Gene description for Dhx9
Gene name DEAH (Asp-Glu-Ala-His) box polypeptide 9
Gene symbol Dhx9
Other names/aliases AI326842
Ddx9
HEL-5
NDHII
RHA
mHEL-5
Species Mus musculus
 Database cross references - Dhx9
ExoCarta ExoCarta_13211
Vesiclepedia VP_13211
Entrez Gene 13211
UniProt O70133  
 Dhx9 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dhx9
Molecular Function
    RNA binding GO:0003723 IEA
    helicase activity GO:0004386 IEA
    ATP-dependent RNA helicase activity GO:0004004 IBA
    poly(A) RNA binding GO:0044822 ISO
    RNA polymerase II transcription factor binding GO:0001085 ISO
    nucleic acid binding GO:0003676 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP-dependent helicase activity GO:0008026 IEA
    nucleotide binding GO:0000166 IEA
    hydrolase activity GO:0016787 IEA
    DNA binding GO:0003677 IEA
Biological Process
    circadian rhythm GO:0007623 IMP
    cellular response to heat GO:0034605 IDA
    rhythmic process GO:0048511 IEA
    RNA processing GO:0006396 IBA
    osteoblast differentiation GO:0001649 ISO
    CRD-mediated mRNA stabilization GO:0070934 ISO
Subcellular Localization
    CRD-mediated mRNA stability complex GO:0070937 ISO
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    nucleolus GO:0005730 ISO
    centrosome GO:0005813 ISO
    ribonucleoprotein complex GO:0030529 ISO
    nucleus GO:0005634 ISO
    membrane GO:0016020 ISO
 Experiment description of studies that identified Dhx9 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dhx9
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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