Gene description for PKN1
Gene name protein kinase N1
Gene symbol PKN1
Other names/aliases DBK
PAK-1
PAK1
PKN
PKN-ALPHA
PRK1
PRKCL1
Species Homo sapiens
 Database cross references - PKN1
ExoCarta ExoCarta_5585
Vesiclepedia VP_5585
Entrez Gene 5585
HGNC 9405
MIM 601032
UniProt Q16512  
 PKN1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PKN1
Molecular Function
    chromatin binding GO:0003682 IDA
    protein kinase activity GO:0004672 TAS
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 TAS
    diacylglycerol-dependent serine/threonine kinase activity GO:0004697 EXP
    diacylglycerol-dependent serine/threonine kinase activity GO:0004697 TAS
    calcium,diacylglycerol-dependent serine/threonine kinase activity GO:0004698 IEA
    diacylglycerol-dependent, calcium-independent serine/threonine kinase activity GO:0004699 IEA
    protein kinase C binding GO:0005080 IPI
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    nuclear receptor coactivator activity GO:0030374 IDA
    nuclear receptor coactivator activity GO:0030374 IMP
    small GTPase binding GO:0031267 IDA
    histone H3T11 kinase activity GO:0035402 IDA
    histone binding GO:0042393 IDA
    histone deacetylase binding GO:0042826 IDA
    nuclear androgen receptor binding GO:0050681 IDA
    protein serine kinase activity GO:0106310 IEA
Biological Process
    B cell homeostasis GO:0001782 IEA
    B cell apoptotic process GO:0001783 IEA
    regulation of germinal center formation GO:0002634 IEA
    regulation of immunoglobulin production GO:0002637 IEA
    renal system process GO:0003014 IEA
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IDA
    regulation of transcription by RNA polymerase II GO:0006357 IMP
    protein phosphorylation GO:0006468 IDA
    hyperosmotic response GO:0006972 IEA
    signal transduction GO:0007165 TAS
    epithelial cell migration GO:0010631 IMP
    negative regulation of B cell proliferation GO:0030889 IEA
    intracellular signal transduction GO:0035556 IBA
    post-translational protein modification GO:0043687 IDA
    positive regulation of DNA-templated transcription GO:0045893 IEA
    spleen development GO:0048536 IEA
    regulation of androgen receptor signaling pathway GO:0060765 TAS
    regulation of cell motility GO:2000145 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    endosome GO:0005768 IDA
    cytosol GO:0005829 TAS
    midbody GO:0030496 IDA
    cleavage furrow GO:0032154 IDA
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified PKN1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PKN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZNF282  
Two-hybrid Homo sapiens
2 HOMER3 9454
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 KRT37 8688
Two-hybrid Homo sapiens
4 CCDC103  
Two-hybrid Homo sapiens
5 NUP85 79902
Affinity Capture-MS Homo sapiens
6 ABCF1 23
Cross-Linking-MS (XL-MS) Homo sapiens
7 FUOM  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SLIRP 81892
Affinity Capture-MS Homo sapiens
9 MID1 4281
Two-hybrid Homo sapiens
10 NEUROD2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 STX11 8676
Two-hybrid Homo sapiens
12 RB1CC1 9821
Affinity Capture-MS Homo sapiens
13 SYCE3  
Two-hybrid Homo sapiens
14 CEP70  
Two-hybrid Homo sapiens
15 CEP57L1  
Two-hybrid Homo sapiens
16 CASP3 836
Biochemical Activity Homo sapiens
17 RAB3IP  
Two-hybrid Homo sapiens
18 SMYD3 64754
Two-hybrid Homo sapiens
19 CCDC138  
Two-hybrid Homo sapiens
20 MTUS2 23281
Two-hybrid Homo sapiens
21 KRT31 3881
Two-hybrid Homo sapiens
22 TOM1L1 10040
Co-fractionation Homo sapiens
23 RHOB 388
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
24 PPM1A 5494
Co-fractionation Homo sapiens
25 MAPK11  
Affinity Capture-Western Homo sapiens
26 ASH2L 9070
Co-localization Homo sapiens
27 CEP57  
Two-hybrid Homo sapiens
28 PKN2 5586
Affinity Capture-MS Homo sapiens
29 WDR5 11091
Co-localization Homo sapiens
30 TNFRSF1B  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
31 C15orf59  
Affinity Capture-MS Homo sapiens
32 GOLGA6L9  
Two-hybrid Homo sapiens
