Gene description for Prmt5
Gene name protein arginine N-methyltransferase 5
Gene symbol Prmt5
Other names/aliases Jbp1
Skb1
Species Mus musculus
 Database cross references - Prmt5
ExoCarta ExoCarta_27374
Vesiclepedia VP_27374
Entrez Gene 27374
UniProt Q8CIG8  
 Prmt5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Prmt5
Molecular Function
    p53 binding GO:0002039 ISO
    chromatin binding GO:0003682 IMP
    transcription corepressor activity GO:0003714 IMP
    protein binding GO:0005515 IPI
    methyltransferase activity GO:0008168 ISO
    methyl-CpG binding GO:0008327 ISO
    methyl-CpG binding GO:0008327 ISS
    histone arginine N-methyltransferase activity GO:0008469 IBA
    histone arginine N-methyltransferase activity GO:0008469 IEA
    protein-arginine N-methyltransferase activity GO:0016274 ISO
    protein-arginine N-methyltransferase activity GO:0016274 ISS
    protein-arginine omega-N symmetric methyltransferase activity GO:0035243 ISO
    protein-arginine omega-N symmetric methyltransferase activity GO:0035243 ISS
    histone H3R17 methyltransferase activity GO:0035642 IEA
    identical protein binding GO:0042802 ISO
    ribonucleoprotein complex binding GO:0043021 ISO
    histone H4R3 methyltransferase activity GO:0044020 IDA
    histone H4R3 methyltransferase activity GO:0044020 IMP
    histone H4R3 methyltransferase activity GO:0044020 ISO
    histone H4R3 methyltransferase activity GO:0044020 ISS
    protein-containing complex binding GO:0044877 ISO
    protein heterodimerization activity GO:0046982 ISO
    histone H3K37 methyltransferase activity GO:0062122 IEA
    histone H3R2 methyltransferase activity GO:0070611 IEA
    histone H2AR3 methyltransferase activity GO:0070612 IDA
    E-box binding GO:0070888 IDA
    histone H3R8 methyltransferase activity GO:0140592 IEA
    histone H3K56 methyltransferase activity GO:0140759 IEA
    histone H3R26 methyltransferase activity GO:0140903 IEA
    histone H4K12 methyltransferase activity GO:0140984 IEA
    histone H2AQ104 methyltransferase activity GO:1990259 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    spliceosomal snRNP assembly GO:0000387 ISO
    spliceosomal snRNP assembly GO:0000387 ISS
    spliceosomal snRNP assembly GO:0000387 NAS
    chromatin remodeling GO:0006338 IEA
    chromatin remodeling GO:0006338 ISO
    DNA-templated transcription termination GO:0006353 ISO
    DNA-templated transcription termination GO:0006353 ISS
    regulation of DNA-templated transcription GO:0006355 IBA
    gene expression GO:0010467 IDA
    regulation of gene expression GO:0010468 ISO
    peptidyl-arginine methylation GO:0018216 ISO
    peptidyl-arginine methylation GO:0018216 ISS
    circadian regulation of gene expression GO:0032922 IMP
    peptidyl-arginine N-methylation GO:0035246 ISO
    endothelial cell activation GO:0042118 ISO
    negative regulation of gene expression via chromosomal CpG island methylation GO:0044027 ISO
    negative regulation of cell differentiation GO:0045596 ISO
    positive regulation of mRNA splicing, via spliceosome GO:0048026 NAS
    positive regulation of oligodendrocyte differentiation GO:0048714 ISO
    regulation of ERK1 and ERK2 cascade GO:0070372 ISO
    Golgi ribbon formation GO:0090161 ISO
    Golgi ribbon formation GO:0090161 ISS
    positive regulation of adenylate cyclase-inhibiting dopamine receptor signaling pathway GO:1904992 ISO
Subcellular Localization
    chromatin GO:0000785 ISO
    male germ cell nucleus GO:0001673 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    protein-containing complex GO:0032991 ISO
    methylosome GO:0034709 ISO
    methylosome GO:0034709 ISS
    histone methyltransferase complex GO:0035097 IDA
    histone methyltransferase complex GO:0035097 ISO
 Experiment description of studies that identified Prmt5 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prmt5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sf3a3 75062
Co-fractionation Mus musculus
2 WIZ 58525
Affinity Capture-MS Homo sapiens
3 RIOK1 83732
Affinity Capture-MS Homo sapiens
4 WDR77 79084
Affinity Capture-MS Homo sapiens
5 NR0B2  
Affinity Capture-MS Homo sapiens
6 SNRPB 6628
Affinity Capture-MS Homo sapiens
7 Wdr77  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
8 Atxn1  
Affinity Capture-MS Mus musculus
9 Kctd13  
Affinity Capture-MS Mus musculus
10 Snrpd2 107686
Co-fractionation Mus musculus
11 Smarca4  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
12 Chtop  
Biochemical Activity Mus musculus
13 Gli1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
14 CLNS1A 1207
Affinity Capture-MS Homo sapiens
15 Serbp1 66870
Biochemical Activity Mus musculus
16 Myd88  
Affinity Capture-MS Mus musculus
17 Myod1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
18 Sf3b4 107701
Biochemical Activity Mus musculus
19 Hnrnpd 11991
Biochemical Activity Mus musculus
20 Pabpc1 18458
Biochemical Activity Mus musculus
21 Snrpb 20638
Biochemical Activity Mus musculus
22 Nanog  
Affinity Capture-MS Mus musculus
23 Snrpc 20630
Biochemical Activity Mus musculus
24 Nr0b2  
Affinity Capture-Western Mus musculus
Biochemical Activity Mus musculus
25 COPRS  
Affinity Capture-MS Homo sapiens
26 Atg16l1  
Affinity Capture-MS Mus musculus
27 Tcerg1  
Biochemical Activity Mus musculus
28 FARP1 10160
Affinity Capture-MS Homo sapiens
29 Fancd2  
Affinity Capture-MS Mus musculus
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