Gene description for Ddx3x
Gene name DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked
Gene symbol Ddx3x
Other names/aliases D1Pas1-rs2
Ddx3
Fin14
Species Mus musculus
 Database cross references - Ddx3x
ExoCarta ExoCarta_13205
Vesiclepedia VP_13205
Entrez Gene 13205
UniProt Q62167  
 Ddx3x identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ddx3x
Molecular Function
    DNA binding GO:0003677 ISO
    DNA binding GO:0003677 ISS
    DNA helicase activity GO:0003678 ISO
    DNA helicase activity GO:0003678 ISS
    DNA clamp loader activity GO:0003689 IEA
    RNA binding GO:0003723 ISO
    RNA binding GO:0003723 ISS
    RNA helicase activity GO:0003724 IBA
    RNA helicase activity GO:0003724 ISO
    RNA helicase activity GO:0003724 ISS
    mRNA binding GO:0003729 IBA
    mRNA binding GO:0003729 ISO
    mRNA binding GO:0003729 ISS
    GTPase activity GO:0003924 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    transcription factor binding GO:0008134 ISO
    transcription factor binding GO:0008134 ISS
    poly(A) binding GO:0008143 ISO
    poly(A) binding GO:0008143 ISS
    eukaryotic initiation factor 4E binding GO:0008190 ISO
    eukaryotic initiation factor 4E binding GO:0008190 ISS
    ATP hydrolysis activity GO:0016887 ISO
    ATP hydrolysis activity GO:0016887 ISS
    ribonucleoside triphosphate phosphatase activity GO:0017111 ISO
    translation initiation factor binding GO:0031369 ISO
    translation initiation factor binding GO:0031369 ISS
    RNA strand annealing activity GO:0033592 ISO
    signaling adaptor activity GO:0035591 ISO
    RNA stem-loop binding GO:0035613 ISO
    RNA stem-loop binding GO:0035613 ISS
    gamma-tubulin binding GO:0043015 ISO
    gamma-tubulin binding GO:0043015 ISS
    ribosomal small subunit binding GO:0043024 ISO
    ribosomal small subunit binding GO:0043024 ISS
    CTPase activity GO:0043273 ISO
    protein serine/threonine kinase activator activity GO:0043539 ISO
    protein serine/threonine kinase activator activity GO:0043539 ISS
    mRNA 5'-UTR binding GO:0048027 ISO
    mRNA 5'-UTR binding GO:0048027 ISS
    cohesin loader activity GO:0061775 IEA
    primary miRNA binding GO:0070878 IDA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    translational initiation GO:0006413 ISO
    inflammatory response GO:0006954 IEA
    chromosome segregation GO:0007059 ISO
    chromosome segregation GO:0007059 ISS
    gamete generation GO:0007276 IBA
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 ISO
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 ISS
    response to virus GO:0009615 ISO
    response to virus GO:0009615 ISS
    RNA secondary structure unwinding GO:0010501 ISO
    RNA secondary structure unwinding GO:0010501 ISS
    positive regulation of gene expression GO:0010628 ISO
    negative regulation of gene expression GO:0010629 IBA
    Wnt signaling pathway GO:0016055 ISO
    negative regulation of translation GO:0017148 ISO
    negative regulation of translation GO:0017148 ISS
    cell differentiation GO:0030154 IBA
    positive regulation of cell growth GO:0030307 ISO
    positive regulation of cell growth GO:0030307 ISS
    negative regulation of cell growth GO:0030308 ISO
    primary miRNA processing GO:0031053 IDA
    primary miRNA processing GO:0031053 IMP
    negative regulation of protein-containing complex assembly GO:0031333 ISO
    negative regulation of protein-containing complex assembly GO:0031333 ISS
    positive regulation of protein autophosphorylation GO:0031954 ISO
    positive regulation of protein autophosphorylation GO:0031954 ISS
    positive regulation of type I interferon production GO:0032481 ISO
    DNA duplex unwinding GO:0032508 IEA
    positive regulation of interferon-alpha production GO:0032727 ISO
    positive regulation of interferon-alpha production GO:0032727 ISS
    positive regulation of interferon-beta production GO:0032728 ISO
    positive regulation of interferon-beta production GO:0032728 ISS
    stress granule assembly GO:0034063 IMP
    stress granule assembly GO:0034063 ISO
    stress granule assembly GO:0034063 ISS
    positive regulation of toll-like receptor 7 signaling pathway GO:0034157 ISO
    positive regulation of toll-like receptor 7 signaling pathway GO:0034157 ISS
    positive regulation of toll-like receptor 8 signaling pathway GO:0034161 ISO
    positive regulation of toll-like receptor 8 signaling pathway GO:0034161 ISS
    intracellular signal transduction GO:0035556 ISO
    intracellular signal transduction GO:0035556 ISS
    positive regulation of translation in response to endoplasmic reticulum stress GO:0036493 ISO
    positive regulation of translation in response to endoplasmic reticulum stress GO:0036493 ISS
    cytosolic ribosome assembly GO:0042256 ISO
    cytosolic ribosome assembly GO:0042256 ISS
    positive regulation of apoptotic process GO:0043065 ISO
    positive regulation of apoptotic process GO:0043065 ISS
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of apoptotic process GO:0043066 ISS
    positive regulation of viral genome replication GO:0045070 ISO
