Gene description for Eif3b
Gene name eukaryotic translation initiation factor 3, subunit B
Gene symbol Eif3b
Other names/aliases AL033316
AL033334
AL033369
AW208965
D5Wsu45e
EIF3-ETA
EIF3-P110
EIF3-P116
Eif3s9
PRT1
Species Mus musculus
 Database cross references - Eif3b
ExoCarta ExoCarta_27979
Vesiclepedia VP_27979
Entrez Gene 27979
UniProt Q8JZQ9  
 Eif3b identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eif3b
Molecular Function
    RNA binding GO:0003723 IEA
    RNA binding GO:0003723 ISO
    translation initiation factor activity GO:0003743 IBA
    translation initiation factor activity GO:0003743 IDA
    translation initiation factor activity GO:0003743 ISO
    translation initiation factor activity GO:0003743 ISO
    protein binding GO:0005515 IPI
    translation initiation factor binding GO:0031369 IEA
Biological Process
    formation of cytoplasmic translation initiation complex GO:0001732 IEA
    translational initiation GO:0006413 IBA
    translational initiation GO:0006413 IDA
    translational initiation GO:0006413 ISO
    regulation of translational initiation GO:0006446 IEA
    regulation of translational initiation GO:0006446 ISO
    IRES-dependent viral translational initiation GO:0075522 IEA
    IRES-dependent viral translational initiation GO:0075522 ISO
    viral translational termination-reinitiation GO:0075525 IEA
    viral translational termination-reinitiation GO:0075525 ISO
Subcellular Localization
    eukaryotic translation initiation factor 3 complex GO:0005852 IBA
    eukaryotic translation initiation factor 3 complex GO:0005852 IDA
    eukaryotic translation initiation factor 3 complex GO:0005852 ISO
    cytoplasmic stress granule GO:0010494 ISO
    cytoplasmic stress granule GO:0010494 ISS
    eukaryotic 43S preinitiation complex GO:0016282 IEA
    eukaryotic 48S preinitiation complex GO:0033290 IEA
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    eukaryotic translation initiation factor 3 complex, eIF3m GO:0071541 IDA
 Experiment description of studies that identified Eif3b in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eif3b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mettl21e  
Affinity Capture-MS Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Fzr1  
Affinity Capture-MS Mus musculus
4 Eed  
Affinity Capture-MS Mus musculus
5 Upf1 19704
Co-fractionation Mus musculus
6 Rps5 20103
Co-fractionation Mus musculus
7 Riok2  
Co-fractionation Mus musculus
8 Atg16l1  
Affinity Capture-MS Mus musculus
9 Eif1 20918
Co-fractionation Mus musculus
10 Chaf1a  
Affinity Capture-MS Mus musculus
11 Fancd2  
Affinity Capture-MS Mus musculus
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