Gene description for Ddx3x
Gene name DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked
Gene symbol Ddx3x
Other names/aliases D1Pas1-rs2
Ddx3
Fin14
Species Mus musculus
 Database cross references - Ddx3x
ExoCarta ExoCarta_13205
Entrez Gene 13205
UniProt Q62167  
 Ddx3x identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
Pancreatic cells 19351151    
 Gene ontology annotations for Ddx3x
Molecular Function
    protein binding GO:0005515 IPI
    translation initiation factor binding GO:0031369 ISO
    CTPase activity GO:0043273 ISO
    ATPase activity GO:0016887 ISO
    ATP binding GO:0005524 IEA
    poly(A) RNA binding GO:0044822 ISO
    RNA binding GO:0003723 ISO
    nucleic acid binding GO:0003676 IEA
    ATP-dependent RNA helicase activity GO:0004004 ISO
    nucleoside-triphosphatase activity GO:0017111 ISO
    hydrolase activity GO:0016787 IEA
    RNA stem-loop binding GO:0035613 ISO
    helicase activity GO:0004386 IEA
    nucleotide binding GO:0000166 IEA
    ribosomal small subunit binding GO:0043024 ISO
    eukaryotic initiation factor 4E binding GO:0008190 ISO
    DNA binding GO:0003677 ISO
    poly(A) binding GO:0008143 ISO
    mRNA 5'-UTR binding GO:0048027 ISO
    ATP-dependent DNA helicase activity GO:0004003 ISO
    transcription factor binding GO:0008134 ISO
    GTPase activity GO:0003924 ISO
Biological Process
    negative regulation of cell growth GO:0030308 ISO
    cellular response to arsenic-containing substance GO:0071243 ISO
    translational initiation GO:0006413 IBA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    positive regulation of cell growth GO:0030307 ISO
    positive regulation of translational initiation GO:0045948 ISO
    apoptotic process GO:0006915 IEA
    chromosome segregation GO:0007059 ISO
    immune system process GO:0002376 IEA
    cellular response to osmotic stress GO:0071470 ISO
    innate immune response GO:0045087 ISO
    negative regulation of translation GO:0017148 ISO
    response to virus GO:0009615 ISO
    ribosome biogenesis GO:0042254 IEA
    transcription, DNA-templated GO:0006351 IEA
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 ISO
    regulation of transcription, DNA-templated GO:0006355 IEA
    positive regulation of gene expression GO:0010628 ISO
    DNA duplex unwinding GO:0032508 ISO
    positive regulation of apoptotic process GO:0043065 ISO
    negative regulation of protein complex assembly GO:0031333 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    positive regulation of viral genome replication GO:0045070 ISO
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 ISO
    RNA secondary structure unwinding GO:0010501 ISO
    positive regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043280 ISO
    positive regulation of translation GO:0045727 ISO
    intracellular signal transduction GO:0035556 ISO
    regulation of translation GO:0006417 IEA
    intrinsic apoptotic signaling pathway GO:0097193 ISO
    regulation of gene expression GO:0010468 IBA
    mature ribosome assembly GO:0042256 ISO
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 ISO
    stress granule assembly GO:0034063 ISO
    negative regulation of intrinsic apoptotic signaling pathway GO:2001243 ISO
Subcellular Localization
    cytoplasmic ribonucleoprotein granule GO:0036464 IBA
    eukaryotic translation initiation factor 3 complex GO:0005852 ISO
    extracellular exosome GO:0070062 ISO
    cytosolic small ribosomal subunit GO:0022627 ISO
    membrane GO:0016020 IEA
    mitochondrial outer membrane GO:0005741 IEA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISO
    cytoplasmic stress granule GO:0010494 ISO
    mitochondrion GO:0005739 IEA
 Experiment description of studies that identified Ddx3x in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Ddx3x
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ddx3x is involved
No pathways found





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