Gene description for NEFL
Gene name neurofilament, light polypeptide
Gene symbol NEFL
Other names/aliases CMT1F
CMT2E
NF-L
NF68
NFL
PPP1R110
Species Homo sapiens
 Database cross references - NEFL
ExoCarta ExoCarta_4747
Entrez Gene 4747
HGNC 7739
MIM 162280
UniProt P07196  
 NEFL identified in exosomes derived from the following tissue/cell type
Liver cancer cells 25265333    
 Gene ontology annotations for NEFL
Molecular Function
    protein binding GO:0005515 IPI
    protein binding, bridging GO:0030674 ISS
    phospholipase binding GO:0043274 IEA
    protein C-terminus binding GO:0008022 IPI
    protein domain specific binding GO:0019904 IEA
    structural constituent of cytoskeleton GO:0005200 IDA
    identical protein binding GO:0042802 IPI
Biological Process
    axon transport of mitochondrion GO:0019896 IMP
    intermediate filament organization GO:0045109 IMP
    anterograde axon cargo transport GO:0008089 IMP
    neuron projection morphogenesis GO:0048812 IEA
    regulation of axon diameter GO:0031133 IEA
    positive regulation of axonogenesis GO:0050772 IEA
    neuromuscular process controlling balance GO:0050885 IEA
    locomotion GO:0040011 IEA
    synaptic transmission GO:0007268 TAS
    retrograde axon cargo transport GO:0008090 IMP
    microtubule cytoskeleton organization GO:0000226 IEA
    response to corticosterone GO:0051412 IEA
    neurofilament bundle assembly GO:0033693 IMP
    peripheral nervous system axon regeneration GO:0014012 IEA
    response to toxic substance GO:0009636 IEA
    negative regulation of neuron apoptotic process GO:0043524 IEA
    response to peptide hormone GO:0043434 IEA
    protein polymerization GO:0051258 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 ISS
    TSC1-TSC2 complex GO:0033596 IDA
    growth cone GO:0030426 IEA
    neurofilament GO:0005883 IDA
    axon GO:0030424 IDA
 Experiment description of studies that identified NEFL in exosomes
1
Experiment ID 267
ISEV standards
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EV Membrane markers
CANX|HSP90B1
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh‑7 Cells Using SILAC Labeling and LC−MS/MS
Authors Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for NEFL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PKN1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
2 NEFH 4744
Co-purification Homo sapiens
3 SPTAN1 6709
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
4 TSC1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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