Gene description for PDK1
Gene name pyruvate dehydrogenase kinase, isozyme 1
Gene symbol PDK1
Other names/aliases -
Species Homo sapiens
 Database cross references - PDK1
ExoCarta ExoCarta_5163
Vesiclepedia VP_5163
Entrez Gene 5163
HGNC 8809
MIM 602524
UniProt Q15118  
 PDK1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PDK1
Molecular Function
    protein kinase activity GO:0004672 IDA
    pyruvate dehydrogenase (acetyl-transferring) kinase activity GO:0004740 IBA
    pyruvate dehydrogenase (acetyl-transferring) kinase activity GO:0004740 IDA
    pyruvate dehydrogenase (acetyl-transferring) kinase activity GO:0004740 IMP
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
Biological Process
    glucose metabolic process GO:0006006 TAS
    cell population proliferation GO:0008283 IMP
    intrinsic apoptotic signaling pathway in response to oxidative stress GO:0008631 IMP
    regulation of acetyl-CoA biosynthetic process from pyruvate GO:0010510 IBA
    regulation of glucose metabolic process GO:0010906 IBA
    regulation of glucose metabolic process GO:0010906 IMP
    hypoxia-inducible factor-1alpha signaling pathway GO:0097411 IMP
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrial matrix GO:0005759 TAS
    pyruvate dehydrogenase complex GO:0045254 IEA
 Experiment description of studies that identified PDK1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PDK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CRBN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 NME2P1 283458
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 DENR 8562
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
5 RASSF8 11228
Affinity Capture-MS Homo sapiens
6 SLC22A2 6582
Affinity Capture-MS Homo sapiens
7 LAMP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 HCST  
Affinity Capture-MS Homo sapiens
9 OXLD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 RPA2 6118
Proximity Label-MS Homo sapiens
11 TTR 7276
Affinity Capture-MS Homo sapiens
12 TRUB2  
Proximity Label-MS Homo sapiens
13 PAK2 5062
Affinity Capture-MS Homo sapiens
14 IGHM 3507
Affinity Capture-MS Homo sapiens
15 FPR1  
Affinity Capture-MS Homo sapiens
16 SPG7  
Affinity Capture-MS Homo sapiens
17 LRPPRC 10128
Proximity Label-MS Homo sapiens
18 SRCAP  
Affinity Capture-MS Homo sapiens
19 PMPCA 23203
Proximity Label-MS Homo sapiens
20 VKORC1 79001
Affinity Capture-MS Homo sapiens
21 HLA-B 3106
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 HINT2 84681
Proximity Label-MS Homo sapiens
23 MRPS26 64949
Proximity Label-MS Homo sapiens
24 MALSU1  
Affinity Capture-MS Homo sapiens
25 PDK2 5164
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 GRPR  
Affinity Capture-MS Homo sapiens
27 FOXD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 MECP2 4204
Affinity Capture-MS Homo sapiens
29 NSFL1C 55968
Affinity Capture-MS Homo sapiens
30 PKN2 5586
Affinity Capture-Western Homo sapiens
31 VWA8 23078
Affinity Capture-MS Homo sapiens
32 GPR17 2840
Affinity Capture-MS Homo sapiens
33 RALGDS  
Affinity Capture-Western Homo sapiens
34 FOXP1 27086
Co-localization Homo sapiens
35 SSR2  
Affinity Capture-MS Homo sapiens
36 MDH2 4191
Proximity Label-MS Homo sapiens
37 ATG4A  
Affinity Capture-MS Homo sapiens
38 C6orf203  
Proximity Label-MS Homo sapiens
39 FASTKD5  
Proximity Label-MS Homo sapiens
40 PARK2  
Affinity Capture-MS Homo sapiens
41 CXCR4 7852
Affinity Capture-MS Homo sapiens
42 HSCB 150274
Proximity Label-MS Homo sapiens
43 RPS6KB1 6198
Reconstituted Complex Homo sapiens
44 HMGN1  
Affinity Capture-MS Homo sapiens
45 PDHX 8050
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 GFM1 85476
Proximity Label-MS Homo sapiens
