Gene description for Actn4
Gene name actinin alpha 4
Gene symbol Actn4
Other names/aliases C77391
Species Mus musculus
 Database cross references - Actn4
ExoCarta ExoCarta_60595
Vesiclepedia VP_60595
Entrez Gene 60595
UniProt P57780  
 Actn4 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
Mast cells 17486113    
Mov neuroglial cells 15210972    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Actn4
Molecular Function
    poly(A) RNA binding GO:0044822 ISO
    protein homodimerization activity GO:0042803 TAS
    ligand-dependent nuclear receptor transcription coactivator activity GO:0030374 ISO
    nuclear hormone receptor binding GO:0035257 ISO
    protein complex binding GO:0032403 ISO
    actin filament binding GO:0051015 ISO
    calcium ion binding GO:0005509 IEA
    actin binding GO:0003779 IEA
    protein binding GO:0005515 IPI
    retinoic acid receptor binding GO:0042974 ISO
    metal ion binding GO:0046872 IEA
    nucleoside binding GO:0001882 ISO
    protein N-terminus binding GO:0047485 ISO
Biological Process
    negative regulation of cellular component movement GO:0051271 IDA
    positive regulation of cellular component movement GO:0051272 ISO
    peroxisome proliferator activated receptor signaling pathway GO:0035357 ISO
    actin filament bundle assembly GO:0051017 IDA
    transport GO:0006810 IEA
    positive regulation of pinocytosis GO:0048549 IMP
    bicellular tight junction assembly GO:0070830 IMP
    regulation of nucleic acid-templated transcription GO:1903506 ISO
    regulation of apoptotic process GO:0042981 ISO
    protein localization to bicellular tight junction GO:1902396 IMP
    retinoic acid receptor signaling pathway GO:0048384 ISO
    protein transport GO:0015031 IEA
Subcellular Localization
    focal adhesion GO:0005925 ISO
    ribonucleoprotein complex GO:0030529 ISO
    pseudopodium GO:0031143 IDA
    neuron projection GO:0043005 ISO
    extracellular exosome GO:0070062 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISO
    stress fiber GO:0001725 ISO
    protein complex GO:0043234 ISO
    Z disc GO:0030018 IDA
    cell-cell junction GO:0005911 IDA
    cortical cytoskeleton GO:0030863 IDA
    cell junction GO:0030054 IEA
    extracellular space GO:0005615 ISO
 Experiment description of studies that identified Actn4 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 210
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD81|FLOT1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 15
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
5
Experiment ID 39
MISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|HSC70|FLOT1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
6
Experiment ID 188
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Actn4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 1300006N24Rik  
Two-hybrid Mus musculus
View the network image/svg+xml
 Pathways in which Actn4 is involved
PathwayEvidenceSource
Nephrin interactions IEA Reactome
Platelet degranulation IEA Reactome





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