Gene description for TNK2
Gene name tyrosine kinase, non-receptor, 2
Gene symbol TNK2
Other names/aliases ACK
ACK-1
ACK1
p21cdc42Hs
Species Homo sapiens
 Database cross references - TNK2
ExoCarta ExoCarta_10188
Vesiclepedia VP_10188
Entrez Gene 10188
HGNC 19297
MIM 606994
UniProt Q07912  
 TNK2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
 Gene ontology annotations for TNK2
Molecular Function
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein serine/threonine/tyrosine kinase activity GO:0004712 IDA
    protein tyrosine kinase activity GO:0004713 IBA
    protein tyrosine kinase activity GO:0004713 IDA
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IEA
    GTPase inhibitor activity GO:0005095 TAS
    epidermal growth factor receptor binding GO:0005154 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ubiquitin protein ligase binding GO:0031625 IPI
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3Y41 kinase activity GO:0035401 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    identical protein binding GO:0042802 IPI
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    metal ion binding GO:0046872 IEA
    WW domain binding GO:0050699 ISS
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    endocytosis GO:0006897 IEA
    cell surface receptor signaling pathway GO:0007166 TAS
    small GTPase-mediated signal transduction GO:0007264 TAS
    phosphorylation GO:0016310 IDA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IDA
    regulation of clathrin-dependent endocytosis GO:2000369 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    endosome GO:0005768 ISS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    clathrin-coated pit GO:0005905 IDA
    adherens junction GO:0005912 IEA
    membrane GO:0016020 HDA
    clathrin-coated vesicle GO:0030136 IDA
    cytoplasmic vesicle membrane GO:0030659 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    Grb2-EGFR complex GO:0070436 IDA
    cytoophidium GO:0097268 IDA
 Experiment description of studies that identified TNK2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for TNK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SIAH1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 ATG16L1 55054
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
3 BAG3 9531
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
4 AXL 558
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 GTSE1 51512
Affinity Capture-MS Homo sapiens
6 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
7 TNK2 10188
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
8 ISG15 9636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 NCK2 8440
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 GALT 2592
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
11 NCK1 4690
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
12 KAT8  
Affinity Capture-Western Homo sapiens
13 NEDD4 4734
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
14 CDKN1B 1027
Affinity Capture-Western Homo sapiens
15 GRB2 2885
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
16 HECW1  
Reconstituted Complex Homo sapiens
17 SIAH2 6478
Affinity Capture-Western Homo sapiens
18 TP53 7157
Affinity Capture-Western Homo sapiens
19 EEA1 8411
Co-localization Homo sapiens
20 AKT1 207
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 FABP4 2167
Affinity Capture-Western Homo sapiens
22 Nedd4l 291553
Reconstituted Complex Rattus norvegicus
23 WWOX 51741
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
24 CLTA 1211
Affinity Capture-Western Homo sapiens
25 Amph  
Reconstituted Complex Rattus norvegicus
26 ATG9A 79065
Proximity Label-MS Homo sapiens
27 MCF2  
Affinity Capture-Western Homo sapiens
28 ALK 238
Affinity Capture-Western Homo sapiens
29 BCAR1 9564
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
30 GAK 2580
Affinity Capture-MS Homo sapiens
31 NEK6 10783
Two-hybrid Homo sapiens
32 MERTK 10461
Affinity Capture-Western Homo sapiens
33 SMURF1 57154
Reconstituted Complex Homo sapiens
34 SQSTM1 8878
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
35 CTTN 2017
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
36 EGFR 1956
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
37 NPHP1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
38 HSP90AB3P 3327
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 ZBTB16  
Affinity Capture-Western Homo sapiens
40 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
41 KRTAP10-3  
Two-hybrid Homo sapiens
42 NBR1 4077
Co-localization Homo sapiens
43 TP73  
Affinity Capture-Western Homo sapiens
44 Fabp4 79451
Reconstituted Complex Rattus norvegicus
45 LYN 4067
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
46 SNX9 51429
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
47 PDK1 5163
Affinity Capture-Western Homo sapiens
48 CDC42 998
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
49 AMPH 273
Affinity Capture-Western Homo sapiens
50 WWP2 11060
Reconstituted Complex Homo sapiens
51 WAS 7454
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
52 HCK 3055
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
53 Clta 83800
Reconstituted Complex Rattus norvegicus
54 Bin2  
Reconstituted Complex Rattus norvegicus
55 MAGI3 260425
Reconstituted Complex Homo sapiens
56 HSH2D 84941
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
57 FYN 2534
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
58 Uso1 56041
Affinity Capture-MS Mus musculus
59 LTK 4058
Affinity Capture-Western Homo sapiens
60 SH2D1A 4068
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
61 NTRK1 4914
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
62 FGFR1 2260
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
63 NTRK3 4916
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
64 BIN1 274
Affinity Capture-Western Homo sapiens
65 KAT5  
Affinity Capture-Western Homo sapiens
66 YES1 7525
Reconstituted Complex Homo sapiens
67 TRIM50  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
68 KCNA5  
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
69 NOTCH2NL 388677
Two-hybrid Homo sapiens
70 SRC 6714
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
71 AR 367
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
72 CLTC 1213
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
73 NTRK2 4915
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
74 KDM3A  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
75 AQP1 358
Two-hybrid Homo sapiens
76 CDC37 11140
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 Sec24c  
Affinity Capture-MS Mus musculus
78 NEDD4L 23327
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
79 PDGFRB 5159
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
80 EP300 2033
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which TNK2 is involved
PathwayEvidenceSource
Signal Transduction TAS Reactome
Signaling by LTK TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome





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