Gene description for MKRN1
Gene name makorin ring finger protein 1
Gene symbol MKRN1
Other names/aliases RNF61
Species Homo sapiens
 Database cross references - MKRN1
ExoCarta ExoCarta_23608
Vesiclepedia VP_23608
Entrez Gene 23608
HGNC 7112
MIM 607754
UniProt Q9UHC7  
 MKRN1 identified in exosomes derived from the following tissue/cell type
Prostate cancer cells 25844599    
 Gene ontology annotations for MKRN1
Molecular Function
    poly(A) RNA binding GO:0044822 IDA
    chromatin binding GO:0003682 IEA
    zinc ion binding GO:0008270 IEA
    ligase activity GO:0016874 IEA
    protein binding GO:0005515 IPI
    ubiquitin-protein transferase activity GO:0004842 IDA
Biological Process
    protein polyubiquitination GO:0000209 IDA
Subcellular Localization
    cellular_component GO:0005575 ND
 Experiment description of studies that identified MKRN1 in exosomes
1
Experiment ID 274
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
EV Enriched markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for MKRN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EXOSC8  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which MKRN1 is involved
PathwayEvidenceSource
Antigen processing: Ubiquitination & Proteasome degradation TAS Reactome





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