Gene description for Rps27a
Gene name ribosomal protein S27A
Gene symbol Rps27a
Other names/aliases 0610006J14Rik
Uba52
Ubb
Ubc
Species Mus musculus
 Database cross references - Rps27a
ExoCarta ExoCarta_78294
Entrez Gene 78294
UniProt P62983  
 Rps27a identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Rps27a
Molecular Function
    metal ion binding GO:0046872 IEA
    poly(A) RNA binding GO:0044822 ISO
    structural constituent of ribosome GO:0003735 ISO
Biological Process
    translation GO:0006412 IEA
    biological_process GO:0008150 ND
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    cytosolic small ribosomal subunit GO:0022627 ISO
    nucleus GO:0005634 IEA
    ribosome GO:0005840 IEA
    membrane GO:0016020 ISO
    small ribosomal subunit GO:0015935 ISO
    ribonucleoprotein complex GO:0030529 IEA
    cytoplasm GO:0005737 ISO
    nucleolus GO:0005730 ISO
 Experiment description of studies that identified Rps27a in exosomes
1
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Rps27a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ACVR1 90
Affinity Capture-Luminescence Homo sapiens
2 SMURF1 57154
Affinity Capture-Luminescence Homo sapiens
3 SMAD4  
Affinity Capture-Luminescence Homo sapiens
4 SMAD2 4087
Affinity Capture-Luminescence Homo sapiens
5 BMPR1B  
Affinity Capture-Luminescence Homo sapiens
6 TGFBR1  
Affinity Capture-Luminescence Homo sapiens
View the network image/svg+xml
 Pathways in which Rps27a is involved
PathwayEvidenceSource
Activated NOTCH1 Transmits Signal to the Nucleus IEA Reactome
activated TAK1 mediates p38 MAPK activation IEA Reactome
Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon IEA Reactome
Activation of NF-kappaB in B cells IEA Reactome
Antigen processing: Ubiquitination & Proteasome degradation IEA Reactome
APC-Cdc20 mediated degradation of Nek2A IEA Reactome
APC/C:Cdc20 mediated degradation of Cyclin B IEA Reactome
APC/C:Cdc20 mediated degradation of Securin IEA Reactome
APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 IEA Reactome
Association of licensing factors with the pre-replicative complex IEA Reactome
Asymmetric localization of PCP proteins IEA Reactome
AUF1 (hnRNP D0) destabilizes mRNA IEA Reactome
Autodegradation of Cdh1 by Cdh1:APC/C IEA Reactome
Autodegradation of the E3 ubiquitin ligase COP1 IEA Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A IEA Reactome
CDK-mediated phosphorylation and removal of Cdc6 IEA Reactome
CDK-mediated phosphorylation and removal of Cdc6 IEA Reactome
CDT1 association with the CDC6:ORC:origin complex IEA Reactome
Circadian Clock IEA Reactome
Circadian Clock TAS Reactome
CLEC7A (Dectin-1) signaling IEA Reactome
Cyclin D associated events in G1 IEA Reactome
deactivation of the beta-catenin transactivating complex IEA Reactome
Dectin-1 mediated noncanonical NF-kB signaling IEA Reactome
degradation of AXIN IEA Reactome
Degradation of beta-catenin by the destruction complex IEA Reactome
degradation of DVL IEA Reactome
Degradation of GLI1 by the proteasome IEA Reactome
Degradation of GLI2 by the proteasome IEA Reactome
Downregulation of ERBB2:ERBB3 signaling IEA Reactome
Downregulation of ERBB4 signaling IEA Reactome
Downregulation of SMAD2/3:SMAD4 transcriptional activity IEA Reactome
Downregulation of TGF-beta receptor signaling IEA Reactome
Downstream TCR signaling IEA Reactome
EGFR downregulation IEA Reactome
Endosomal Sorting Complex Required For Transport (ESCRT) IEA Reactome
ER-Phagosome pathway IEA Reactome
Eukaryotic Translation Termination IEA Reactome
Fanconi Anemia pathway IEA Reactome
FCERI mediated NF-kB activation IEA Reactome
Formation of a pool of free 40S subunits IEA Reactome
Formation of the ternary complex, and subsequently, the 43S complex IEA Reactome
GLI3 is processed to GLI3R by the proteasome IEA Reactome
Glycogen synthesis IEA Reactome
