Experiment description of studies that identified AXL in exosomes |
1 |
Experiment ID |
226 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
GAPDH
|
EV Cytosolic markers |
✔
CD9|FLOT1
|
EV Membrane markers |
✔
LMNA|H2AFX|ATP5A1|TOMM20
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
mRNA
|
Identification method |
Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
|
PubMed ID |
26027894
|
Organism |
Homo sapiens |
Experiment description |
Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting |
Authors |
Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar |
Journal name |
Journal of Extracellular Vesicles
|
Publication year |
2015 |
Sample |
Endothelial cells |
Sample name |
HMEC-1 |
Isolation/purification methods |
Differential ultracentrifugation Sucrose density gradient |
Flotation density |
1.10 g/mL
|
Molecules identified in the study |
miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA |
Methods used in the study |
Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting |
|
|
2 |
Experiment ID |
260 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101
|
EV Cytosolic markers |
✔
FLOT1|CD81
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25950383
|
Organism |
Homo sapiens |
Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
Journal name |
Pigment Cell Melanoma Res
|
Publication year |
2015 |
Sample |
Melanoma cells |
Sample name |
1205Lu |
Isolation/purification methods |
Differential centrifugation Unltracentrifugation Sucrose density gradient |
Flotation density |
1.13 - 1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
3 |
Experiment ID |
126 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
GAPDH
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [LTQ-FT Ultra]
|
PubMed ID |
Unpublished / Not applicable
|
Organism |
Homo sapiens |
Experiment description |
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? |
Authors |
Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim |
Journal name |
Mesenchymal Stem Cell Therapy
|
Publication year |
2011 |
Sample |
Mesenchymal stem cells |
Sample name |
huES9.E1 |
Isolation/purification methods |
HPLC |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Antobody array Mass spectrometry |
|
|