    innate immune response GO:0045087 ISO
    innate immune response GO:0045087 ISS
    positive regulation of translation GO:0045727 ISO
    positive regulation of translation GO:0045727 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    positive regulation of translational initiation GO:0045948 ISO
    positive regulation of translational initiation GO:0045948 ISS
    lipid homeostasis GO:0055088 ISO
    lipid homeostasis GO:0055088 ISS
    cellular response to arsenic-containing substance GO:0071243 ISO
    cellular response to arsenic-containing substance GO:0071243 ISS
    cellular response to osmotic stress GO:0071470 ISO
    cellular response to osmotic stress GO:0071470 ISS
    positive regulation of protein serine/threonine kinase activity GO:0071902 ISO
    positive regulation of protein serine/threonine kinase activity GO:0071902 ISS
    positive regulation of canonical Wnt signaling pathway GO:0090263 ISO
    positive regulation of canonical Wnt signaling pathway GO:0090263 ISS
    intrinsic apoptotic signaling pathway GO:0097193 ISO
    intrinsic apoptotic signaling pathway GO:0097193 ISS
    cellular response to virus GO:0098586 ISO
    chromatin looping GO:0140588 IEA
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 ISO
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 ISS
    positive regulation of NLRP3 inflammasome complex assembly GO:1900227 IDA
    negative regulation of non-canonical NF-kappaB signal transduction GO:1901223 ISO
    negative regulation of non-canonical NF-kappaB signal transduction GO:1901223 ISS
    positive regulation of non-canonical NF-kappaB signal transduction GO:1901224 ISO
    positive regulation of non-canonical NF-kappaB signal transduction GO:1901224 ISS
    positive regulation of protein acetylation GO:1901985 ISO
    positive regulation of protein acetylation GO:1901985 ISS
    negative regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902042 ISO
    negative regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902042 ISS
    positive regulation of protein K63-linked ubiquitination GO:1902523 ISO
    positive regulation of protein K63-linked ubiquitination GO:1902523 ISS
    protein localization to cytoplasmic stress granule GO:1903608 ISO
    negative regulation of cysteine-type endopeptidase activity GO:2000117 ISO
    negative regulation of cysteine-type endopeptidase activity GO:2000117 ISS
    positive regulation of cysteine-type endopeptidase activity GO:2001056 ISO
    positive regulation of cysteine-type endopeptidase activity GO:2001056 ISS
    negative regulation of intrinsic apoptotic signaling pathway GO:2001243 ISO
    negative regulation of intrinsic apoptotic signaling pathway GO:2001243 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    centrosome GO:0005813 ISO
    centrosome GO:0005813 ISS
    cytosol GO:0005829 ISO
    eukaryotic translation initiation factor 3 complex GO:0005852 ISO
    eukaryotic translation initiation factor 3 complex GO:0005852 ISS
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    cytoplasmic stress granule GO:0010494 IDA
    cytoplasmic stress granule GO:0010494 ISO
    cytoplasmic stress granule GO:0010494 ISS
    postsynaptic density GO:0014069 IEA
    cytosolic small ribosomal subunit GO:0022627 ISO
    cytosolic small ribosomal subunit GO:0022627 ISS
    lamellipodium GO:0030027 IEA
    cell leading edge GO:0031252 ISO
    cell leading edge GO:0031252 ISS
    P granule GO:0043186 IBA
    NLRP3 inflammasome complex GO:0072559 IDA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified Ddx3x in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ddx3x
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tdrd3  
Co-fractionation Mus musculus
2 Tnik  
Affinity Capture-MS Mus musculus
3 Rps28 54127
Co-fractionation Mus musculus
4 Mycbp2 105689
Affinity Capture-MS Mus musculus
5 ABCG1  
Affinity Capture-MS Homo sapiens
6 Rps23 66475
Co-fractionation Mus musculus
7 Snd1 56463
Co-fractionation Mus musculus
8 Fmr1  
Affinity Capture-MS Mus musculus
9 Rpl6 19988
Co-fractionation Mus musculus
10 Rps19 20085
Co-fractionation Mus musculus
11 Tia1  
Co-fractionation Mus musculus
12 Tardbp 230908
Co-fractionation Mus musculus
13 Herc2  
Affinity Capture-MS Mus musculus
14 Tmem173  
Proximity Label-MS Mus musculus
15 MKRN1 23608
Affinity Capture-MS Homo sapiens
16 Rps9 76846
Co-fractionation Mus musculus
17 Rps21 66481
Co-fractionation Mus musculus
18 Zbtb7b  
Affinity Capture-MS Mus musculus
19 Eif4g1 208643
Co-fractionation Mus musculus
20 Hnrnpdl 50926
Co-fractionation Mus musculus
21 Eed  
Affinity Capture-MS Mus musculus
22 Rps27 57294
Co-fractionation Mus musculus
23 Igsf8 140559
Co-fractionation Mus musculus
24 Rptor  
Co-fractionation Mus musculus
25 EXOSC1 51013
Two-hybrid Homo sapiens
26 PDIA6 10130
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which Ddx3x is involved
PathwayEvidenceSource
Immune System IEA Reactome
Innate Immune System IEA Reactome
Neutrophil degranulation IEA Reactome





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