Gene description for Rps5
Gene name ribosomal protein S5
Gene symbol Rps5
Other names/aliases AA617411
Species Mus musculus
 Database cross references - Rps5
ExoCarta ExoCarta_20103
Vesiclepedia VP_20103
Entrez Gene 20103
UniProt P97461  
 Rps5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rps5
Molecular Function
    mRNA binding GO:0003729 IBA
    mRNA binding GO:0003729 IEA
    mRNA binding GO:0003729 ISO
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
    rRNA binding GO:0019843 IBA
Biological Process
    ribosomal small subunit assembly GO:0000028 IBA
    cytoplasmic translation GO:0002181 NAS
    translation GO:0006412 IBA
    translation GO:0006412 ISO
    regulation of translational fidelity GO:0006450 IEA
    regulation of translational fidelity GO:0006450 ISO
    ribosomal small subunit biogenesis GO:0042274 ISO
    ribosomal small subunit biogenesis GO:0042274 ISS
    translation at presynapse GO:0140236 NAS
    translation at postsynapse GO:0140242 NAS
Subcellular Localization
    nucleolus GO:0005730 IEA
    cytoplasm GO:0005737 NAS
    cytosol GO:0005829 TAS
    ribosome GO:0005840 IBA
    ribosome GO:0005840 NAS
    cytosolic ribosome GO:0022626 ISO
    cytosolic small ribosomal subunit GO:0022627 IBA
    cytosolic small ribosomal subunit GO:0022627 IDA
    cytosolic small ribosomal subunit GO:0022627 ISO
    cytosolic small ribosomal subunit GO:0022627 ISS
    cytosolic small ribosomal subunit GO:0022627 NAS
    small-subunit processome GO:0032040 ISO
    small-subunit processome GO:0032040 ISS
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    presynapse GO:0098793 NAS
    postsynapse GO:0098794 NAS
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Rps5 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rps5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps27a 78294
Co-fractionation Mus musculus
2 Rps3 27050
Co-fractionation Mus musculus
3 Rpl11 67025
Co-fractionation Mus musculus
4 Ola1 67059
Co-fractionation Mus musculus
5 Rps18 20084
Co-fractionation Mus musculus
6 Fancd2  
Affinity Capture-MS Mus musculus
7 Rps10 67097
Co-fractionation Mus musculus
8 Olfm1  
Affinity Capture-MS Mus musculus
9 Eif5a 276770
Co-fractionation Mus musculus
10 Fus  
Co-fractionation Mus musculus
11 Rplp2 67186
Co-fractionation Mus musculus
12 Eif3b 27979
Co-fractionation Mus musculus
13 Rps4x 20102
Co-fractionation Mus musculus
14 Rps2 16898
Co-fractionation Mus musculus
15 Rpl32 19951
Co-fractionation Mus musculus
16 Rps8 20116
Co-fractionation Mus musculus
17 Pkm 18746
Co-fractionation Mus musculus
18 Rps23 66475
Co-fractionation Mus musculus
19 Rpl7a 27176
Co-fractionation Mus musculus
20 Rpl31 114641
Co-fractionation Mus musculus
21 Rpl18 19899
Co-fractionation Mus musculus
22 Rpl3 27367
Co-fractionation Mus musculus
23 Dync1i2  
Co-fractionation Mus musculus
24 Rpl6 19988
Co-fractionation Mus musculus
25 Kctd13  
Affinity Capture-MS Mus musculus
26 Rps19 20085
Co-fractionation Mus musculus
27 Nob1  
Co-fractionation Mus musculus
28 Tfe3  
Affinity Capture-MS Mus musculus
29 Rpl9 20005
Co-fractionation Mus musculus
30 Rpl24 68193
Co-fractionation Mus musculus
31 Rpl35 66489
Co-fractionation Mus musculus
32 Tpt1 22070
Co-fractionation Mus musculus
33 Rps11 27207
Co-fractionation Mus musculus
34 Rpl18a 76808
Co-fractionation Mus musculus
35 Eef1a2 13628
Co-fractionation Mus musculus
36 Rpl13 270106
Co-fractionation Mus musculus
37 Rps7 20115
Co-fractionation Mus musculus
38 Rpl38  
Co-fractionation Mus musculus
39 Rpl22 19934
Co-fractionation Mus musculus
40 Rps16 20055
Co-fractionation Mus musculus
41 Tmem173  
Proximity Label-MS Mus musculus
42 Fzr1  
Affinity Capture-MS Mus musculus
43 Rpl15 66480
Co-fractionation Mus musculus
44 Eef2 13629
Co-fractionation Mus musculus
45 Rps24 20088
Co-fractionation Mus musculus
46 Rps13 68052
Co-fractionation Mus musculus
47 Rpl12 269261
Co-fractionation Mus musculus
48 Eef1a1 13627
Co-fractionation Mus musculus
49 Rps21 66481
Co-fractionation Mus musculus
50 Rpl7 19989
Co-fractionation Mus musculus
51 Rps15a 267019
Co-fractionation Mus musculus
52 Rps28 54127
Co-fractionation Mus musculus
53 Gnb2l1 14694
Co-fractionation Mus musculus
54 Rps15 20054
Co-fractionation Mus musculus
55 Pgk2 18663
Co-fractionation Mus musculus
56 Rps3a1 20091
Co-fractionation Mus musculus
57 Npm1 18148
Affinity Capture-MS Mus musculus
58 Rpl26 19941
Co-fractionation Mus musculus
59 Rps20 67427
Co-fractionation Mus musculus
60 Rplp0 11837
Co-fractionation Mus musculus
61 Rpl23a 268449
Co-fractionation Mus musculus
62 Rps14 20044
Co-fractionation Mus musculus
63 Pgk1 18655
Co-fractionation Mus musculus
64 Rps6 20104
Co-fractionation Mus musculus
65 Vdac3 22335
Co-fractionation Mus musculus
66 Eed  
Affinity Capture-MS Mus musculus
67 Rpsa 16785
Co-fractionation Mus musculus
68 Eef1b2 55949
Co-fractionation Mus musculus
69 Tpi1 21991
Co-fractionation Mus musculus
70 Riok2  
Co-fractionation Mus musculus
71 Rpa3  
Co-fractionation Mus musculus
72 Rpl5 100503670
Co-fractionation Mus musculus
73 Dnajc2  
Co-fractionation Mus musculus
74 Rps17 20068
Co-fractionation Mus musculus
75 Rpl8 26961
Co-fractionation Mus musculus
76 Rps12 20042
Co-fractionation Mus musculus
77 Eif2a  
Co-fractionation Mus musculus
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