Gene description for Sf3b4
Gene name splicing factor 3b, subunit 4
Gene symbol Sf3b4
Other names/aliases SF3b49
SF3b50
Sap49
Species Mus musculus
 Database cross references - Sf3b4
ExoCarta ExoCarta_107701
Entrez Gene 107701
UniProt Q8QZY9  
 Sf3b4 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Sf3b4
Molecular Function
    poly(A) RNA binding GO:0044822 ISO
    nucleic acid binding GO:0003676 IEA
    nucleotide binding GO:0000166 IEA
    RNA binding GO:0003723 IEA
Biological Process
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IEA
    positive regulation of mRNA splicing, via spliceosome GO:0048026 IMP
Subcellular Localization
    U12-type spliceosomal complex GO:0005689 ISO
    spliceosomal complex GO:0005681 ISO
    nucleus GO:0005634 IEA
 Experiment description of studies that identified Sf3b4 in exosomes
1
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Sf3b4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Sf3b4 is involved
PathwayEvidenceSource
mRNA Splicing - Major Pathway IEA Reactome
mRNA Splicing - Minor Pathway IEA Reactome





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