Gene description for DCAF12
Gene name DDB1 and CUL4 associated factor 12
Gene symbol DCAF12
Other names/aliases CT102
KIAA1892
TCC52
WDR40A
Species Homo sapiens
 Database cross references - DCAF12
ExoCarta ExoCarta_25853
Vesiclepedia VP_25853
Entrez Gene 25853
HGNC 19911
UniProt Q5T6F0  
 DCAF12 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for DCAF12
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin-like ligase-substrate adaptor activity GO:1990756 IDA
Biological Process
    regulation of autophagy GO:0010506 IDA
    protein ubiquitination GO:0016567 IEA
    T cell activation GO:0042110 IDA
    ubiquitin-dependent protein catabolic process via the C-end degron rule pathway GO:0140627 IDA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IDA
    Cul4-RING E3 ubiquitin ligase complex GO:0080008 IBA
    Cul4-RING E3 ubiquitin ligase complex GO:0080008 IDA
    Cul4-RING E3 ubiquitin ligase complex GO:0080008 IMP
 Experiment description of studies that identified DCAF12 in exosomes
1
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for DCAF12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TUBA3C 7278
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 VPS35 55737
Affinity Capture-MS Homo sapiens
3 BIRC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 CUL1 8454
Affinity Capture-MS Homo sapiens
5 XIAP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 CCT3 7203
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 PDCL3 79031
Affinity Capture-MS Homo sapiens
8 MAGEA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
9 TP53 7157
Affinity Capture-MS Homo sapiens
10 CCT2 10576
Affinity Capture-MS Homo sapiens
11 GOLIM4 27333
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 Cdk7  
Affinity Capture-Western Drosophila melanogaster
13 ZZEF1 23140
Affinity Capture-MS Homo sapiens
14 YY1AP1 55249
Affinity Capture-MS Homo sapiens
15 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
16 CUL4B 8450
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
17 Ddb1 13194
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
18 DDX58 23586
Affinity Capture-RNA Homo sapiens
19 CCT7 10574
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 CCT5 22948
Affinity Capture-MS Homo sapiens
21 BIRC6 57448
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 PFDN2 5202
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 DDA1  
Affinity Capture-MS Homo sapiens
24 MOV10 4343
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 YAP1 10413
Affinity Capture-Western Homo sapiens
26 MCMBP 79892
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 WWTR1 25937
Affinity Capture-Western Homo sapiens
28 COPS6 10980
Affinity Capture-MS Homo sapiens
29 COPS5 10987
Affinity Capture-MS Homo sapiens
30 SHARPIN  
Affinity Capture-MS Homo sapiens
31 DDB1 1642
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
32 CUL4A 8451
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DCAF12 is involved
No pathways found





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