ExoCarta: Gene summary

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Gene description for Rps15a
Gene name	ribosomal protein S15A
Gene symbol	Rps15a
Other names/aliases	A630031B11Rik
Species	Mus musculus
Database cross references - Rps15a
ExoCarta	ExoCarta_267019
Entrez Gene	267019
UniProt	P62245
Rps15a identified in exosomes derived from the following tissue/cell type
Mast cells	17486113

Gene ontology annotations for Rps15a

Molecular Function
	poly(A) RNA binding	GO:0044822	ISO
	structural constituent of ribosome	GO:0003735	ISO
Biological Process
	response to virus	GO:0009615	ISO
	positive regulation of cell cycle	GO:0045787	ISO
	positive regulation of cell proliferation	GO:0008284	ISO
Subcellular Localization
	cytosolic small ribosomal subunit	GO:0022627	ISO
	cytoplasm	GO:0005737	ISO
	extracellular exosome	GO:0070062	ISO
	mitochondrion	GO:0005739	IDA
	membrane	GO:0016020	ISO

Experiment description of studies that identified Rps15a in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✘	EV Cytosolic markers
✔ CD63	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

17486113

Organism

Mus musculus
Homo sapiens

Experiment description

Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.

Authors

Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO

Journal name

NCB

Publication year

2007

Sample

Mast cells

Sample name

MC9
Bone marrow-derived mast cells
HMC-1

Isolation/purification methods

Filtration
Ultracentrifugation
Sucrose density gradient

Flotation density

1.11-1.21 g/mL

Molecules identified in the study

Protein
mRNA
miRNA

Methods used in the study

Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array