Gene description for KIF15
Gene name kinesin family member 15
Gene symbol KIF15
Other names/aliases HKLP2
KNSL7
NY-BR-62
Species Homo sapiens
 Database cross references - KIF15
ExoCarta ExoCarta_56992
Entrez Gene 56992
HGNC 17273
UniProt Q9NS87  
 KIF15 identified in exosomes derived from the following tissue/cell type
Mesothelioma cells 15111327    
Prostate cancer cells 25844599    
Thymus 23844026    
 Gene ontology annotations for KIF15
Molecular Function
    ATPase activity GO:0016887 IBA
    microtubule motor activity GO:0003777 IBA
    microtubule binding GO:0008017 IEA
    motor activity GO:0003774 TAS
    DNA binding GO:0003677 TAS
    ATP binding GO:0005524 IEA
Biological Process
    metabolic process GO:0008152 TAS
    blood coagulation GO:0007596 TAS
    mitotic nuclear division GO:0007067 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 TAS
    microtubule-based movement GO:0007018 TAS
    cell proliferation GO:0008283 TAS
Subcellular Localization
    plus-end kinesin complex GO:0005873 TAS
    spindle GO:0005819 IEA
    membrane GO:0016020 IDA
    microtubule GO:0005874 IEA
    centrosome GO:0005813 TAS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified KIF15 in exosomes
1
Experiment ID 25
ISEV standards
✔
IEM
EV Biophysical techniques
✔
HSP90|beta-tubulin
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15111327    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes secreted by human mesothelioma cells.
Authors Hegmans JP, Bard MP, Hemmes A, Luider TM, Kleijmeer MJ, Prins JB, Zitvogel L, Burgers SA, Hoogsteden HC, Lambrecht BN.
Journal name AJP
Publication year 2004
Sample Mesothelioma cells
Sample name PMR-MM7
PMR-MM8
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
2
Experiment ID 275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
3
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KIF15
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CHD3  
Two-hybrid Homo sapiens
2 VIM 7431
Two-hybrid Homo sapiens
3 MAP1LC3A  
Two-hybrid Homo sapiens
4 MKI67  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
5 KIAA1377  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which KIF15 is involved
PathwayEvidenceSource
Kinesins TAS Reactome
MHC class II antigen presentation TAS Reactome





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