Gene description for TNK2
Gene name tyrosine kinase, non-receptor, 2
Gene symbol TNK2
Other names/aliases ACK
ACK-1
ACK1
p21cdc42Hs
Species Homo sapiens
 Database cross references - TNK2
ExoCarta ExoCarta_10188
Vesiclepedia VP_10188
Entrez Gene 10188
HGNC 19297
MIM 606994
UniProt Q07912  
 TNK2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
 Gene ontology annotations for TNK2
Molecular Function
    hormone receptor binding GO:0051427 IBA
    protein tyrosine kinase activity GO:0004713 IDA
    epidermal growth factor receptor binding GO:0005154 IDA
    WW domain binding GO:0050699 ISS
    GTPase inhibitor activity GO:0005095 TAS
    protein serine/threonine/tyrosine kinase activity GO:0004712 IDA
    ATP binding GO:0005524 IEA
    identical protein binding GO:0042802 IPI
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IEA
    protein serine/threonine kinase activity GO:0004674 IEA
    metal ion binding GO:0046872 IEA
    protein binding GO:0005515 IPI
Biological Process
    peptidyl-tyrosine autophosphorylation GO:0038083 IBA
    negative regulation of GTPase activity GO:0034260 TAS
    regulation of clathrin-mediated endocytosis GO:2000369 IDA
    cell differentiation GO:0030154 IBA
    cell surface receptor signaling pathway GO:0007166 TAS
    regulation of cell proliferation GO:0042127 IBA
    innate immune response GO:0045087 IBA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IDA
    small GTPase mediated signal transduction GO:0007264 TAS
    endocytosis GO:0006897 IEA
    transmembrane receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    phosphorylation GO:0016310 IDA
    cell migration GO:0016477 IBA
Subcellular Localization
    adherens junction GO:0005912 IEA
    extrinsic component of cytoplasmic side of plasma membrane GO:0031234 IBA
    nucleus GO:0005634 IDA
    clathrin-coated vesicle GO:0030136 IDA
    plasma membrane GO:0005886 IDA
    cytoplasmic vesicle membrane GO:0030659 IEA
    endosome GO:0005768 ISS
    axon GO:0030424 IEA
    Grb2-EGFR complex GO:0070436 IDA
    membrane GO:0016020 IDA
    dendrite GO:0030425 IEA
    coated pit GO:0005905 IDA
    neuronal cell body GO:0043025 IEA
    cytoplasm GO:0005737 IDA
    growth cone GO:0030426 IEA
 Experiment description of studies that identified TNK2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for TNK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDC42 998
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
2 CLTC 1213
Reconstituted Complex Homo sapiens
3 NCK1 4690
Affinity Capture-MS Homo sapiens
4 HSH2D 84941
Invivo Homo sapiens
Two-hybrid Homo sapiens
5 FYN 2534
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
6 GRB2 2885
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 MCF2  
Invitro Homo sapiens
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which TNK2 is involved
No pathways found





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