 Experiment description of studies that identified Pkm in sEVs |
| 1 |
| Experiment ID |
26 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Alix|GAPDH|HSP70|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
19109410
|
| Organism |
Mus musculus |
| Experiment description |
Proteomic and immunologic analyses of brain tumor exosomes. |
| Authors |
"Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD" |
| Journal name |
FASEB
|
| Publication year |
2008 |
| Sample |
Brain cancer cells |
| Sample name |
SMA560vIII |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.15 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [MALDI TOF/TOF]
Western blotting |
|
|
| 2 |
| Experiment ID |
907 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
|
Enriched markers |
|
✔
HSPA5
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
37309723
|
| Organism |
Mus musculus |
| Experiment description |
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells |
| Authors |
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Colon cancer cells |
| Sample name |
C26 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 3 |
| Experiment ID |
210 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23260141
|
| Organism |
Mus musculus |
| Experiment description |
Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration. |
| Authors |
"Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL." |
| Journal name |
Cell
|
| Publication year |
2012 |
| Sample |
Fibroblasts |
| Sample name |
Normal-Fibroblasts (L cells) |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 4 |
| Experiment ID |
214 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✘
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23658846
|
| Organism |
Mus musculus |
| Experiment description |
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. |
| Authors |
"Hassani K, Olivier M." |
| Journal name |
PLoS Negl Trop Dis
|
| Publication year |
2013 |
| Sample |
Macrophages |
| Sample name |
Leishmania-infected-Macrophage (J774A.1) |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation |
| Flotation density |
1.13-1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 5 |
| Experiment ID |
215 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✘
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23658846
|
| Organism |
Mus musculus |
| Experiment description |
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. |
| Authors |
"Hassani K, Olivier M." |
| Journal name |
PLoS Negl Trop Dis
|
| Publication year |
2013 |
| Sample |
Macrophages |
| Sample name |
LPS-treated-Macrophage (J774A.1) |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation |
| Flotation density |
1.13-1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 6 |
| Experiment ID |
216 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✘
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23658846
|
| Organism |
Mus musculus |
| Experiment description |
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. |
| Authors |
"Hassani K, Olivier M." |
| Journal name |
PLoS Negl Trop Dis
|
| Publication year |
2013 |
| Sample |
Macrophages |
| Sample name |
Normal-Macrophage (J774A.1) |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation |
| Flotation density |
1.13-1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 7 |
| Experiment ID |
15 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
17486113
|
| Organism |
Mus musculus
Homo sapiens |
| Experiment description |
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. |
| Authors |
"Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO" |
| Journal name |
NCB
|
| Publication year |
2007 |
| Sample |
Mast cells |
| Sample name |
MC9
Bone marrow-derived mast cells
HMC-1 |
| Isolation/purification methods |
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.11-1.21 g/mL
|
| Molecules identified in the study |
Protein
mRNA
miRNA |
| Methods used in the study |
Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array |
|
|
| 8 |
| Experiment ID |
14 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
|
Enriched markers |
|
✔
DNM
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
16081791
|
| Organism |
Mus musculus |
| Experiment description |
Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism. |
| Authors |
"Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L" |
| Journal name |
JIMMU
|
| Publication year |
2005 |
| Sample |
Microglia |
| Sample name |
N9 |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
1.15 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [LCQ DECA XP]
Western blotting |
|
|
| 9 |
| Experiment ID |
33 |
| MISEV standards |
|
✔
EM|IEM
|
Biophysical techniques |
|
✔
Alix|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
21136642
|
| Organism |
Mus musculus |
| Experiment description |
Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons? |
| Authors |
"Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J" |
| Journal name |
PROTEOMICS_CL
|
| Publication year |
2007 |
| Sample |
Oligodendrocytes |
| Sample name |
Oligodendrocytes
Oli-neu |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
1.10-1.14 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy |
|
|
| 10 |
| Experiment ID |
188 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
GAPDH|UCHL1|HSP90
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
19351151
|
| Organism |
Mus musculus |
| Experiment description |
Characterization of vesicles secreted from insulinoma NIT-1 cells. |
| Authors |
"Lee HS, Jeong J, Lee KJ." |
| Journal name |
J Proteome Res
|
| Publication year |
2009 |
| Sample |
Pancreatic cells |
| Sample name |
Pancreatic beta cell (NIT-1) |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 11 |
| Experiment ID |
908 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
|
Enriched markers |
|
✔
HSPA5
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
37309723
|
| Organism |
Mus musculus |
| Experiment description |
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells |
| Authors |
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
T-cell lymphoma cells |
| Sample name |
EL4 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
