Gene description for Ruvbl2
Gene name RuvB-like protein 2
Gene symbol Ruvbl2
Other names/aliases mp47
p47
Species Mus musculus
 Database cross references - Ruvbl2
ExoCarta ExoCarta_20174
Vesiclepedia VP_20174
Entrez Gene 20174
UniProt Q9WTM5  
 Ruvbl2 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ruvbl2
Molecular Function
    helicase activity GO:0004386 IEA
    ATPase activity GO:0016887 ISO
    identical protein binding GO:0042802 ISO
    hydrolase activity GO:0016787 IEA
    ATP-dependent 5'-3' DNA helicase activity GO:0043141 IEA
    nucleotide binding GO:0000166 IEA
    DNA helicase activity GO:0003678 ISO
    damaged DNA binding GO:0003684 IEA
    chromatin DNA binding GO:0031490 ISO
    RNA polymerase II distal enhancer sequence-specific DNA binding GO:0000980 ISO
    RNA polymerase II core promoter sequence-specific DNA binding GO:0000979 ISO
    ATP binding GO:0005524 IEA
Biological Process
    DNA repair GO:0006281 IEA
    negative regulation of estrogen receptor binding GO:0071899 ISO
    histone H2A acetylation GO:0043968 ISO
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    establishment of protein localization to chromatin GO:0071169 ISO
    cellular response to UV GO:0034644 ISO
    positive regulation of histone acetylation GO:0035066 ISO
    regulation of transcription, DNA-templated GO:0006355 IEA
    DNA recombination GO:0006310 IEA
    DNA duplex unwinding GO:0032508 ISO
    cellular response to estradiol stimulus GO:0071392 ISO
    transcriptional activation by promoter-enhancer looping GO:0071733 ISO
    chromatin modification GO:0016568 IEA
    transcription, DNA-templated GO:0006351 IEA
    chromatin remodeling GO:0006338 ISO
    histone H4 acetylation GO:0043967 ISO
    cellular response to DNA damage stimulus GO:0006974 IEA
Subcellular Localization
    nucleus GO:0005634 ISO
    NuA4 histone acetyltransferase complex GO:0035267 ISO
    nuclear euchromatin GO:0005719 ISO
    MLL1 complex GO:0071339 ISO
    Swr1 complex GO:0000812 ISO
    intracellular GO:0005622 ISO
    cytoplasm GO:0005737 ISO
    nucleoplasm GO:0005654 ISO
    extracellular exosome GO:0070062 ISO
    Ino80 complex GO:0031011 ISO
    ribonucleoprotein complex GO:0030529 IDA
 Experiment description of studies that identified Ruvbl2 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD81|FLOT1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ruvbl2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ruvbl2 is involved
PathwayEvidenceSource
HATs acetylate histones IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here