Experiment description of studies that identified Eef1a2 in exosomes

1 Experiment ID 210 MISEV standards ✔ EM Biophysical techniques ✔ CD81|FLOT1 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23260141     Organism Mus musculus Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration. Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL. Journal name Cell Publication year 2012 Sample Fibroblasts Sample name Normal-Fibroblasts (L cells) Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 15 MISEV standards ✔ EM Biophysical techniques ✔ CD63 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 17486113     Organism Mus musculus Homo sapiens Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO Journal name NCB Publication year 2007 Sample Mast cells Sample name MC9 Bone marrow-derived mast cells HMC-1 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.11-1.21 g/mL Molecules identified in the study Protein mRNA miRNA Methods used in the study Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array

3 Experiment ID 33 MISEV standards ✔ EM|IEM Biophysical techniques ✔ Alix|TSG101 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 21136642     Organism Mus musculus Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons? Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J Journal name PROTEOMICS_CL Publication year 2007 Sample Oligodendrocytes Sample name Oligodendrocytes Oli-neu Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.10-1.14 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [QTOF] Western blotting Immunoelectron Microscopy

Protein-protein interactions for Eef1a2

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Phb2 12034 Affinity Capture-Western Mus musculus 2 Rpl19 19921 Co-fractionation Mus musculus 3 Uvrag   Affinity Capture-MS Mus musculus 4 Lmb1   Affinity Capture-Western Mus musculus 5 Atxn1   Proximity Label-MS Mus musculus 6 Cyfip1 20430 Affinity Capture-MS Mus musculus 7 Kctd13   Affinity Capture-MS Mus musculus 8 Thrb   Affinity Capture-MS Mus musculus 9 MAPT   Reconstituted Complex Homo sapiens 10 Srsf3 20383 Affinity Capture-Western Mus musculus 11 Ubxn6   Co-fractionation Mus musculus 12 Htt   Affinity Capture-MS Mus musculus 13 Usp48   Affinity Capture-MS Mus musculus 14 Plcb1   Affinity Capture-Western Rattus norvegicus 15 Eef1a2 13628 Cross-Linking-MS (XL-MS) Mus musculus Cross-Linking-MS (XL-MS) Mus musculus 16 Ywhae 22627 Affinity Capture-MS Mus musculus 17 Rps5 20103 Co-fractionation Mus musculus 18 Lipc   Affinity Capture-MS Mus musculus View the network

Pathways in which Eef1a2 is involved

No pathways found