33 C1orf186  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 AEN  
Affinity Capture-MS Homo sapiens
35 PDK1 5163
Affinity Capture-Western Homo sapiens
36 RHOA 387
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
37 KRT34  
Two-hybrid Homo sapiens
38 S100A2 6273
Affinity Capture-MS Homo sapiens
39 PSMB4 5692
Two-hybrid Homo sapiens
40 MAPK12  
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
41 MAGEA10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 PARK2  
Affinity Capture-MS Homo sapiens
43 PLD1 5337
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
44 TUBA1A 7846
Affinity Capture-Western Homo sapiens
45 GOLGA2 2801
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
46 POU6F2  
Two-hybrid Homo sapiens
47 MAPT  
Biochemical Activity Homo sapiens
48 KRT16 3868
Two-hybrid Homo sapiens
49 CYSRT1 375791
Two-hybrid Homo sapiens
50 NTRK1 4914
Affinity Capture-MS Homo sapiens
51 AKAP9 10142
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
52 SPRR2D  
Two-hybrid Homo sapiens
53 DYDC1  
Two-hybrid Homo sapiens
54 PKN1 5585
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
55 HDAC5 10014
Biochemical Activity Homo sapiens
56 PPM1G 5496
Co-fractionation Homo sapiens
57 HAP1  
Two-hybrid Homo sapiens
58 KBTBD8  
Affinity Capture-Western Homo sapiens
59 SSX2IP  
Two-hybrid Homo sapiens
60 DISC1 27185
Two-hybrid Homo sapiens
61 ACTN1 87
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
62 RNF40 9810
Affinity Capture-MS Homo sapiens
63 TUBB 203068
Affinity Capture-Western Homo sapiens
64 WHAMMP3  
Affinity Capture-MS Homo sapiens
65 KMT2A  
Co-localization Homo sapiens
66 HIST2H3C 126961
Biochemical Activity Homo sapiens
67 KRT35 3886
Two-hybrid Homo sapiens
68 WBSCR22  
Two-hybrid Homo sapiens
69 PDPK1 5170
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
70 CCNT2  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
71 KRT15 3866
Two-hybrid Homo sapiens
72 COG6 57511
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 PCBP1 5093
Proximity Label-MS Homo sapiens
74 NEFL 4747
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
75 MYC  
Dosage Lethality Homo sapiens
76 RPA3 6119
Proximity Label-MS Homo sapiens
77 CCDC85B  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
78 TRIM27  
Two-hybrid Homo sapiens
79 CERS6  
Affinity Capture-MS Homo sapiens
80 USHBP1  
Two-hybrid Homo sapiens
81 RBBP5 5929
Co-localization Homo sapiens
82 CPSF3L 54973
Two-hybrid Homo sapiens
83 TSGA10  
Two-hybrid Homo sapiens
84 PA2G4 5036
Affinity Capture-MS Homo sapiens
85 HAUS1  
Two-hybrid Homo sapiens
86 EIF3F 8665
Two-hybrid Homo sapiens
87 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
88 MAP2K6 5608
Affinity Capture-Western Homo sapiens
89 TRAF2 7186
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
90 KRT36 8689
Two-hybrid Homo sapiens
91 PLAGL2  
Affinity Capture-MS Homo sapiens
92 SLC39A7 7922
Affinity Capture-MS Homo sapiens
93 BLZF1  
Two-hybrid Homo sapiens
94 SQSTM1 8878
Proximity Label-MS Homo sapiens
95 MKRN3  
Two-hybrid Homo sapiens
96 PNMA1 9240
Two-hybrid Homo sapiens
97 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
98 DDX58 23586
Affinity Capture-RNA Homo sapiens
99 KIAA1328  
Two-hybrid Homo sapiens
100 NEFH 4744
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
101 MAP2K3 5606
Affinity Capture-Western Homo sapiens
102 ADAMTSL4  
Two-hybrid Homo sapiens
103 KRT19 3880
Two-hybrid Homo sapiens
104 MAPK9 5601
Co-fractionation Homo sapiens
105 LRRC61 65999
Two-hybrid Homo sapiens
106 CDR2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
107 INHBB 3625
Affinity Capture-MS Homo sapiens
108 CCDC136  
Two-hybrid Homo sapiens
109 MIPOL1  
Two-hybrid Homo sapiens
110 HSF2BP  
Two-hybrid Homo sapiens
111 VIM 7431
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
112 PLAUR 5329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 SERTAD3  
Two-hybrid Homo sapiens
114 TRAF1 7185
Biochemical Activity Homo sapiens
View the network image/svg+xml



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