Gene description for PPP1R18
Gene name protein phosphatase 1, regulatory subunit 18
Gene symbol PPP1R18
Other names/aliases HKMT1098
KIAA1949
Species Homo sapiens
 Database cross references - PPP1R18
ExoCarta ExoCarta_170954
Vesiclepedia VP_170954
Entrez Gene 170954
HGNC 29413
MIM 610990
UniProt Q6NYC8  
 PPP1R18 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PPP1R18
Molecular Function
    actin binding GO:0003779 IEA
    protein binding GO:0005515 IPI
    phosphatase binding GO:0019902 IEA
Subcellular Localization
    cytoplasm GO:0005737 IEA
    cytoskeleton GO:0005856 IEA
 Experiment description of studies that identified PPP1R18 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PPP1R18
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LIG4 3981
Affinity Capture-MS Homo sapiens
2 HOMER3 9454
Two-hybrid Homo sapiens
3 LUZP4  
Affinity Capture-MS Homo sapiens
4 CCDC88B 283234
Two-hybrid Homo sapiens
5 PPP1CB 5500
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 PPP1R3A  
Affinity Capture-MS Homo sapiens
7 Tpm1 22003
Affinity Capture-MS Mus musculus
8 ABRA  
Two-hybrid Homo sapiens
9 CARD9 64170
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 SPERT  
Two-hybrid Homo sapiens
11 Calml3  
Affinity Capture-MS Mus musculus
12 FAM9B  
Two-hybrid Homo sapiens
13 TEX11  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
14 GFAP 2670
Two-hybrid Homo sapiens
15 BRCA1 672
Affinity Capture-MS Homo sapiens
16 PLEKHF2 79666
Two-hybrid Homo sapiens
17 KIF20A 10112
Affinity Capture-MS Homo sapiens
18 TRIM62  
Two-hybrid Homo sapiens
19 Actb 11461
Affinity Capture-MS Mus musculus
20 CCDC102B  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
21 STX11 8676
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 PCBP3 54039
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 TRIM54  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
24 PPP1CC 5501
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 SEPT9 10801
Affinity Capture-MS Homo sapiens
26 CEP70  
Two-hybrid Homo sapiens
27 DYRK1A 1859
Affinity Capture-MS Homo sapiens
28 KRT40  
Two-hybrid Homo sapiens
29 IQGAP1 8826
Affinity Capture-MS Homo sapiens
30 CALCOCO2  
Two-hybrid Homo sapiens
31 TFIP11  
Two-hybrid Homo sapiens
32 Taok2  
Affinity Capture-MS Mus musculus
33 KRT31 3881
Two-hybrid Homo sapiens
34 MID2 11043
Two-hybrid Homo sapiens
35 ATG16L1 55054
Affinity Capture-MS Homo sapiens
36 FSD2 123722
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
37 Lima1  
Affinity Capture-MS Mus musculus
38 KIF14 9928
Affinity Capture-MS Homo sapiens
39 GOLGA6L9  
Two-hybrid Homo sapiens
40 PPP1CA 5499
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 MTUS2 23281
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
42 KRT34  
Two-hybrid Homo sapiens
43 MYO18A 399687
Affinity Capture-MS Homo sapiens
44 FHL5 9457
Two-hybrid Homo sapiens
45 PRPH 5630
Two-hybrid Homo sapiens
46 CTDP1 9150
Affinity Capture-MS Homo sapiens
47 SPDL1  
Two-hybrid Homo sapiens
48 CDR2L  
Two-hybrid Homo sapiens
49 GOLGA2 2801
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
50 CPVL 54504
Affinity Capture-MS Homo sapiens
51 CDH1 999
Proximity Label-MS Homo sapiens
52 CHMP4B 128866
Affinity Capture-MS Homo sapiens
53 GCC1 79571
Two-hybrid Homo sapiens
54 VCP 7415
Affinity Capture-MS Homo sapiens
55 Pafah1b1 18472
Affinity Capture-MS Mus musculus
56 MEOX2  
Two-hybrid Homo sapiens
57 BICD2 23299
Two-hybrid Homo sapiens
58 ACTB 60
Proximity Label-MS Homo sapiens
59 DBN1 1627
Affinity Capture-MS Homo sapiens
60 HAP1  
Two-hybrid Homo sapiens
61 GTPBP3  
Two-hybrid Homo sapiens
62 ANLN 54443
Affinity Capture-MS Homo sapiens
63 LIMA1 51474
Affinity Capture-MS Homo sapiens
64 HNRNPD 3184
Affinity Capture-MS Homo sapiens
65 Myh9 17886
Affinity Capture-MS Mus musculus
66 KRT33B 3884
Two-hybrid Homo sapiens
67 KCTD9  
Two-hybrid Homo sapiens
68 ECT2 1894
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 DOCK7 85440
Affinity Capture-MS Homo sapiens
70 IKZF3  
Two-hybrid Homo sapiens
71 CCDC57 284001
Two-hybrid Homo sapiens
72 DES 1674
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
73 RASIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 MYO19  
Affinity Capture-MS Homo sapiens
75 CCDC155  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
76 ACTN3 89
Two-hybrid Homo sapiens
77 KRT27 342574
Two-hybrid Homo sapiens
78 LZTS2 84445
Two-hybrid Homo sapiens
79 KRT15 3866
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
80 COG6 57511
Two-hybrid Homo sapiens
81 SYNPO 11346
Affinity Capture-MS Homo sapiens
82 NEFL 4747
Two-hybrid Homo sapiens
83 MYC  
Affinity Capture-MS Homo sapiens
84 TRIM27  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
85 SORBS3 10174
Two-hybrid Homo sapiens
86 DZIP1  
Two-hybrid Homo sapiens
87 MRPL38  
Two-hybrid Homo sapiens
88 KRT19 3880
Two-hybrid Homo sapiens
89 TSGA10  
Two-hybrid Homo sapiens
90 MGEA5 10724
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 GSN 2934
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 TRAF2 7186
Two-hybrid Homo sapiens
93 KRT16 3868
Two-hybrid Homo sapiens
94 KRT36 8689
Two-hybrid Homo sapiens
95 VPS52 6293
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
96 EZR 7430
Proximity Label-MS Homo sapiens
97 CAPZA2 830
Affinity Capture-MS Homo sapiens
98 TSC1 7248
Two-hybrid Homo sapiens
99 DDX58 23586
Affinity Capture-RNA Homo sapiens
100 TCF4  
Two-hybrid Homo sapiens
101 TRIM23 373
Two-hybrid Homo sapiens
102 FLNA 2316
Affinity Capture-MS Homo sapiens
103 CIT 11113
Affinity Capture-MS Homo sapiens
104 TRIM69 140691
Two-hybrid Homo sapiens
105 LDOC1  
Two-hybrid Homo sapiens
106 PDE4DIP 9659
Two-hybrid Homo sapiens
107 KRT13 3860
Two-hybrid Homo sapiens
108 Flnb 286940
Affinity Capture-MS Mus musculus
109 CCDC136  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
110 HSF2BP  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which PPP1R18 is involved
No pathways found





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