Gene description for Mkrn1
Gene name makorin, ring finger protein, 1
Gene symbol Mkrn1
Other names/aliases RFP
Species Mus musculus
 Database cross references - Mkrn1
ExoCarta ExoCarta_54484
Vesiclepedia VP_54484
Entrez Gene 54484
 Mkrn1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Mkrn1
Molecular Function
    protein-glycine ligase activity, initiating GO:0070736 IEA
    metal ion binding GO:0046872 IEA
    tubulin-glycine ligase activity GO:0070738 IEA
    tubulin-glutamic acid ligase activity GO:0070740 IEA
    protein-glutamic acid ligase activity GO:0070739 IEA
    ligase activity GO:0016874 IEA
    UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity GO:0008766 IEA
    chromatin binding GO:0003682 IDA
    ubiquitin-protein transferase activity GO:0004842 ISO
    protein-glycine ligase activity GO:0070735 IEA
    coenzyme F420-2 alpha-glutamyl ligase activity GO:0043774 IEA
    zinc ion binding GO:0008270 IEA
    poly(A) RNA binding GO:0044822 ISO
    protein-glycine ligase activity, elongating GO:0070737 IEA
    coenzyme F420-0 gamma-glutamyl ligase activity GO:0043773 IEA
    ribosomal S6-glutamic acid ligase activity GO:0018169 IEA
Biological Process
    protein polyubiquitination GO:0000209 ISO
 Experiment description of studies that identified Mkrn1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mkrn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Mkrn1 is involved
No pathways found





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