Gene description for Tpi1
Gene name triosephosphate isomerase 1
Gene symbol Tpi1
Other names/aliases AI255506
Tpi
Tpi-1
Species Mus musculus
 Database cross references - Tpi1
ExoCarta ExoCarta_21991
Vesiclepedia VP_21991
Entrez Gene 21991
UniProt P17751  
 Tpi1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
Mov neuroglial cells 15210972    
Neural stem cells 25242146    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Tpi1
Molecular Function
    triose-phosphate isomerase activity GO:0004807 IBA
    triose-phosphate isomerase activity GO:0004807 IDA
    triose-phosphate isomerase activity GO:0004807 IMP
    triose-phosphate isomerase activity GO:0004807 ISO
    protein binding GO:0005515 IPI
    methylglyoxal synthase activity GO:0008929 ISS
    isomerase activity GO:0016853 IDA
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    protein homodimerization activity GO:0042803 ISO
    protein homodimerization activity GO:0042803 ISS
Biological Process
    glucose metabolic process GO:0006006 IDA
    gluconeogenesis GO:0006094 IBA
    gluconeogenesis GO:0006094 IDA
    gluconeogenesis GO:0006094 IEA
    glycolytic process GO:0006096 IBA
    glycolytic process GO:0006096 IC
    multicellular organism development GO:0007275 IMP
    embryo development GO:0009790 IMP
    methylglyoxal biosynthetic process GO:0019242 ISS
    glycerol catabolic process GO:0019563 IBA
    glyceraldehyde-3-phosphate metabolic process GO:0019682 IDA
    glyceraldehyde-3-phosphate biosynthetic process GO:0046166 IBA
    glyceraldehyde-3-phosphate biosynthetic process GO:0046166 ISO
    glyceraldehyde-3-phosphate biosynthetic process GO:0046166 ISS
    canonical glycolysis GO:0061621 IGI
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified Tpi1 in sEVs
1
Experiment ID 907
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 39
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
5
Experiment ID 264
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 33
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
7
Experiment ID 188
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 908
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Tpi1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps27a 78294
Co-fractionation Mus musculus
2 Mdm2  
Affinity Capture-MS Mus musculus
3 Rpl32 19951
Co-fractionation Mus musculus
4 EEA1 8411
Affinity Capture-MS Homo sapiens
5 Kcnma1  
Affinity Capture-MS Mus musculus
6 Uchl5 56207
Co-fractionation Mus musculus
7 Atxn1  
Affinity Capture-MS Mus musculus
8 Kctd13  
Affinity Capture-MS Mus musculus
9 Tfe3  
Affinity Capture-MS Mus musculus
10 Pgam2 56012
Co-fractionation Mus musculus
11 HSD17B10 3028
Affinity Capture-MS Homo sapiens
12 Rps24 20088
Co-fractionation Mus musculus
13 Ywhaz 22631
Affinity Capture-MS Mus musculus
14 Tmem173  
Proximity Label-MS Mus musculus
15 Cox5a  
Co-fractionation Mus musculus
16 Rps20 67427
Co-fractionation Mus musculus
17 MAPT  
Reconstituted Complex Homo sapiens
18 Rps5 20103
Co-fractionation Mus musculus
19 Cdkn2a  
Two-hybrid Mus musculus
20 CHAF1A  
Affinity Capture-MS Homo sapiens
21 Actg1 11465
Co-fractionation Mus musculus
22 Rps15 20054
Co-fractionation Mus musculus
23 Invs  
Affinity Capture-MS Mus musculus
24 Fancd2  
Affinity Capture-MS Mus musculus
25 Rps26 27370
Co-fractionation Mus musculus
26 CUX1 1523
Affinity Capture-MS Homo sapiens
27 Pgk1 18655
Co-fractionation Mus musculus
28 Eed  
Affinity Capture-MS Mus musculus
29 Rpl3 27367
Co-fractionation Mus musculus
30 Atg16l1  
Affinity Capture-MS Mus musculus
31 Rps17 20068
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Tpi1 is involved
PathwayEvidenceSource
Gluconeogenesis IEA Reactome
Glucose metabolism IEA Reactome
Glycolysis IEA Reactome
Metabolism IEA Reactome
Metabolism of carbohydrates IEA Reactome





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