Gene description for Wdr1
Gene name WD repeat domain 1
Gene symbol Wdr1
Other names/aliases Aip1
D5Wsu185e
rede
Species Mus musculus
 Database cross references - Wdr1
ExoCarta ExoCarta_22388
Vesiclepedia VP_22388
Entrez Gene 22388
UniProt O88342  
 Wdr1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Wdr1
Molecular Function
    actin binding GO:0003779 ISO
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 ISO
    actin filament binding GO:0051015 ISS
Biological Process
    neutrophil mediated immunity GO:0002446 IMP
    sensory perception of sound GO:0007605 ISO
    regulation of cell shape GO:0008360 IMP
    actin cytoskeleton organization GO:0030036 IMP
    actin filament depolymerization GO:0030042 IBA
    actin filament fragmentation GO:0030043 IMP
    platelet formation GO:0030220 IMP
    regulation of actin filament depolymerization GO:0030834 ISO
    positive regulation of actin filament depolymerization GO:0030836 IGI
    cortical cytoskeleton organization GO:0030865 IMP
    locomotion GO:0040011 IBA
    establishment of planar polarity of follicular epithelium GO:0042247 IMP
    apical junction assembly GO:0043297 IEA
    apical junction assembly GO:0043297 ISO
    maintenance of epithelial cell apical/basal polarity GO:0045199 IEA
    maintenance of epithelial cell apical/basal polarity GO:0045199 ISO
    sarcomere organization GO:0045214 IBA
    sarcomere organization GO:0045214 IMP
    regulation of oligodendrocyte differentiation GO:0048713 ISO
    regulation of ventricular cardiac muscle cell membrane repolarization GO:0060307 IMP
    neutrophil migration GO:1990266 IMP
Subcellular Localization
    podosome GO:0002102 IEA
    cytoplasm GO:0005737 IEA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    cell-cell junction GO:0005911 IEA
    cell-cell junction GO:0005911 ISO
    actin cytoskeleton GO:0015629 ISO
    cell junction GO:0030054 ISO
    cortical actin cytoskeleton GO:0030864 IBA
    cell projection GO:0042995 IEA
    myelin sheath GO:0043209 HDA
    synapse GO:0045202 IDA
    glutamatergic synapse GO:0098978 IDA
 Experiment description of studies that identified Wdr1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Wdr1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Ruvbl1 56505
Affinity Capture-MS Mus musculus
3 Leo1  
Affinity Capture-MS Mus musculus
4 Sf3b4 107701
Affinity Capture-MS Mus musculus
5 Ruvbl2 20174
Affinity Capture-MS Mus musculus
6 Eed  
Affinity Capture-MS Mus musculus
7 Mycbp2 105689
Affinity Capture-MS Mus musculus
8 Taldo1 21351
Co-fractionation Mus musculus
9 Mapt  
Affinity Capture-MS Mus musculus
10 Rbbp7 245688
Co-fractionation Mus musculus
11 Cdc73  
Affinity Capture-MS Mus musculus
12 Wdr61  
Affinity Capture-MS Mus musculus
13 Ddb1 13194
Affinity Capture-MS Mus musculus
14 Thop1  
Co-fractionation Mus musculus
15 VASP 7408
Affinity Capture-MS Homo sapiens
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