Gene description for FGD4
Gene name FYVE, RhoGEF and PH domain containing 4
Gene symbol FGD4
Other names/aliases CMT4H
FRABP
ZFYVE6
Species Homo sapiens
 Database cross references - FGD4
ExoCarta ExoCarta_121512
Vesiclepedia VP_121512
Entrez Gene 121512
HGNC 19125
MIM 611104
UniProt Q96M96  
 FGD4 identified in sEVs derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for FGD4
Molecular Function
    actin binding GO:0003779 IEA
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 ISS
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    small GTPase binding GO:0031267 ISS
    metal ion binding GO:0046872 IEA
Biological Process
    cytoskeleton organization GO:0007010 IBA
    cytoskeleton organization GO:0007010 ISS
    regulation of cell shape GO:0008360 ISS
    actin cytoskeleton organization GO:0030036 ISS
    regulation of GTPase activity GO:0043087 ISS
    filopodium assembly GO:0046847 IBA
    filopodium assembly GO:0046847 ISS
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
Subcellular Localization
    ruffle GO:0001726 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISS
    Golgi apparatus GO:0005794 ISS
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
    lamellipodium GO:0030027 ISS
    filopodium GO:0030175 IEA
 Experiment description of studies that identified FGD4 in sEVs
1
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for FGD4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GSN 2934
Affinity Capture-MS Homo sapiens
2 PLEC 5339
Affinity Capture-MS Homo sapiens
3 C9orf16  
Two-hybrid Homo sapiens
4 CDC42 998
FRET Homo sapiens
5 ACTB 60
Proximity Label-MS Homo sapiens
6 NEFL 4747
Affinity Capture-MS Homo sapiens
7 CCDC86  
Affinity Capture-MS Homo sapiens
8 FGD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 CUL3 8452
Affinity Capture-MS Homo sapiens
10 CAPZA2 830
Affinity Capture-MS Homo sapiens
11 DNAJC9 23234
Affinity Capture-MS Homo sapiens
12 PDIA3 2923
Affinity Capture-MS Homo sapiens
13 Myh9 17886
Affinity Capture-MS Mus musculus
14 POTEE 445582
Affinity Capture-MS Homo sapiens
15 PARK2  
Affinity Capture-MS Homo sapiens
16 KIF14 9928
Affinity Capture-MS Homo sapiens
17 PRKCA 5578
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 CTDSPL2  
Affinity Capture-MS Homo sapiens
19 LANCL1 10314
Affinity Capture-MS Homo sapiens
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