Gene description for Pabpc1
Gene name poly(A) binding protein, cytoplasmic 1
Gene symbol Pabpc1
Other names/aliases PABP
Pabp1
PabpI
Pabpl1
ePAB
Species Mus musculus
 Database cross references - Pabpc1
ExoCarta ExoCarta_18458
Entrez Gene 18458
UniProt P29341  
 Pabpc1 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Neural stem cells 25242146    
 Gene ontology annotations for Pabpc1
Molecular Function
    mRNA binding GO:0003729 ISO
    RNA binding GO:0003723 IEA
    protein C-terminus binding GO:0008022 ISO
    poly(A) binding GO:0008143 ISO
    poly(A) RNA binding GO:0044822 ISO
    nucleic acid binding GO:0003676 IEA
    protein binding GO:0005515 IPI
    nucleotide binding GO:0000166 IEA
    poly(U) RNA binding GO:0008266 ISO
Biological Process
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IEA
    positive regulation of nuclear-transcribed mRNA poly(A) tail shortening GO:0060213 IMP
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 IEA
    negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:2000623 ISO
    positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:1900153 IMP
    gene silencing by RNA GO:0031047 IMP
Subcellular Localization
    cytoplasmic stress granule GO:0010494 ISO
    extracellular exosome GO:0070062 ISO
    catalytic step 2 spliceosome GO:0071013 ISO
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    focal adhesion GO:0005925 ISO
    nucleus GO:0005634 ISO
    spliceosomal complex GO:0005681 IEA
    ribonucleoprotein complex GO:0030529 ISO
    membrane GO:0016020 ISO
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified Pabpc1 in exosomes
1
Experiment ID 214
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 264
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pabpc1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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