Gene description for Rpl27
Gene name ribosomal protein L27
Gene symbol Rpl27
Other names/aliases -
Species Mus musculus
 Database cross references - Rpl27
ExoCarta ExoCarta_19942
Entrez Gene 19942
UniProt P61358  
 Rpl27 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Rpl27
Molecular Function
    poly(A) RNA binding GO:0044822 ISO
Subcellular Localization
    ribonucleoprotein complex GO:0030529 ISO
    membrane GO:0016020 ISO
    extracellular exosome GO:0070062 ISO
    focal adhesion GO:0005925 ISO
    cytosolic large ribosomal subunit GO:0022625 ISO
    nucleus GO:0005634 ISO
 Experiment description of studies that identified Rpl27 in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Rpl27
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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