Gene description for Sf3b4
Gene name splicing factor 3b, subunit 4
Gene symbol Sf3b4
Other names/aliases SF3b49
SF3b50
Sap49
Species Mus musculus
 Database cross references - Sf3b4
ExoCarta ExoCarta_107701
Vesiclepedia VP_107701
Entrez Gene 107701
UniProt Q8QZY9  
 Sf3b4 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Sf3b4
Molecular Function
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 IEA
    splicing factor binding GO:1990935 ISO
    splicing factor binding GO:1990935 ISS
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 IMP
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISS
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    spliceosomal complex GO:0005681 ISO
    U2-type spliceosomal complex GO:0005684 ISO
    U2-type spliceosomal complex GO:0005684 ISS
    U2 snRNP GO:0005686 IBA
    U12-type spliceosomal complex GO:0005689 IEA
    U12-type spliceosomal complex GO:0005689 ISO
    U2-type precatalytic spliceosome GO:0071005 IEA
    U2-type precatalytic spliceosome GO:0071005 ISO
    precatalytic spliceosome GO:0071011 IBA
 Experiment description of studies that identified Sf3b4 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Sf3b4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Zbtb7b  
Affinity Capture-MS Mus musculus
2 Wdr1 22388
Affinity Capture-MS Mus musculus
3 Prmt5 27374
Biochemical Activity Mus musculus
View the network image/svg+xml



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