Gene description for Rps27
Gene name ribosomal protein S27
Gene symbol Rps27
Other names/aliases 3200001M24Rik
Species Mus musculus
 Database cross references - Rps27
ExoCarta ExoCarta_57294
Vesiclepedia VP_57294
Entrez Gene 57294
UniProt Q6ZWU9  
 Rps27 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Rps27
Molecular Function
    RNA binding GO:0003723 IBA
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    ribosomal small subunit assembly GO:0000028 IBA
    rRNA processing GO:0006364 ISO
    rRNA processing GO:0006364 ISS
    ribosomal small subunit biogenesis GO:0042274 ISO
    ribosomal small subunit biogenesis GO:0042274 ISS
    translation at presynapse GO:0140236 EXP
    translation at presynapse GO:0140236 IDA
    translation at presynapse GO:0140236 NAS
    translation at postsynapse GO:0140242 EXP
    translation at postsynapse GO:0140242 IDA
    translation at postsynapse GO:0140242 NAS
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleolus GO:0005730 IEA
    cytosol GO:0005829 TAS
    ribosome GO:0005840 EXP
    ribosome GO:0005840 IDA
    ribosome GO:0005840 NAS
    postsynaptic density GO:0014069 IEA
    postsynaptic density GO:0014069 ISO
    cytosolic ribosome GO:0022626 ISO
    cytosolic small ribosomal subunit GO:0022627 IBA
    cytosolic small ribosomal subunit GO:0022627 IDA
    cytosolic small ribosomal subunit GO:0022627 ISO
    small-subunit processome GO:0032040 ISO
    small-subunit processome GO:0032040 ISS
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    presynapse GO:0098793 EXP
    presynapse GO:0098793 IDA
    presynapse GO:0098793 ISO
    presynapse GO:0098793 NAS
    postsynapse GO:0098794 EXP
    postsynapse GO:0098794 IDA
    postsynapse GO:0098794 NAS
    glutamatergic synapse GO:0098978 ISO
    GABA-ergic synapse GO:0098982 ISO
 Experiment description of studies that identified Rps27 in sEVs
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Rps27
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps7 20115
Co-fractionation Mus musculus
2 Rps27a 78294
Co-fractionation Mus musculus
3 Hba 15121
Co-fractionation Mus musculus
4 Rps26 27370
Co-fractionation Mus musculus
5 Rps18 20084
Co-fractionation Mus musculus
6 Rps14 20044
Co-fractionation Mus musculus
7 Fzr1  
Affinity Capture-MS Mus musculus
8 Atxn1  
Proximity Label-MS Mus musculus
9 Rps20 67427
Co-fractionation Mus musculus
10 Eed  
Affinity Capture-MS Mus musculus
11 Kcna3  
Affinity Capture-MS Mus musculus
12 Rps21 66481
Co-fractionation Mus musculus
13 Ddx3x 13205
Co-fractionation Mus musculus
14 Atg16l1  
Affinity Capture-MS Mus musculus
15 Nanog  
Affinity Capture-MS Mus musculus
16 Fancd2  
Affinity Capture-MS Mus musculus
17 Rps3a1 20091
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Rps27 is involved
PathwayEvidenceSource
Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S IEA Reactome
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal IEA Reactome
Amplification of signal from the kinetochores IEA Reactome
Cap-dependent Translation Initiation IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
Eukaryotic Translation Initiation IEA Reactome
Formation of a pool of free 40S subunits IEA Reactome
Formation of the ternary complex, and subsequently, the 43S complex IEA Reactome
GTP hydrolysis and joining of the 60S ribosomal subunit IEA Reactome
L13a-mediated translational silencing of Ceruloplasmin expression IEA Reactome
M Phase IEA Reactome
Major pathway of rRNA processing in the nucleolus and cytosol IEA Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Mitotic Spindle Checkpoint IEA Reactome
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) IEA Reactome
Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) IEA Reactome
Nonsense-Mediated Decay (NMD) IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
Ribosomal scanning and start codon recognition IEA Reactome
rRNA processing IEA Reactome
rRNA processing in the nucleus and cytosol IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
SRP-dependent cotranslational protein targeting to membrane IEA Reactome
Translation IEA Reactome
Translation initiation complex formation IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here