Gene description for MKRN1
Gene name makorin ring finger protein 1
Gene symbol MKRN1
Other names/aliases RNF61
Species Homo sapiens
 Database cross references - MKRN1
ExoCarta ExoCarta_23608
Vesiclepedia VP_23608
Entrez Gene 23608
HGNC 7112
MIM 607754
UniProt Q9UHC7  
 MKRN1 identified in exosomes derived from the following tissue/cell type
Prostate cancer cells 25844599    
 Gene ontology annotations for MKRN1
Molecular Function
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 IDA
    ubiquitin protein ligase activity GO:0061630 TAS
Biological Process
    protein polyubiquitination GO:0000209 IDA
    protein ubiquitination GO:0016567 IBA
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified MKRN1 in exosomes
1
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for MKRN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Matr3 17184
Affinity Capture-MS Mus musculus
2 NUPL2 11097
Affinity Capture-MS Homo sapiens
3 LARP1 23367
Affinity Capture-MS Homo sapiens
4 IGF2BP1 10642
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 Larp4  
Affinity Capture-MS Mus musculus
6 QARS 5859
Affinity Capture-MS Homo sapiens
7 ZNF346  
Affinity Capture-MS Homo sapiens
8 KIAA0020 9933
Affinity Capture-MS Homo sapiens
9 PABPC5 140886
Affinity Capture-MS Homo sapiens
10 Ybx1 22608
Affinity Capture-MS Mus musculus
11 APC  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
12 SF3B1 23451
Affinity Capture-MS Homo sapiens
13 GAPDHS 26330
Affinity Capture-MS Homo sapiens
14 BYSL 705
Two-hybrid Homo sapiens
15 Hnrnpu 51810
Affinity Capture-MS Mus musculus
16 KARS 3735
Affinity Capture-MS Homo sapiens
17 HNRNPCL1 343069
Affinity Capture-MS Homo sapiens
18 RPF2 84154
Affinity Capture-MS Homo sapiens
19 Caprin1 53872
Affinity Capture-MS Mus musculus
20 HNRNPUL2 221092
Affinity Capture-MS Homo sapiens
21 MAP1LC3B 81631
Two-hybrid Homo sapiens
22 Hnrnpd 11991
Affinity Capture-MS Mus musculus
23 Hnrnpa1 15382
Affinity Capture-MS Mus musculus
24 TOP2A 7153
Affinity Capture-MS Homo sapiens
25 IGF2BP3 10643
Affinity Capture-MS Homo sapiens
26 SNRNP70 6625
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 Ddx3x 13205
Affinity Capture-MS Mus musculus
28 DARS 1615
Affinity Capture-MS Homo sapiens
29 Elavl1 15568
Affinity Capture-MS Mus musculus
30 MTURN  
Two-hybrid Homo sapiens
31 DDX24 57062
Affinity Capture-MS Homo sapiens
32 Fam98a  
Affinity Capture-MS Mus musculus
33 THRAP3 9967
Affinity Capture-MS Homo sapiens
34 MDC1  
Affinity Capture-MS Homo sapiens
35 KHDRBS1 10657
Affinity Capture-MS Homo sapiens
36 KRI1  
Affinity Capture-MS Homo sapiens
37 Larp1  
Affinity Capture-MS Mus musculus
38 SRSF3 6428
Affinity Capture-MS Homo sapiens
39 TERT  
Affinity Capture-Western Homo sapiens
40 Fxr1 14359
Affinity Capture-MS Mus musculus
41 UBE2W  
Two-hybrid Homo sapiens
42 NQO1 1728
Affinity Capture-MS Homo sapiens
43 MYCN  
Affinity Capture-MS Homo sapiens
44 Mov10 17454
Affinity Capture-MS Mus musculus
45 SDCBP 6386
Two-hybrid Homo sapiens
46 Igf2bp3 140488
Affinity Capture-MS Mus musculus
47 PABPC4 8761
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 FAM120A 23196
Affinity Capture-MS Homo sapiens
49 RAD23A 5886
Two-hybrid Homo sapiens
50 Ilf2 67781
Affinity Capture-MS Mus musculus
51 G3bp2  
Affinity Capture-MS Mus musculus
52 Larp4b 217980
Affinity Capture-MS Mus musculus
53 BRIX1 55299
Affinity Capture-MS Homo sapiens
54 MRPL13  
Affinity Capture-MS Homo sapiens
55 RAD23B 5887
Two-hybrid Homo sapiens
56 CELF3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 PRPF8 10594
Affinity Capture-MS Homo sapiens
58 LIN28B  
Affinity Capture-MS Homo sapiens
59 CDKN1A  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
60 Pabpn1 54196
Affinity Capture-MS Mus musculus
61 DRG1 4733
Affinity Capture-MS Homo sapiens
62 TRA2A 29896
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 YBX2 51087
Affinity Capture-MS Homo sapiens
64 Lin28a  
Affinity Capture-MS Mus musculus
65 PURG  
Affinity Capture-MS Homo sapiens
66 Pabpc1 18458
Affinity Capture-MS Mus musculus
67 RPL5 6125
Affinity Capture-MS Homo sapiens
68 PABPC1 26986
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
69 MKRN2 23609
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 CKAP5 9793
