Gene description for Eif5a
Gene name eukaryotic translation initiation factor 5A
Gene symbol Eif5a
Other names/aliases AA410058
D19Wsu54e
Eif4d
Eif5a1
eIF-4D
eIF-5A
eIF-5A-1
eIF-5A1
Species Mus musculus
 Database cross references - Eif5a
ExoCarta ExoCarta_276770
Entrez Gene 276770
UniProt P63242  
 Eif5a identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Eif5a
Molecular Function
    protein N-terminus binding GO:0047485 ISO
    RNA binding GO:0003723 ISO
    U6 snRNA binding GO:0017070 ISO
    poly(A) RNA binding GO:0044822 ISO
    translation elongation factor activity GO:0003746 IEA
    ribosome binding GO:0043022 IEA
Biological Process
    apoptotic process GO:0006915 ISO
    positive regulation of translational termination GO:0045905 IEA
    positive regulation of translational elongation GO:0045901 IEA
    mRNA export from nucleus GO:0006406 ISO
    transport GO:0006810 IEA
    protein transport GO:0015031 IEA
    mRNA transport GO:0051028 IEA
    translational frameshifting GO:0006452 IEA
    protein export from nucleus GO:0006611 ISO
    translation GO:0006412 IEA
    positive regulation of cell proliferation GO:0008284 ISO
    positive regulation of apoptotic process GO:0043065 IDA
    positive regulation of muscle cell differentiation GO:0051149 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    translational elongation GO:0006414 IEA
Subcellular Localization
    nuclear pore GO:0005643 ISO
    extracellular exosome GO:0070062 ISO
    endoplasmic reticulum GO:0005783 IEA
    neuronal cell body GO:0043025 ISO
    annulate lamellae GO:0005642 ISO
    nucleus GO:0005634 ISO
    dendrite GO:0030425 ISO
    cytoplasm GO:0005737 ISO
    membrane GO:0016020 ISO
 Experiment description of studies that identified Eif5a in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Eif5a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Eif5a is involved
No pathways found





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