Gene description for Eif5a
Gene name eukaryotic translation initiation factor 5A
Gene symbol Eif5a
Other names/aliases AA410058
D19Wsu54e
Eif4d
Eif5a1
eIF-4D
eIF-5A
eIF-5A-1
eIF-5A1
Species Mus musculus
 Database cross references - Eif5a
ExoCarta ExoCarta_276770
Vesiclepedia VP_276770
Entrez Gene 276770
UniProt P63242  
 Eif5a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eif5a
Molecular Function
    RNA binding GO:0003723 IEA
    RNA binding GO:0003723 ISO
    translation elongation factor activity GO:0003746 IBA
    translation elongation factor activity GO:0003746 IEA
    U6 snRNA binding GO:0017070 ISO
    ribosome binding GO:0043022 IEA
Biological Process
    translational elongation GO:0006414 IBA
    translational elongation GO:0006414 ISS
    positive regulation of cytosolic calcium ion concentration GO:0007204 ISO
    positive regulation of cardiac muscle cell apoptotic process GO:0010666 ISO
    positive regulation of apoptotic process GO:0043065 IDA
    negative regulation of apoptotic process GO:0043066 ISO
    positive regulation of translational elongation GO:0045901 IEA
    positive regulation of translational termination GO:0045905 IEA
    positive regulation of muscle cell differentiation GO:0051149 ISO
    positive regulation of reactive oxygen species metabolic process GO:2000379 ISO
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    annulate lamellae GO:0005642 ISO
    nuclear pore GO:0005643 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    endoplasmic reticulum membrane GO:0005789 IEA
    dendrite GO:0030425 ISO
    neuronal cell body GO:0043025 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    synapse GO:0045202 ISO
 Experiment description of studies that identified Eif5a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eif5a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps25 75617
Co-fractionation Mus musculus
2 Rpl13a 22121
Co-fractionation Mus musculus
3 Rplp2 67186
Co-fractionation Mus musculus
4 Rps4x 20102
Co-fractionation Mus musculus
5 Atxn1  
Affinity Capture-MS Mus musculus
6 Rps19 20085
Co-fractionation Mus musculus
7 Tfe3  
Affinity Capture-MS Mus musculus
8 Rpl9 20005
Co-fractionation Mus musculus
9 Tpt1 22070
Co-fractionation Mus musculus
10 Tmem173  
Proximity Label-MS Mus musculus
11 EIF4ENIF1 56478
Two-hybrid Homo sapiens
12 Rps13 68052
Co-fractionation Mus musculus
13 Rpl7 19989
Co-fractionation Mus musculus
14 Xrn2  
Co-fractionation Mus musculus
15 Rps15 20054
Co-fractionation Mus musculus
16 Rps5 20103
Co-fractionation Mus musculus
17 Rps3a1 20091
Co-fractionation Mus musculus
18 HACE1 57531
Affinity Capture-MS Homo sapiens
19 Rpl26 19941
Co-fractionation Mus musculus
20 Rps14 20044
Co-fractionation Mus musculus
21 Eed  
Affinity Capture-MS Mus musculus
22 Rpl15 66480
Co-fractionation Mus musculus
23 Rpl32 19951
Co-fractionation Mus musculus
24 Atg16l1  
Affinity Capture-MS Mus musculus
25 Rpl4 67891
Co-fractionation Mus musculus
26 Rps17 20068
Co-fractionation Mus musculus
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