Gene description for Nedd4l
Gene name neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase
Gene symbol Nedd4l
Other names/aliases Nedd4-2
Species Rattus norvegicus
 Database cross references - Nedd4l
ExoCarta ExoCarta_291553
Entrez Gene 291553
 Nedd4l identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Nedd4l
Molecular Function
    ligase activity GO:0016874 IEA
    sodium channel regulator activity GO:0017080 ISO
    potassium channel regulator activity GO:0015459 ISO
    protein binding GO:0005515 IPI
    ion channel binding GO:0044325 ISO
    ubiquitin protein ligase activity GO:0061630 ISO
    potassium channel inhibitor activity GO:0019870 ISO
    sodium channel inhibitor activity GO:0019871 ISO
    ubiquitin-protein transferase activity GO:0004842 IDA
Biological Process
    protein monoubiquitination GO:0006513 IMP
    positive regulation of dendrite extension GO:1903861 ISO
    regulation of bicellular tight junction assembly GO:2000810 ISO
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 ISO
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 ISO
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 ISO
    negative regulation of potassium ion transmembrane transporter activity GO:1901017 ISO
    regulation of ion transmembrane transport GO:0034765 ISO
    negative regulation of sodium ion transmembrane transport GO:1902306 ISO
    regulation of membrane depolarization GO:0003254 ISO
    protein ubiquitination GO:0016567 ISO
    negative regulation of systemic arterial blood pressure GO:0003085 ISO
    positive regulation of caveolin-mediated endocytosis GO:2001288 IMP
    positive regulation of protein catabolic process GO:0045732 IMP
    protein K48-linked ubiquitination GO:0070936 ISO
    negative regulation of potassium ion transmembrane transport GO:1901380 ISO
    response to metal ion GO:0010038 ISO
    regulation of membrane potential GO:0042391 ISO
    positive regulation of sodium ion transport GO:0010765 ISO
    regulation of potassium ion transmembrane transporter activity GO:1901016 ISO
    positive regulation of cation channel activity GO:2001259 ISO
    negative regulation of sodium ion transmembrane transporter activity GO:2000650 ISO
    ventricular cardiac muscle cell action potential GO:0086005 IMP
    negative regulation of sodium ion transport GO:0010766 ISO
    negative regulation of protein localization to cell surface GO:2000009 ISO
    regulation of membrane repolarization GO:0060306 ISO
    response to salt stress GO:0009651 ISO
Subcellular Localization
    cellular_component GO:0005575 ND
    extracellular exosome GO:0070062 ISO
    plasma membrane GO:0005886 ISO
    cytoplasm GO:0005737 IBA
 Experiment description of studies that identified Nedd4l in exosomes
1
Experiment ID 225
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Nedd4l
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Nedd4l is involved
No pathways found





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