Gene description for Cltc
Gene name clathrin, heavy polypeptide (Hc)
Gene symbol Cltc
Other names/aliases 3110065L21Rik
CHC
R74732
Species Mus musculus
 Database cross references - Cltc
ExoCarta ExoCarta_67300
Entrez Gene 67300
UniProt Q68FD5  
 Cltc identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Oligodendrocytes 21136642    
 Gene ontology annotations for Cltc
Molecular Function
    clathrin light chain binding GO:0032051 ISO
    heat shock protein binding GO:0031072 ISO
    poly(A) RNA binding GO:0044822 ISO
    structural molecule activity GO:0005198 IEA
    double-stranded RNA binding GO:0003725 ISO
    peptide binding GO:0042277 ISO
    protein binding GO:0005515 IPI
    ankyrin binding GO:0030506 ISO
    protein C-terminus binding GO:0008022 ISO
    protein kinase binding GO:0019901 IPI
Biological Process
    negative regulation of protein localization to plasma membrane GO:1903077 ISO
    vesicle-mediated transport GO:0016192 IEA
    negative regulation of hyaluronan biosynthetic process GO:1900126 ISO
    Golgi organization GO:0007030 ISO
    receptor-mediated endocytosis GO:0006898 ISO
    intracellular protein transport GO:0006886 IEA
    receptor internalization GO:0031623 ISO
    mitotic nuclear division GO:0007067 ISO
    mitotic spindle assembly GO:0090307 ISO
    osteoblast differentiation GO:0001649 ISO
    transferrin transport GO:0033572 ISO
Subcellular Localization
    coated pit GO:0005905 IEA
    spindle GO:0005819 ISO
    clathrin complex GO:0071439 ISO
    cytoplasm GO:0005737 ISO
    cytoplasmic vesicle GO:0031410 IEA
    membrane GO:0016020 ISO
    Myb complex GO:0031523 ISO
    sarcolemma GO:0042383 ISO
    terminal bouton GO:0043195 ISO
    extracellular vesicle GO:1903561 ISO
    T-tubule GO:0030315 ISO
    membrane coat GO:0030117 IDA
    protein complex GO:0043234 ISO
    clathrin coat of coated pit GO:0030132 IEA
    clathrin coat GO:0030118 ISO
    clathrin-coated vesicle GO:0030136 ISO
    myelin sheath GO:0043209 IDA
    mitochondrion GO:0005739 IDA
    clathrin coat of trans-Golgi network vesicle GO:0030130 IEA
    extracellular exosome GO:0070062 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    focal adhesion GO:0005925 ISO
 Experiment description of studies that identified Cltc in exosomes
1
Experiment ID 210
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 214
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 33
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Cltc
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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