Gene description for Hnrnpa1
Gene name heterogeneous nuclear ribonucleoprotein A1
Gene symbol Hnrnpa1
Other names/aliases HDP-1
Hdp
Hnrpa1
hnRNP A1
hnrnp-A1
Species Mus musculus
 Database cross references - Hnrnpa1
ExoCarta ExoCarta_15382
Vesiclepedia VP_15382
Entrez Gene 15382
UniProt P49312  
 Hnrnpa1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hnrnpa1
Molecular Function
    DNA binding GO:0003677 ISO
    single-stranded DNA binding GO:0003697 ISO
    single-stranded DNA binding GO:0003697 ISS
    RNA binding GO:0003723 ISO
    single-stranded RNA binding GO:0003727 ISO
    mRNA binding GO:0003729 ISO
    mRNA 3'-UTR binding GO:0003730 IBA
    mRNA 3'-UTR binding GO:0003730 ISO
    protein domain specific binding GO:0019904 ISO
    RNA strand annealing activity GO:0033592 ISO
    miRNA binding GO:0035198 ISO
    miRNA binding GO:0035198 ISS
    pre-mRNA binding GO:0036002 ISO
    identical protein binding GO:0042802 ISO
    telomeric repeat-containing RNA binding GO:0061752 ISO
    G-rich strand telomeric DNA binding GO:0098505 ISO
    DNA/DNA annealing activity GO:1990814 ISO
    sequence-specific mRNA binding GO:1990825 ISO
Biological Process
    alternative mRNA splicing, via spliceosome GO:0000380 IDA
    mRNA splicing, via spliceosome GO:0000398 IBA
    RNA splicing GO:0008380 IDA
    positive regulation of gene expression GO:0010628 ISO
    cellular response to potassium ion GO:0035865 ISO
    positive regulation of action potential GO:0045760 ISO
    mRNA transport GO:0051028 IEA
    nuclear export GO:0051168 ISS
    import into nucleus GO:0051170 ISS
    cellular response to sodium arsenite GO:1903936 ISS
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 ISS
    spliceosomal complex GO:0005681 ISO
    spliceosomal complex GO:0005681 ISS
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    catalytic step 2 spliceosome GO:0071013 IBA
    catalytic step 2 spliceosome GO:0071013 ISO
    nucleoplasmic periphery of the nuclear pore complex GO:1990826 ISO
    ribonucleoprotein complex GO:1990904 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Hnrnpa1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hnrnpa1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Npm1 18148
Affinity Capture-MS Mus musculus
2 Cbx5  
Affinity Capture-MS Mus musculus
3 Cbx1  
Affinity Capture-MS Mus musculus
4 Pard3b  
Two-hybrid Mus musculus
5 Tmem173  
Proximity Label-MS Mus musculus
6 Fzr1  
Affinity Capture-MS Mus musculus
7 Eed  
Affinity Capture-MS Mus musculus
8 MKRN1 23608
Affinity Capture-MS Homo sapiens
9 Tfe3  
Affinity Capture-MS Mus musculus
10 Atg16l1  
Affinity Capture-MS Mus musculus
11 Dmrt2  
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
12 Srsf9  
Two-hybrid Mus musculus
13 Kctd13  
Affinity Capture-MS Mus musculus
14 Cbx3 12417
Affinity Capture-MS Mus musculus
15 ERP29 10961
Two-hybrid Homo sapiens
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