GTP hydrolysis and joining of the 60S ribosomal subunit IEA Reactome
Hedgehog 'on' state IEA Reactome
Hedgehog ligand biogenesis IEA Reactome
IKK complex recruitment mediated by RIP1 IEA Reactome
Interleukin-1 signaling IEA Reactome
IRAK1 recruits IKK complex IEA Reactome
IRAK1 recruits IKK complex IEA Reactome
IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation IEA Reactome
IRAK2 mediated activation of TAK1 complex IEA Reactome
IRAK2 mediated activation of TAK1 complex IEA Reactome
IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation IEA Reactome
JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 IEA Reactome
L13a-mediated translational silencing of Ceruloplasmin expression IEA Reactome
Negative regulation of FGFR1 signaling IEA Reactome
Negative regulation of FGFR2 signaling IEA Reactome
Negative regulation of FGFR3 signaling IEA Reactome
Negative regulation of FGFR4 signaling IEA Reactome
Negative regulators of RIG-I/MDA5 signaling IEA Reactome
NF-kB is activated and signals survival IEA Reactome
NOD1/2 Signaling Pathway IEA Reactome
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) IEA Reactome
Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) IEA Reactome
NOTCH1 Intracellular Domain Regulates Transcription IEA Reactome
NOTCH2 Activation and Transmission of Signal to the Nucleus IEA Reactome
NRIF signals cell death from the nucleus IEA Reactome
Oncogene Induced Senescence IEA Reactome
Orc1 removal from chromatin IEA Reactome
Orc1 removal from chromatin IEA Reactome
Oxidative Stress Induced Senescence IEA Reactome
Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha IEA Reactome
p75NTR recruits signalling complexes IEA Reactome
Peptide chain elongation IEA Reactome
Recognition of DNA damage by PCNA-containing replication complex IEA Reactome
Regulation of activated PAK-2p34 by proteasome mediated degradation IEA Reactome
regulation of FZD by ubiquitination IEA Reactome
Regulation of innate immune responses to cytosolic DNA IEA Reactome
Regulation of necroptotic cell death IEA Reactome
Regulation of PLK1 Activity at G2/M Transition IEA Reactome
Regulation of signaling by CBL IEA Reactome
Regulation of the Fanconi anemia pathway IEA Reactome
Ribosomal scanning and start codon recognition IEA Reactome
RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways IEA Reactome
SCF(Skp2)-mediated degradation of p27/p21 IEA Reactome
SCF(Skp2)-mediated degradation of p27/p21 IEA Reactome
SCF-beta-TrCP mediated degradation of Emi1 IEA Reactome
Senescence-Associated Secretory Phenotype (SASP) IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signaling by ERBB2 IEA Reactome
SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription IEA Reactome
Spry regulation of FGF signaling IEA Reactome
Spry regulation of FGF signaling IEA Reactome
Spry regulation of FGF signaling IEA Reactome
Spry regulation of FGF signaling IEA Reactome
SRP-dependent cotranslational protein targeting to membrane IEA Reactome
Stimuli-sensing channels IEA Reactome
TAK1 activates NFkB by phosphorylation and activation of IKKs complex IEA Reactome
TCF dependent signaling in response to WNT IEA Reactome
Termination of translesion DNA synthesis IEA Reactome
TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) IEA Reactome
TRAF6 mediated induction of TAK1 complex IEA Reactome
TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling IEA Reactome
Translation initiation complex formation IEA Reactome
Translesion Synthesis by POLH IEA Reactome
Translesion synthesis by POLI IEA Reactome
Translesion synthesis by POLK IEA Reactome
Translesion synthesis by REV1 IEA Reactome
Ubiquitin Mediated Degradation of Phosphorylated Cdc25A IEA Reactome
Ubiquitin-dependent degradation of Cyclin D1 IEA Reactome





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