator GO:1901797 IMP
    negative regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902042 IBA
    negative regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902042 IMP
    negative regulation of cysteine-type endopeptidase activity GO:2000117 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    lysosome GO:0005764 IDA
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    endosome membrane GO:0010008 IDA
    membrane GO:0016020 HDA
    recycling endosome membrane GO:0055038 IEA
 Experiment description of studies that identified RFFL in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RFFL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STUB1 10273
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
2 ESPL1  
Biochemical Activity Homo sapiens
3 PRKD2 25865
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 KCNH2  
Affinity Capture-Western Homo sapiens
5 TUSC2  
Affinity Capture-MS Homo sapiens
6 EFCAB7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 NEFH 4744
Affinity Capture-MS Homo sapiens
8 USP7 7874
Biochemical Activity Homo sapiens
9 RNF34  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 SHC1 6464
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 HSPA6 3310
Affinity Capture-MS Homo sapiens
12 TP53 7157
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 IQCE 23288
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 MAPK1 5594
Affinity Capture-MS Homo sapiens
15 USP5 8078
Biochemical Activity Homo sapiens
16 ATXN3L  
Biochemical Activity Homo sapiens
17 MAPK3 5595
Affinity Capture-MS Homo sapiens
18 CUL3 8452
Affinity Capture-MS Homo sapiens
19 PARK2  
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
20 OTUB1 55611
Biochemical Activity Homo sapiens
21 RIPK1 8737
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
22 RFFL 117584
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
23 UBE2G2  
Two-hybrid Homo sapiens
24 UBE2D2 7322
Reconstituted Complex Homo sapiens
25 TUBB2B 347733
Affinity Capture-MS Homo sapiens
26 UCHL5 51377
Biochemical Activity Homo sapiens
27 USP28 57646
Biochemical Activity Homo sapiens
28 VCP 7415
Affinity Capture-Western Homo sapiens
29 RAB7A 7879
Affinity Capture-Western Homo sapiens
30 UQCRC1 7384
Affinity Capture-Western Homo sapiens
31 UBE2D3 7323
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
32 OTUB2  
Biochemical Activity Homo sapiens
33 ATXN3 4287
Biochemical Activity Homo sapiens
34 UBE2D1 7321
Reconstituted Complex Homo sapiens
35 MDM2  
Affinity Capture-Western Homo sapiens
36 STAM 8027
Biochemical Activity Homo sapiens
37 HSPA2 3306
Affinity Capture-MS Homo sapiens
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