Gene description for Usp25
Gene name ubiquitin specific peptidase 25
Gene symbol Usp25
Other names/aliases -
Species Mus musculus
 Database cross references - Usp25
ExoCarta ExoCarta_30940
Entrez Gene 30940
UniProt P57080  
 Usp25 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Usp25
Molecular Function
    cysteine-type endopeptidase activity GO:0004197 IBA
    cysteine-type peptidase activity GO:0008234 IEA
    ubiquitin-specific protease activity GO:0004843 ISS
    ubiquitinyl hydrolase activity GO:0036459 IEA
    SUMO binding GO:0032183 ISO
    peptidase activity GO:0008233 IEA
    hydrolase activity GO:0016787 IEA
Biological Process
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    protein K63-linked deubiquitination GO:0070536 ISO
    proteolysis GO:0006508 IEA
    protein deubiquitination GO:0016579 IBA
    regulation of proteasomal protein catabolic process GO:0061136 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 ISS
    protein K48-linked deubiquitination GO:0071108 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    proteasome complex GO:0000502 ISS
    nucleus GO:0005634 IBA
 Experiment description of studies that identified Usp25 in exosomes
1
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Usp25
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Usp25 is involved
No pathways found





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