Affinity Capture-MS Homo sapiens
71 Ddx1 104721
Affinity Capture-MS Mus musculus
72 LUC7L2 51631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 Dhx9 13211
Affinity Capture-MS Mus musculus
74 Hnrnpf 98758
Affinity Capture-MS Mus musculus
75 SMC1A 8243
Affinity Capture-MS Homo sapiens
76 UBE2D3 7323
Two-hybrid Homo sapiens
77 IARS 3376
Affinity Capture-MS Homo sapiens
78 RRP1B 23076
Affinity Capture-MS Homo sapiens
79 SON 6651
Affinity Capture-MS Homo sapiens
80 Atxn2l 233871
Affinity Capture-MS Mus musculus
81 DUSP23 54935
Two-hybrid Homo sapiens
82 GLTSCR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 YOD1  
Two-hybrid Homo sapiens
84 C11orf31  
Affinity Capture-MS Homo sapiens
85 Nufip2  
Affinity Capture-MS Mus musculus
86 RSL1D1 26156
Affinity Capture-MS Homo sapiens
87 PAIP2B  
Affinity Capture-MS Homo sapiens
88 SFPQ 6421
Affinity Capture-MS Homo sapiens
89 SMCHD1 23347
Affinity Capture-MS Homo sapiens
90 Atad3a  
Affinity Capture-MS Mus musculus
91 SMARCA5 8467
Affinity Capture-MS Homo sapiens
92 Igf2bp1  
Affinity Capture-MS Mus musculus
93 NAT10 55226
Affinity Capture-MS Homo sapiens
94 2700060E02Rik 68045
Affinity Capture-MS Mus musculus
95 Fip1l1  
Affinity Capture-MS Mus musculus
96 PHIP 55023
Affinity Capture-MS Homo sapiens
97 RPL3 6122
Affinity Capture-MS Homo sapiens
98 RBFOX1 54715
Affinity Capture-MS Homo sapiens
99 Hnrnpab 15384
Affinity Capture-MS Mus musculus
100 DHX30 22907
Affinity Capture-MS Homo sapiens
101 EPRS 2058
Affinity Capture-MS Homo sapiens
102 CSNK1G1 53944
Two-hybrid Homo sapiens
103 LARS 51520
Affinity Capture-MS Homo sapiens
104 Rbmx  
Affinity Capture-MS Mus musculus
105 MKRN1 23608
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
106 PRR3  
Affinity Capture-MS Homo sapiens
107 Casc3  
Affinity Capture-MS Mus musculus
108 Ago2 239528
Affinity Capture-MS Mus musculus
109 PRPF19 27339
Affinity Capture-MS Homo sapiens
110 MYC  
Affinity Capture-MS Homo sapiens
111 UBASH3A 53347
Two-hybrid Homo sapiens
112 Hnrnpa3 229279
Affinity Capture-MS Mus musculus
113 L1td1  
Affinity Capture-MS Mus musculus
114 Syncrip 56403
Affinity Capture-MS Mus musculus
115 Eif4g1 208643
Affinity Capture-MS Mus musculus
116 KCNH1 3756
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
117 Usp10  
Affinity Capture-MS Mus musculus
118 Srrt 83701
Affinity Capture-MS Mus musculus
119 RBMS2 5939
Affinity Capture-MS Homo sapiens
120 NAA10 8260
Two-hybrid Homo sapiens
121 HUWE1 10075
Affinity Capture-MS Homo sapiens
122 AIMP1 9255
Affinity Capture-MS Homo sapiens
123 PAIP1 10605
Affinity Capture-MS Homo sapiens
124 SUPT16H 11198
Affinity Capture-MS Homo sapiens
125 YBX1 4904
Affinity Capture-MS Homo sapiens
126 FADD 8772
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
127 Upf1 19704
Affinity Capture-MS Mus musculus
128 BCAS2 10286
Affinity Capture-MS Homo sapiens
129 Hadha  
Affinity Capture-MS Mus musculus
130 TP53 7157
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
131 RRS1 23212
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
132 PES1 23481
Affinity Capture-MS Homo sapiens
133 APOBEC3D  
Affinity Capture-MS Homo sapiens
134 MKRN3  
Affinity Capture-MS Homo sapiens
135 ELAVL1 1994
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
136 AXIN1  
Affinity Capture-Western Homo sapiens
137 MKI67  
Affinity Capture-MS Homo sapiens
138 Hnrnpl 15388
Affinity Capture-MS Mus musculus
139 Cpsf6  
Affinity Capture-MS Mus musculus
140 SMC3 9126
Affinity Capture-MS Homo sapiens
141 SNIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
142 GSPT2 23708
Affinity Capture-MS Homo sapiens
143 HNRNPC 3183
Affinity Capture-MS Homo sapiens
144 FAM161A  
Two-hybrid Homo sapiens
145 DAXX  
Two-hybrid Homo sapiens
146 EXOSC8  
Two-hybrid Homo sapiens
147 Mkrn1 54484
Affinity Capture-MS Mus musculus
148 RARS 5917
Affinity Capture-MS Homo sapiens
149 Prrc2c 226562
Affinity Capture-MS Mus musculus
150 G3bp1  
Affinity Capture-MS Mus musculus
151 LUC7L 55692
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
152 Hnrnpa2b1 53379
Affinity Capture-MS Mus musculus
153 CDC5L 988
Affinity Capture-MS Homo sapiens
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