Gene description for RPP30
Gene name ribonuclease P/MRP 30kDa subunit
Gene symbol RPP30
Other names/aliases TSG15
Species Homo sapiens
 Database cross references - RPP30
ExoCarta ExoCarta_10556
Vesiclepedia VP_10556
Entrez Gene 10556
HGNC 17688
MIM 606115
UniProt P78346  
 RPP30 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for RPP30
Molecular Function
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    ribonuclease P activity GO:0004526 IDA
    protein binding GO:0005515 IPI
    ribonuclease P RNA binding GO:0033204 IDA
Biological Process
    tRNA 5'-leader removal GO:0001682 IDA
    rRNA processing GO:0006364 IEA
    tRNA processing GO:0008033 IBA
Subcellular Localization
    ribonuclease MRP complex GO:0000172 IDA
    nucleus GO:0005634 TAS
    nucleoplasm GO:0005654 TAS
    nucleolar ribonuclease P complex GO:0005655 IBA
    multimeric ribonuclease P complex GO:0030681 IDA
 Experiment description of studies that identified RPP30 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RPP30
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KIF15 56992
Co-fractionation Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 PSMA6 5687
Co-fractionation Homo sapiens
4 HEATR6  
Co-fractionation Homo sapiens
5 RIT1 6016
Negative Genetic Homo sapiens
6 POP7  
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 VWA8 23078
Affinity Capture-MS Homo sapiens
8 SNX27 81609
Affinity Capture-MS Homo sapiens
9 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
10 Eif1b  
Two-hybrid Mus musculus
11 SIRT6  
Affinity Capture-MS Homo sapiens
12 RPP40 10799
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 C18orf21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 DNAJC8 22826
Proximity Label-MS Homo sapiens
15 FBXL6  
Affinity Capture-MS Homo sapiens
16 EGFR 1956
Negative Genetic Homo sapiens
17 NPM3 10360
Affinity Capture-MS Homo sapiens
18 POP1 10940
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 INPPL1 3636
Co-fractionation Homo sapiens
20 MNDA 4332
Affinity Capture-MS Homo sapiens
21 SSB 6741
Affinity Capture-MS Homo sapiens
22 POP5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
23 PTPN6 5777
Affinity Capture-MS Homo sapiens
24 2700060E02Rik 68045
Two-hybrid Mus musculus
25 RPP25L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 MLF2 8079
Co-fractionation Homo sapiens
27 ACTC1 70
Proximity Label-MS Homo sapiens
28 RPP14  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
29 POP4 10775
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
30 INTS2  
Co-fractionation Homo sapiens
31 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 CBWD2  
Affinity Capture-MS Homo sapiens
33 RNF32  
Two-hybrid Homo sapiens
34 MAST1  
Two-hybrid Homo sapiens
35 PTPN2 5771
Affinity Capture-MS Homo sapiens
36 CAND1 55832
Affinity Capture-MS Homo sapiens
37 KHDRBS2  
Two-hybrid Homo sapiens
38 TRIM39-RPP21  
Affinity Capture-MS Homo sapiens
39 RBM4B  
Affinity Capture-MS Homo sapiens
40 LRRC16A 55604
Co-fractionation Homo sapiens
41 PSMD14 10213
Affinity Capture-MS Homo sapiens
42 DNAJB6 10049
Proximity Label-MS Homo sapiens
43 PSMB1 5689
Co-fractionation Homo sapiens
44 UFL1 23376
Affinity Capture-MS Homo sapiens
45 MYCN  
Affinity Capture-MS Homo sapiens
46 RPA3 6119
Proximity Label-MS Homo sapiens
47 CEBPA  
Protein-peptide Homo sapiens
48 CALU 813
Co-fractionation Homo sapiens
49 USP36  
Affinity Capture-MS Homo sapiens
50 BTLA  
Affinity Capture-MS Homo sapiens
51 RPP25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 MYC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 ARL1 400
Affinity Capture-MS Homo sapiens
54 BARD1 580
Affinity Capture-MS Homo sapiens
55 C16orf72 29035
Affinity Capture-MS Homo sapiens
56 HECTD1 25831
Affinity Capture-MS Homo sapiens
57 DNAJC9 23234
Proximity Label-MS Homo sapiens
58 MAGEA4 4103
Affinity Capture-MS Homo sapiens
59 IFI16 3428
Affinity Capture-MS Homo sapiens
60 CIT 11113
Affinity Capture-MS Homo sapiens
61 CBX6  
Affinity Capture-MS Homo sapiens
62 RPP21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 ENOX2  
Affinity Capture-MS Homo sapiens
64 DDRGK1 65992
Affinity Capture-MS Homo sapiens
65 THTPA  
Affinity Capture-MS Homo sapiens
66 C9orf72  
Affinity Capture-MS Homo sapiens
67 WDR44 54521
Affinity Capture-MS Homo sapiens
68 NPM1 4869
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 SMYD2 56950
Affinity Capture-MS Homo sapiens
70 RPS16 6217
Affinity Capture-MS Homo sapiens
71 MEN1 4221
Affinity Capture-MS Homo sapiens
72 HMG20A  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
73 CBWD1 55871
Affinity Capture-MS Homo sapiens
74 SURF6  
Affinity Capture-MS Homo sapiens
75 RPP38 10557
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 EFNA2  
Affinity Capture-MS Homo sapiens
77 KRAS 3845
Negative Genetic Homo sapiens
78 HUWE1 10075
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 C3orf17  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 EPB41L1 2036
Co-fractionation Homo sapiens
View the network image/svg+xml



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