Gene description for RPP40
Gene name ribonuclease P/MRP 40kDa subunit
Gene symbol RPP40
Other names/aliases RNASEP1
bA428J1.3
Species Homo sapiens
 Database cross references - RPP40
ExoCarta ExoCarta_10799
Vesiclepedia VP_10799
Entrez Gene 10799
HGNC 20992
MIM 606117
UniProt O75818  
 RPP40 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for RPP40
Molecular Function
    ribonuclease MRP activity GO:0000171 IBA
    ribonuclease P activity GO:0004526 IBA
    ribonuclease P activity GO:0004526 IDA
    protein binding GO:0005515 IPI
    ribonuclease P RNA binding GO:0033204 IDA
Biological Process
    endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000447 IBA
    tRNA 5'-leader removal GO:0001682 IBA
    tRNA 5'-leader removal GO:0001682 IDA
Subcellular Localization
    ribonuclease MRP complex GO:0000172 IBA
    nucleus GO:0005634 TAS
    nucleoplasm GO:0005654 TAS
    nucleolar ribonuclease P complex GO:0005655 TAS
    multimeric ribonuclease P complex GO:0030681 IBA
    multimeric ribonuclease P complex GO:0030681 IDA
 Experiment description of studies that identified RPP40 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RPP40
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RALY 22913
Co-fractionation Homo sapiens
2 NPM3 10360
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 POP7  
Co-purification Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
5 MECP2 4204
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 PMS2P1  
Co-fractionation Homo sapiens
8 C18orf21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 FBXL6  
Affinity Capture-MS Homo sapiens
10 POP1 10940
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
11 RPP25L  
Affinity Capture-MS Homo sapiens
12 COPG2 26958
Affinity Capture-MS Homo sapiens
13 VTN 7448
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 POP5  
Affinity Capture-MS Homo sapiens
15 RPL13A 23521
Affinity Capture-MS Homo sapiens
16 RPP14  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
17 POP4 10775
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
18 SSB 6741
Affinity Capture-MS Homo sapiens
19 RPP30 10556
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
20 PDCD11 22984
Affinity Capture-MS Homo sapiens
21 RBM4B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 IGFBP5 3488
Affinity Capture-MS Homo sapiens
23 RBM4 5936
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 AKR7L 246181
Affinity Capture-MS Homo sapiens
25 CBX6  
Affinity Capture-MS Homo sapiens
26 SNRNP70 6625
Affinity Capture-MS Homo sapiens
27 RPP25  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
28 IFI16 3428
Affinity Capture-MS Homo sapiens
29 MEOX2  
Two-hybrid Homo sapiens
30 RFC3 5983
Co-fractionation Homo sapiens
31 RPP21  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 DDRGK1 65992
Affinity Capture-MS Homo sapiens
33 SNRPC 6631
Affinity Capture-MS Homo sapiens
34 NPM1 4869
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 RPP38 10557
Co-fractionation Homo sapiens
36 GNB2L1 10399
Affinity Capture-MS Homo sapiens
37 C3orf17  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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