Gene description for KIF15
Gene name kinesin family member 15
Gene symbol KIF15
Other names/aliases HKLP2
KNSL7
NY-BR-62
Species Homo sapiens
 Database cross references - KIF15
ExoCarta ExoCarta_56992
Vesiclepedia VP_56992
Entrez Gene 56992
HGNC 17273
UniProt Q9NS87  
 KIF15 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Mesothelioma cells 15111327    
Prostate cancer cells 25844599    
Thymus 23844026    
 Gene ontology annotations for KIF15
Molecular Function
    cytoskeletal motor activity GO:0003774 TAS
    microtubule motor activity GO:0003777 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    microtubule binding GO:0008017 IBA
    plus-end-directed microtubule motor activity GO:0008574 ISS
    ATP hydrolysis activity GO:0016887 IBA
Biological Process
    mitotic cell cycle GO:0000278 TAS
    microtubule-based movement GO:0007018 IBA
    centrosome separation GO:0051299 ISS
    mitotic spindle assembly GO:0090307 ISS
Subcellular Localization
    spindle pole GO:0000922 ISS
    cytoplasm GO:0005737 IBA
    centrosome GO:0005813 TAS
    cytosol GO:0005829 TAS
    kinesin complex GO:0005871 IBA
    plus-end kinesin complex GO:0005873 TAS
    microtubule GO:0005874 IBA
    membrane GO:0016020 HDA
 Experiment description of studies that identified KIF15 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 25
MISEV standards
✔
IEM
Biophysical techniques
✔
HSP90|beta-tubulin
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15111327    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes secreted by human mesothelioma cells.
Authors "Hegmans JP, Bard MP, Hemmes A, Luider TM, Kleijmeer MJ, Prins JB, Zitvogel L, Burgers SA, Hoogsteden HC, Lambrecht BN."
Journal name AJP
Publication year 2004
Sample Mesothelioma cells
Sample name PMR-MM7
PMR-MM8
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
7
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KIF15
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ELMO3 79767
Affinity Capture-MS Homo sapiens
2 PISD  
Affinity Capture-MS Homo sapiens
3 KRT18 3875
Proximity Label-MS Homo sapiens
4 PHLPP1  
Proximity Label-MS Homo sapiens
5 USP10 9100
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
6 MRPL44  
Affinity Capture-MS Homo sapiens
7 ANKFY1 51479
Proximity Label-MS Homo sapiens
8 CHD3 1107
Two-hybrid Homo sapiens
9 CLIP1 6249
Proximity Label-MS Homo sapiens
10 AMOT  
Proximity Label-MS Homo sapiens
11 CANX 821
Cross-Linking-MS (XL-MS) Homo sapiens
12 PGK1 5230
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
13 INPPL1 3636
Co-fractionation Homo sapiens
14 MCC 4163
Affinity Capture-MS Homo sapiens
15 PFN1 5216
Proximity Label-MS Homo sapiens
16 PPM1B 5495
Proximity Label-MS Homo sapiens
17 KRT8 3856
Proximity Label-MS Homo sapiens
18 STAM2 10254
Co-fractionation Homo sapiens
19 EZR 7430
Proximity Label-MS Homo sapiens
20 MRI1 84245
Affinity Capture-MS Homo sapiens
21 CD2AP 23607
Affinity Capture-MS Homo sapiens
22 DGCR8  
Affinity Capture-MS Homo sapiens
23 ISCA1  
Affinity Capture-MS Homo sapiens
24 DEK 7913
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 SEPT10 151011
Proximity Label-MS Homo sapiens
26 DCTN2 10540
Proximity Label-MS Homo sapiens
27 RPP30 10556
Co-fractionation Homo sapiens
28 HNRNPA1 3178
Cross-Linking-MS (XL-MS) Homo sapiens
29 KIAA1377  
Two-hybrid Homo sapiens
30 CLTB 1212
Proximity Label-MS Homo sapiens
31 AXIN2 8313
Affinity Capture-MS Homo sapiens
32 MKI67  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
33 CUL4B 8450
Affinity Capture-MS Homo sapiens
34 GORASP1 64689
Proximity Label-MS Homo sapiens
35 MED6  
Affinity Capture-MS Homo sapiens
36 MPC1 51660
Affinity Capture-MS Homo sapiens
37 ANAPC2 29882
Proximity Label-MS Homo sapiens
38 LRPPRC 10128
Co-fractionation Homo sapiens
39 PEX14 5195
Proximity Label-MS Homo sapiens
40 MAPRE3  
Proximity Label-MS Homo sapiens
41 MAP1LC3A 84557
Two-hybrid Homo sapiens
42 KDM1A 23028
Proximity Label-MS Homo sapiens
43 PHYHIP 9796
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 KIF1BP 26128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 MYC  
Affinity Capture-MS Homo sapiens
46 JAK1 3716
Affinity Capture-MS Homo sapiens
47 NTRK1 4914
Affinity Capture-MS Homo sapiens
48 SYNC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 CLTA 1211
Proximity Label-MS Homo sapiens
50 KXD1 79036
Affinity Capture-MS Homo sapiens
51 NDC80 10403
Proximity Label-MS Homo sapiens
52 KIAA1143  
Affinity Capture-MS Homo sapiens
53 IMPDH2 3615
Proximity Label-MS Homo sapiens
54 LRBA 987
Co-fractionation Homo sapiens
55 PTGES3 10728
Affinity Capture-MS Homo sapiens
56 PCM1 5108
Proximity Label-MS Homo sapiens
57 FANCG 2189
Affinity Capture-MS Homo sapiens
58 DMD 1756
Affinity Capture-MS Homo sapiens
59 VIM 7431
Two-hybrid Homo sapiens
60 KRT19 3880
Proximity Label-MS Homo sapiens
61 MLF2 8079
Co-fractionation Homo sapiens
62 UPF2 26019
Affinity Capture-MS Homo sapiens
63 MPDZ  
Affinity Capture-MS Homo sapiens
64 FAM9C  
Affinity Capture-MS Homo sapiens
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