Gene description for ARHGEF25
Gene name Rho guanine nucleotide exchange factor (GEF) 25
Gene symbol ARHGEF25
Other names/aliases GEFT
p63RhoGEF
Species Homo sapiens
 Database cross references - ARHGEF25
ExoCarta ExoCarta_115557
Vesiclepedia VP_115557
Entrez Gene 115557
HGNC 30275
MIM 610215
UniProt Q86VW2  
 ARHGEF25 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ARHGEF25
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
Biological Process
    axon guidance GO:0007411 IBA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISS
    extrinsic component of membrane GO:0019898 IBA
    myofibril GO:0030016 ISS
    sarcomere GO:0030017 IEA
 Experiment description of studies that identified ARHGEF25 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ARHGEF25
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HBB 3043
Affinity Capture-MS Homo sapiens
2 CBX1 10951
Affinity Capture-MS Homo sapiens
3 RHOU 58480
Reconstituted Complex Homo sapiens
4 PLEC 5339
Affinity Capture-MS Homo sapiens
5 CCDC86  
Affinity Capture-MS Homo sapiens
6 ARPC1B 10095
Affinity Capture-MS Homo sapiens
7 RTN4R 65078
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SMARCE1 6605
Affinity Capture-MS Homo sapiens
9 PPP1R9B 84687
Affinity Capture-MS Homo sapiens
10 FUBP3 8939
Affinity Capture-MS Homo sapiens
11 CDC42 998
Reconstituted Complex Homo sapiens
12 PRKCG 5582
Reconstituted Complex Homo sapiens
13 POTEE 445582
Affinity Capture-MS Homo sapiens
14 CORO2B 10391
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 PPP1R12A 4659
Affinity Capture-MS Homo sapiens
16 NEFL 4747
Affinity Capture-MS Homo sapiens
17 DNAJC9 23234
Affinity Capture-MS Homo sapiens
18 C14orf166 51637
Affinity Capture-MS Homo sapiens
19 GABRB2 2561
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 LMO1  
Affinity Capture-MS Homo sapiens
21 RAC1 5879
Reconstituted Complex Homo sapiens
22 RHOA 387
Reconstituted Complex Homo sapiens
FRET Homo sapiens
Biochemical Activity Homo sapiens
23 FAM207A  
Affinity Capture-MS Homo sapiens
24 LMO3  
Affinity Capture-MS Homo sapiens
25 FLOT1 10211
Affinity Capture-MS Homo sapiens
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