47 PYCRL 65263
Affinity Capture-MS Homo sapiens
48 PKN1 5585
Affinity Capture-Western Homo sapiens
49 MTRF1L  
Proximity Label-MS Homo sapiens
50 COG4 25839
Affinity Capture-MS Homo sapiens
51 NRBP2  
Affinity Capture-MS Homo sapiens
52 PCDHGB1  
Affinity Capture-MS Homo sapiens
53 RAMP2  
Affinity Capture-MS Homo sapiens
54 APLNR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 USP4 7375
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
56 MRRF  
Proximity Label-MS Homo sapiens
57 MTIF2 4528
Proximity Label-MS Homo sapiens
58 C9orf78 51759
Affinity Capture-MS Homo sapiens
59 ILKAP 80895
Affinity Capture-MS Homo sapiens
60 MTIF3  
Proximity Label-MS Homo sapiens
61 NPTN 27020
Affinity Capture-MS Homo sapiens
62 NPY2R  
Affinity Capture-MS Homo sapiens
63 SLCO4C1 353189
Affinity Capture-MS Homo sapiens
64 DHX30 22907
Proximity Label-MS Homo sapiens
65 PYCR2 29920
Affinity Capture-MS Homo sapiens
66 CELF2 10659
Affinity Capture-MS Homo sapiens
67 LAMTOR5 10542
Two-hybrid Homo sapiens
68 EPHA2 1969
Proximity Label-MS Homo sapiens
69 MYC  
Dosage Lethality Homo sapiens
70 RPA3 6119
Proximity Label-MS Homo sapiens
71 COL4A5  
Affinity Capture-MS Homo sapiens
72 EGFR 1956
Affinity Capture-MS Homo sapiens
73 MTOR 2475
Phenotypic Enhancement Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
74 PIK3CA 5290
PCA Homo sapiens
75 ACAD9 28976
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 SGK1  
Biochemical Activity Homo sapiens
77 AKT1 207
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
78 SLC22A4 6583
Affinity Capture-MS Homo sapiens
79 DLAT 1737
Reconstituted Complex Homo sapiens
80 METTL15  
Proximity Label-MS Homo sapiens
81 MCUR1 63933
Proximity Label-MS Homo sapiens
82 AVPR2  
Affinity Capture-MS Homo sapiens
83 CLPP 8192
Proximity Label-MS Homo sapiens
84 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
85 TNK2 10188
Affinity Capture-Western Homo sapiens
86 ZNFX1 57169
Affinity Capture-MS Homo sapiens
87 AUH 549
Proximity Label-MS Homo sapiens
88 SLC18A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 PDK3 5165
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 RIMS1  
Affinity Capture-MS Homo sapiens
91 MKNK1  
Affinity Capture-MS Homo sapiens
92 ICT1 3396
Proximity Label-MS Homo sapiens
93 LONP1 9361
Proximity Label-MS Homo sapiens
94 KAT2B  
Biochemical Activity Homo sapiens
95 PDHA1 5160
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
96 RPTOR 57521
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
97 TRMT61B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 NDUFS7 374291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 PDHB 5162
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 COX8A  
Proximity Label-MS Homo sapiens
101 MYH1  
Affinity Capture-MS Homo sapiens
102 C21orf33  
Proximity Label-MS Homo sapiens
103 NIPSNAP3B 55335
Affinity Capture-MS Homo sapiens
104 CCDC93 54520
Affinity Capture-MS Homo sapiens
105 SSBP1 6742
Proximity Label-MS Homo sapiens
106 CD80 941
Affinity Capture-MS Homo sapiens
107 TBRG4 9238
Proximity Label-MS Homo sapiens
108 AARS2  
Proximity Label-MS Homo sapiens
109 HLA-DPA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
110 STXBP2 6813
Affinity Capture-MS Homo sapiens
111 PDK4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 GRSF1 2926
Proximity Label-MS Homo sapiens
113 C1QBP 708
Proximity Label-MS Homo sapiens
114 XRCC6 2547
Two-hybrid Homo sapiens
115 METTL17  
Proximity Label-MS Homo sapiens
116 PVRL3 25945
Affinity Capture-MS Homo sapiens
117 TRIM47 91107
Affinity Capture-MS Homo sapiens
118 OTX2  
Affinity Capture-MS Homo sapiens
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