Gene description for 2700060E02Rik
Gene name RIKEN cDNA 2700060E02 gene
Gene symbol 2700060E02Rik
Other names/aliases -
Species Mus musculus
 Database cross references - 2700060E02Rik
ExoCarta ExoCarta_68045
Vesiclepedia VP_68045
Entrez Gene 68045
UniProt Q9CQE8  
 2700060E02Rik identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for 2700060E02Rik
Molecular Function
    RNA polymerase II complex binding GO:0000993 ISO
    RNA polymerase II complex binding GO:0000993 ISS
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 ISO
    RNA binding GO:0003723 ISS
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    tRNA splicing, via endonucleolytic cleavage and ligation GO:0006388 IBA
    tRNA splicing, via endonucleolytic cleavage and ligation GO:0006388 ISO
    tRNA splicing, via endonucleolytic cleavage and ligation GO:0006388 ISS
    negative regulation of protein kinase activity GO:0006469 ISO
    negative regulation of protein kinase activity GO:0006469 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    centrosome GO:0005813 ISO
    centrosome GO:0005813 ISS
    cytosol GO:0005829 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    perinuclear region of cytoplasm GO:0048471 ISS
    tRNA-splicing ligase complex GO:0072669 IBA
    tRNA-splicing ligase complex GO:0072669 ISO
    tRNA-splicing ligase complex GO:0072669 ISS
    mitotic spindle GO:0072686 ISO
    mitotic spindle GO:0072686 ISS
 Experiment description of studies that identified 2700060E02Rik in exosomes
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for 2700060E02Rik
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 D2Wsu81e  
Two-hybrid Mus musculus
2 Mrpl13  
Two-hybrid Mus musculus
3 MKRN1 23608
Affinity Capture-MS Homo sapiens
4 Nmt1  
Co-fractionation Mus musculus
5 DGCR14  
Two-hybrid Homo sapiens
6 FAM98A 25940
Two-hybrid Homo sapiens
7 BCAS2 10286
Two-hybrid Homo sapiens
8 Fkbp3  
Co-fractionation Mus musculus
9 Abt1  
Two-hybrid Mus musculus
10 Rtcb 28088
Co-fractionation Mus musculus
11 PRPF6 24148
Two-hybrid Homo sapiens
12 Kctd13  
Affinity Capture-MS Mus musculus
13 Hnrnpu 51810
Co-fractionation Mus musculus
14 KPNA2 3838
Two-hybrid Homo sapiens
15 RPP30 10556
Two-hybrid Homo sapiens
16 NXF1 10482
Two-hybrid Homo sapiens
17 Tmem173  
Proximity Label-MS Mus musculus
18 TRNAU1AP  
Two-hybrid Homo sapiens
19 Syncrip 56403
Co-fractionation Mus musculus
20 Fez2  
Two-hybrid Mus musculus
21 Fam98a  
Co-fractionation Mus musculus
22 ERP29 10961
Two-hybrid Homo sapiens
23 Ddx1 104721
Co-fractionation Mus musculus
24 Zbtb7b  
Affinity Capture-MS Mus musculus
25 PRC1 9055
Two-hybrid Homo sapiens
26 Fam98b 68215
Co-fractionation Mus musculus
27 PCM1 5108
Two-hybrid Homo sapiens
28 Krt18 16668
Co-fractionation Mus musculus
29 Otx2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which 2700060E02Rik is involved
No pathways found





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