Gene description for PDK1
Gene name pyruvate dehydrogenase kinase, isozyme 1
Gene symbol PDK1
Other names/aliases -
Species Homo sapiens
 Database cross references - PDK1
ExoCarta ExoCarta_5163
Vesiclepedia VP_5163
Entrez Gene 5163
HGNC 8809
MIM 602524
UniProt Q15118  
 PDK1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PDK1
Molecular Function
    protein binding GO:0005515 IPI
    protein kinase activity GO:0004672 IDA
    ATP binding GO:0005524 IEA
    pyruvate dehydrogenase (acetyl-transferring) kinase activity GO:0004740 IMP
Biological Process
    protein phosphorylation GO:0006468 IMP
    cell proliferation GO:0008283 IMP
    glucose metabolic process GO:0006006 IEA
    intrinsic apoptotic signaling pathway in response to oxidative stress GO:0008631 IMP
    regulation of acetyl-CoA biosynthetic process from pyruvate GO:0010510 TAS
    regulation of glucose metabolic process GO:0010906 IMP
    hypoxia-inducible factor-1alpha signaling pathway GO:0097411 IMP
    small molecule metabolic process GO:0044281 TAS
    pyruvate metabolic process GO:0006090 TAS
    activation of mitophagy in response to mitochondrial depolarization GO:0098779 IMP
    cellular metabolic process GO:0044237 TAS
Subcellular Localization
    mitochondrial matrix GO:0005759 TAS
    mitochondrial pyruvate dehydrogenase complex GO:0005967 IEA
    mitochondrion GO:0005739 TAS
 Experiment description of studies that identified PDK1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PDK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Invivo Homo sapiens
Invitro Homo sapiens
2 PKN1 5585
Affinity Capture-Western Homo sapiens
3 ILK 3611
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
4 PKN2 5586
Affinity Capture-Western Homo sapiens
5 RPS6KB1 6198
Reconstituted Complex Homo sapiens
6 RPTOR 57521
Affinity Capture-Western Homo sapiens
7 DLAT 1737
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
8 PDK2 5164
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
9 RALGDS  
Affinity Capture-Western Homo sapiens
10 MTOR 2475
Phenotypic Enhancement Homo sapiens
View the network image/svg+xml
 Pathways in which PDK1 is involved
PathwayEvidenceSource
Regulation of pyruvate dehydrogenase (PDH) complex TAS Reactome
Signaling by Retinoic Acid TAS Reactome





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