Gene description for Rpl26
Gene name ribosomal protein L26
Gene symbol Rpl26
Other names/aliases SIG-20
Species Mus musculus
 Database cross references - Rpl26
ExoCarta ExoCarta_19941
Vesiclepedia VP_19941
Entrez Gene 19941
UniProt P61255  
 Rpl26 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rpl26
Molecular Function
    RNA binding GO:0003723 IBA
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
    protein binding GO:0005515 IPI
    mRNA 5'-UTR binding GO:0048027 IEA
    mRNA 5'-UTR binding GO:0048027 ISO
Biological Process
    cytoplasmic translation GO:0002181 IBA
    cytoplasmic translation GO:0002181 NAS
    rRNA processing GO:0006364 IEA
    rRNA processing GO:0006364 ISO
    DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest GO:0006977 IDA
    DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest GO:0006977 ISO
    cellular response to UV GO:0034644 ISO
    ribosomal large subunit biogenesis GO:0042273 IBA
    ribosomal large subunit biogenesis GO:0042273 IEA
    ribosomal large subunit biogenesis GO:0042273 ISO
    positive regulation of translation GO:0045727 IEA
    positive regulation of translation GO:0045727 ISO
    cellular response to ionizing radiation GO:0071479 IMP
    cellular response to gamma radiation GO:0071480 IEA
    cellular response to gamma radiation GO:0071480 ISO
    translation at presynapse GO:0140236 NAS
    translation at postsynapse GO:0140242 NAS
    positive regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator GO:1902164 IEA
    positive regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator GO:1902164 ISO
    positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:1902167 IMP
    positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:1902167 ISO
    regulation of translation involved in cellular response to UV GO:1904803 IEA
    regulation of translation involved in cellular response to UV GO:1904803 ISO
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 TAS
    ribosome GO:0005840 NAS
    cytosolic large ribosomal subunit GO:0022625 IBA
    cytosolic large ribosomal subunit GO:0022625 IDA
    cytosolic large ribosomal subunit GO:0022625 IPI
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic ribosome GO:0022626 ISO
    terminal bouton GO:0043195 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    presynapse GO:0098793 NAS
    postsynapse GO:0098794 NAS
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Rpl26 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rpl26
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Co-fractionation Mus musculus
2 Rpl28 19943
Co-fractionation Mus musculus
3 Rplp0 11837
Co-fractionation Mus musculus
4 Atxn2 20239
Co-fractionation Mus musculus
5 Rps18 20084
Co-fractionation Mus musculus
6 Rpl27 19942
Co-fractionation Mus musculus
7 Ybx1 22608
Co-fractionation Mus musculus
8 Fmr1  
Co-fractionation Mus musculus
9 Rpl6 19988
Co-fractionation Mus musculus
10 Rps6 20104
Co-fractionation Mus musculus
11 Ncl 17975
Co-fractionation Mus musculus
12 Actb 11461
Co-fractionation Mus musculus
13 Rpl7 19989
Co-fractionation Mus musculus
14 Eef1a1 13627
Co-fractionation Mus musculus
15 Rps9 76846
Co-fractionation Mus musculus
16 Rpl36a 19982
Co-fractionation Mus musculus
17 Rpsa 16785
Co-fractionation Mus musculus
18 Rpl3 27367
Co-fractionation Mus musculus
19 Rpl11 67025
Co-fractionation Mus musculus
20 Eed  
Affinity Capture-MS Mus musculus
21 Elf5  
Affinity Capture-MS Mus musculus
22 Rpl31 114641
Co-fractionation Mus musculus
23 Rps10 67097
Co-fractionation Mus musculus
24 Eif5a 276770
Co-fractionation Mus musculus
25 Rplp2 67186
Co-fractionation Mus musculus
26 Rps4x 20102
Co-fractionation Mus musculus
27 Trove2 20822
Co-fractionation Mus musculus
28 Rps23 66475
Co-fractionation Mus musculus
29 Mapt  
Affinity Capture-MS Mus musculus
30 Tfe3  
Affinity Capture-MS Mus musculus
31 Rpl24 68193
Co-fractionation Mus musculus
32 Rps7 20115
Co-fractionation Mus musculus
33 Rpl17 319195
Co-fractionation Mus musculus
34 Pabpc1 18458
Co-fractionation Mus musculus
35 Rps5 20103
Co-fractionation Mus musculus
36 Actn4 60595
Co-fractionation Mus musculus
37 Rps15a 267019
Co-fractionation Mus musculus
38 Rps20 67427
Co-fractionation Mus musculus
39 Dhx9 13211
Co-fractionation Mus musculus
40 Rpl30 19946
Co-fractionation Mus musculus
41 Rpl39 67248
Co-fractionation Mus musculus
42 Rps16 20055
Co-fractionation Mus musculus
43 Rps17 20068
Co-fractionation Mus musculus
44 Raver1  
Co-fractionation Mus musculus
45 Rpl13a 22121
Co-fractionation Mus musculus
46 Rpl14 67115
Co-fractionation Mus musculus
47 Rps2 16898
Co-fractionation Mus musculus
48 Gapdh 14433
Co-fractionation Mus musculus
49 Rps8 20116
Co-fractionation Mus musculus
50 Rpl7a 27176
Co-fractionation Mus musculus
51 Rpl32 19951
Co-fractionation Mus musculus
52 Rps28 54127
Co-fractionation Mus musculus
53 Rps19 20085
Co-fractionation Mus musculus
54 Tpt1 22070
Co-fractionation Mus musculus
55 Rps11 27207
Co-fractionation Mus musculus
56 Rpl18a 76808
Co-fractionation Mus musculus
57 Rpl22 19934
Co-fractionation Mus musculus
58 Rps24 20088
Co-fractionation Mus musculus
59 Rpl21 19933
Co-fractionation Mus musculus
60 Rpl38  
Co-fractionation Mus musculus
61 Syncrip 56403
Co-fractionation Mus musculus
62 Rps3a1 20091
Co-fractionation Mus musculus
63 Rps21 66481
Co-fractionation Mus musculus
64 Rps14 20044
Co-fractionation Mus musculus
65 Rpl35 66489
Co-fractionation Mus musculus
66 Rpl5 100503670
Co-fractionation Mus musculus
67 Fancd2  
Affinity Capture-MS Mus musculus
68 Rps27a 78294
Co-fractionation Mus musculus
69 Ncbp1 433702
Co-fractionation Mus musculus
70 Rps25 75617
Co-fractionation Mus musculus
71 Eef1e1  
Co-fractionation Mus musculus
72 Rpl10a 19896
Co-fractionation Mus musculus
73 Kctd13  
Affinity Capture-MS Mus musculus
74 Fzr1  
Affinity Capture-MS Mus musculus
75 Rpl12 269261
Co-fractionation Mus musculus
76 Rpl18 19899
Co-fractionation Mus musculus
77 Gnb2l1 14694
Co-fractionation Mus musculus
78 Rps15 20054
Co-fractionation Mus musculus
79 Rps13 68052
Co-fractionation Mus musculus
80 Hnrnpa2b1 53379
Co-fractionation Mus musculus
81 Rpl23 65019
Co-fractionation Mus musculus
82 Rpl23a 268449
Co-fractionation Mus musculus
83 Rpl15 66480
Co-fractionation Mus musculus
84 Eef1b2 55949
Co-fractionation Mus musculus
85 Igsf8 140559
Co-fractionation Mus musculus
86 Rpl4 67891
Co-fractionation Mus musculus
87 Rpl13 270106
Co-fractionation Mus musculus
88 Rpl8 26961
Co-fractionation Mus musculus
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