Gene description for Ddx1
Gene name DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
Gene symbol Ddx1
Other names/aliases AA409185
DBP-RB
Species Mus musculus
 Database cross references - Ddx1
ExoCarta ExoCarta_104721
Vesiclepedia VP_104721
Entrez Gene 104721
UniProt Q91VR5  
 Ddx1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ddx1
Molecular Function
    helicase activity GO:0004386 IEA
    nuclease activity GO:0004518 ISO
    double-stranded RNA binding GO:0003725 IDA
    protein binding GO:0005515 IPI
    DNA/RNA helicase activity GO:0033677 ISO
    hydrolase activity GO:0016787 IEA
    nucleic acid binding GO:0003676 IEA
    poly(A) RNA binding GO:0044822 ISO
    exonuclease activity GO:0004527 IEA
    poly(A) binding GO:0008143 ISO
    DNA binding GO:0003677 IEA
    ATP-dependent RNA helicase activity GO:0004004 IBA
    transcription cofactor activity GO:0003712 ISO
    RNA binding GO:0003723 IEA
    chromatin binding GO:0003682 ISO
    ATP-dependent helicase activity GO:0008026 ISO
    nucleotide binding GO:0000166 IEA
    ATP binding GO:0005524 IEA
Biological Process
    tRNA processing GO:0008033 IEA
    regulation of transcription, DNA-templated GO:0006355 IEA
    tRNA splicing, via endonucleolytic cleavage and ligation GO:0006388 ISO
    nucleic acid phosphodiester bond hydrolysis GO:0090305 ISO
    response to virus GO:0009615 IMP
    regulation of nucleic acid-templated transcription GO:1903506 ISO
    response to exogenous dsRNA GO:0043330 IMP
    transcription, DNA-templated GO:0006351 IEA
    mRNA processing GO:0006397 IEA
    DNA duplex unwinding GO:0032508 ISO
    double-strand break repair GO:0006302 ISO
    RNA secondary structure unwinding GO:0010501 IBA
Subcellular Localization
    cytoplasmic stress granule GO:0010494 ISO
    ribonucleoprotein complex GO:0030529 ISO
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    nucleus GO:0005634 ISO
    tRNA-splicing ligase complex GO:0072669 ISO
    membrane GO:0016020 ISO
    cleavage body GO:0071920 ISO
 Experiment description of studies that identified Ddx1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ddx1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ddx1 is involved
No pathways found





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