ExoCarta: Gene summary

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Gene description for Rpl21
Gene name	ribosomal protein L21
Gene symbol	Rpl21
Other names/aliases	8430440E03Rik L21
Species	Mus musculus
Database cross references - Rpl21
ExoCarta	ExoCarta_19933
Vesiclepedia	VP_19933
Entrez Gene	19933
Rpl21 identified in sEVs derived from the following tissue/cell type
Colon cancer cells	37309723
Mast cells	17486113
T-cell lymphoma cells	37309723

Gene ontology annotations for Rpl21

Molecular Function
	structural constituent of ribosome	GO:0003735	IBA
	structural constituent of ribosome	GO:0003735	IDA
	structural constituent of ribosome	GO:0003735	ISO
	protein binding	GO:0005515	IPI
Biological Process
	cytoplasmic translation	GO:0002181	NAS
Subcellular Localization
	cytoplasm	GO:0005737	IDA
	endoplasmic reticulum	GO:0005783	IEA
	cytosol	GO:0005829	TAS
	ribosome	GO:0005840	ISO
	ribosome	GO:0005840	NAS
	cytosolic large ribosomal subunit	GO:0022625	IBA
	cytosolic large ribosomal subunit	GO:0022625	IDA
	cytosolic large ribosomal subunit	GO:0022625	IPI
	cytosolic large ribosomal subunit	GO:0022625	ISO
	cytosolic ribosome	GO:0022626	ISO
	synapse	GO:0045202	EXP
	synapse	GO:0045202	IDA
	postsynapse	GO:0098794	NAS

Experiment description of studies that identified Rpl21 in sEVs

Experiment ID

907

MISEV standards
✔ EM	Biophysical techniques
✔ Tsg101\|Sdcbp\|Cd151\|Gapdh\|Lamp2\|Cd81\|Cd82\|Cd9\|Cd63\|Cd80\|Flot2\|Tfrc\|Rab35\|Rab5a	Enriched markers
✔ HSPA5	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

37309723

Organism

Mus musculus

Experiment description

Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells

Authors

"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."

Journal name

Proteomics

Publication year

2023

Sample

Colon cancer cells

Sample name

C26

Isolation/purification methods

Differential centrifugation
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

MISEV standards
✔ EM	Biophysical techniques
✔ CD63	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

17486113

Organism

Mus musculus
Homo sapiens

Experiment description

Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.

Authors

"Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"

Journal name

NCB

Publication year

2007

Sample

Mast cells

Sample name

MC9
Bone marrow-derived mast cells
HMC-1

Isolation/purification methods

Filtration
Ultracentrifugation
Sucrose density gradient

Flotation density

1.11-1.21 g/mL

Molecules identified in the study

Protein
mRNA
miRNA

Methods used in the study

Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array

Experiment ID

MISEV standards
✔ EM	Biophysical techniques
✔ CD63	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

mrna

Identification method

Microarray

PubMed ID

17486113

Organism

Mus musculus
Homo sapiens

Experiment description

Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.

Authors

"Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"

Journal name

NCB

Publication year

2007

Sample

Mast cells

Sample name

MC9
Bone marrow-derived mast cells
HMC-1

Isolation/purification methods

Filtration
Ultracentrifugation
Sucrose density gradient

Flotation density

1.11-1.21 g/mL

Molecules identified in the study

Protein
mRNA
miRNA

Methods used in the study

Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array

Experiment ID

908

MISEV standards
✔ EM	Biophysical techniques
✔ Tsg101\|Sdcbp\|Cd151\|Gapdh\|Lamp2\|Cd81\|Cd82\|Cd9\|Cd63\|Cd80\|Flot2\|Tfrc\|Rab35\|Rab5a	Enriched markers
✔ HSPA5	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

37309723

Organism

Mus musculus

Experiment description

Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells

Authors

"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."

Journal name

Proteomics

Publication year

2023

Sample

T-cell lymphoma cells

Sample name

EL4

Isolation/purification methods

Differential centrifugation
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Protein-protein interactions for Rpl21

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

Rpl28

19943

Co-fractionation

Mus musculus

Rpl35

66489

Co-fractionation

Mus musculus

Rpl31

114641

Co-fractionation

Mus musculus

Rpl11

67025

Co-fractionation

Mus musculus

Rplp2

67186

Co-fractionation

Mus musculus

Rpl14

67115

Co-fractionation

Mus musculus

Rpl10a

19896

Co-fractionation

Mus musculus

Rpl6

19988

Co-fractionation

Mus musculus

Kctd13

Affinity Capture-MS

Mus musculus

Rpl24

68193

Co-fractionation

Mus musculus

Rps11

27207

Co-fractionation

Mus musculus

Rpl17

319195

Co-fractionation

Mus musculus

Fzr1

Affinity Capture-MS

Mus musculus

Rpl7

19989

Co-fractionation

Mus musculus

Rpl18

19899

Co-fractionation

Mus musculus

Rpl38

Co-fractionation

Mus musculus

Rpl36a

19982

Co-fractionation

Mus musculus

Rpl26

19941

Co-fractionation

Mus musculus

Rpl13

270106

Co-fractionation

Mus musculus

Rpl23

65019

Co-fractionation

Mus musculus

Rpl30

19946

Co-fractionation

Mus musculus

Eed

Affinity Capture-MS

Mus musculus

Rpl15

66480

Co-fractionation

Mus musculus

Rpl5

100503670

Co-fractionation

Mus musculus

Fancd2

Affinity Capture-MS

Mus musculus

Rpl8

26961

Co-fractionation

Mus musculus

View the network

Pathways in which Rpl21 is involved

Pathway	Evidence	Source
Cap-dependent Translation Initiation	IEA	Reactome
Eukaryotic Translation Initiation	IEA	Reactome
Formation of a pool of free 40S subunits	IEA	Reactome
GTP hydrolysis and joining of the 60S ribosomal subunit	IEA	Reactome
L13a-mediated translational silencing of Ceruloplasmin expression	IEA	Reactome
Major pathway of rRNA processing in the nucleolus and cytosol	IEA	Reactome
Metabolism of proteins	IEA	Reactome
Metabolism of RNA	IEA	Reactome
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)	IEA	Reactome
Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)	IEA	Reactome
Nonsense-Mediated Decay (NMD)	IEA	Reactome
rRNA processing	IEA	Reactome
rRNA processing in the nucleus and cytosol	IEA	Reactome
SRP-dependent cotranslational protein targeting to membrane	IEA	Reactome
Translation	IEA	Reactome

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