Gene description for Cltc
Gene name clathrin, heavy polypeptide (Hc)
Gene symbol Cltc
Other names/aliases 3110065L21Rik
CHC
R74732
Species Mus musculus
 Database cross references - Cltc
ExoCarta ExoCarta_67300
Vesiclepedia VP_67300
Entrez Gene 67300
UniProt Q68FD5  
 Cltc identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
Oligodendrocytes 21136642    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cltc
Molecular Function
    double-stranded RNA binding GO:0003725 ISO
    structural molecule activity GO:0005198 IEA
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
    ankyrin binding GO:0030506 ISO
    heat shock protein binding GO:0031072 ISO
    clathrin light chain binding GO:0032051 IBA
    clathrin light chain binding GO:0032051 IEA
    clathrin light chain binding GO:0032051 ISO
    peptide binding GO:0042277 ISO
    low-density lipoprotein particle receptor binding GO:0050750 ISO
    disordered domain specific binding GO:0097718 ISO
    protein serine/threonine kinase binding GO:0120283 ISO
    ubiquitin-specific protease binding GO:1990381 ISO
Biological Process
    mitotic cell cycle GO:0000278 IBA
    mitotic cell cycle GO:0000278 ISO
    intracellular protein transport GO:0006886 IEA
    receptor-mediated endocytosis GO:0006898 IBA
    receptor-mediated endocytosis GO:0006898 ISO
    autophagy GO:0006914 IEA
    Golgi organization GO:0007030 ISO
    receptor internalization GO:0031623 ISO
    transferrin transport GO:0033572 ISO
    retrograde transport, endosome to Golgi GO:0042147 ISO
    clathrin coat assembly GO:0048268 IBA
    clathrin coat assembly GO:0048268 ISO
    synaptic vesicle endocytosis GO:0048488 ISO
    cell division GO:0051301 IEA
    regulation of mitotic spindle organization GO:0060236 ISO
    clathrin coat disassembly GO:0072318 ISS
    clathrin-dependent endocytosis GO:0072583 ISO
    mitotic spindle assembly GO:0090307 ISO
    amyloid-beta clearance by transcytosis GO:0150093 ISO
    negative regulation of hyaluronan biosynthetic process GO:1900126 ISO
    negative regulation of hyaluronan biosynthetic process GO:1900126 ISS
    negative regulation of protein localization to plasma membrane GO:1903077 ISO
Subcellular Localization
    mitochondrion GO:0005739 HDA
    lysosome GO:0005764 ISO
    endosome GO:0005768 ISO
    spindle GO:0005819 IBA
    spindle GO:0005819 IEA
    spindle GO:0005819 ISO
    clathrin-coated pit GO:0005905 IDA
    membrane GO:0016020 IMP
    membrane coat GO:0030117 IDA
    clathrin coat GO:0030118 ISO
    clathrin coat of trans-Golgi network vesicle GO:0030130 IEA
    clathrin coat of coated pit GO:0030132 IEA
    clathrin-coated vesicle GO:0030136 ISO
    T-tubule GO:0030315 ISO
    Myb complex GO:0031523 ISO
    protein-containing complex GO:0032991 ISO
    sarcolemma GO:0042383 ISO
    melanosome GO:0042470 IEA
    myelin sheath GO:0043209 HDA
    clathrin-coated endocytic vesicle GO:0045334 IBA
    clathrin complex GO:0071439 IBA
    clathrin complex GO:0071439 IEA
    clathrin complex GO:0071439 ISO
    mitotic spindle GO:0072686 ISO
    photoreceptor ribbon synapse GO:0098684 IDA
    presynaptic endocytic zone membrane GO:0098835 IDA
    postsynaptic endocytic zone GO:0098843 EXP
    postsynaptic endocytic zone GO:0098843 IDA
    extrinsic component of synaptic vesicle membrane GO:0098850 ISO
    glutamatergic synapse GO:0098978 EXP
    glutamatergic synapse GO:0098978 IDA
    mitotic spindle microtubule GO:1990498 ISO
 Experiment description of studies that identified Cltc in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 1101
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 1102
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
8
Experiment ID 1103
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
9
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
10
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cltc
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNCA 6622
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
2 Ap1b1 11764
Co-fractionation Mus musculus
3 Synj1 104015
Co-fractionation Mus musculus
4 Usp25 30940
Affinity Capture-MS Mus musculus
5 Tnk2  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
6 Tom1l1  
Affinity Capture-Western Mus musculus
7 Vps33a  
Co-fractionation Mus musculus
8 Spg20  
Co-fractionation Mus musculus
9 MTNR1B  
Co-purification Homo sapiens
10 Dnajc6  
Co-fractionation Mus musculus
11 Dnm1 13429
Affinity Capture-Western Mus musculus
12 Scyl2  
Co-fractionation Mus musculus
13 Kctd13  
Affinity Capture-MS Mus musculus
14 Dlg4  
Affinity Capture-MS Mus musculus
15 Pfn1 18643
Affinity Capture-MS Mus musculus
16 Atg16l1  
Affinity Capture-MS Mus musculus
17 Ywhaz 22631
Affinity Capture-MS Mus musculus
18 Mettl21e  
Affinity Capture-MS Mus musculus
19 AMPH 273
Reconstituted Complex Homo sapiens
20 Tmem173  
Proximity Label-MS Mus musculus
21 Grin2b  
Affinity Capture-Western Mus musculus
22 Agap2  
Affinity Capture-MS Mus musculus
23 Unk  
Affinity Capture-RNA Mus musculus
24 Hgs 15239
Affinity Capture-Western Mus musculus
25 Hip1r  
Co-fractionation Mus musculus
26 Grin1  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
27 TXNIP 10628
Affinity Capture-Western Homo sapiens
28 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
29 Eed  
Affinity Capture-MS Mus musculus
30 Atg5  
Affinity Capture-MS Mus musculus
31 Ywhae 22627
Affinity Capture-MS Mus musculus
32 Mapt  
Affinity Capture-MS Mus musculus
33 Tia1  
Affinity Capture-MS Mus musculus
34 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Cltc is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Axon guidance IEA Reactome
Beta-catenin independent WNT signaling IEA Reactome
Cargo recognition for clathrin-mediated endocytosis IEA Reactome
Clathrin-mediated endocytosis IEA Reactome
Developmental Biology IEA Reactome
Formation of annular gap junctions IEA Reactome
Gap junction degradation IEA Reactome
Gap junction trafficking IEA Reactome
Gap junction trafficking and regulation IEA Reactome
Golgi Associated Vesicle Biogenesis IEA Reactome
Immune System IEA Reactome
L1CAM interactions IEA Reactome
LDL clearance IEA Reactome
Lysosome Vesicle Biogenesis IEA Reactome
Membrane Trafficking IEA Reactome
MHC class II antigen presentation IEA Reactome
Nervous system development IEA Reactome
PCP/CE pathway IEA Reactome
Plasma lipoprotein assembly, remodeling, and clearance IEA Reactome
Plasma lipoprotein clearance IEA Reactome
Recycling pathway of L1 IEA Reactome
Retrograde neurotrophin signalling IEA Reactome
RHO GTPase cycle IEA Reactome
RHOU GTPase cycle IEA Reactome
RHOV GTPase cycle IEA Reactome
Signal Transduction IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by WNT IEA Reactome
trans-Golgi Network Vesicle Budding IEA Reactome
Transport of small molecules IEA Reactome
Vesicle-mediated transport IEA Reactome
VLDLR internalisation and degradation IEA Reactome
WNT5A-dependent internalization of FZD2, FZD5 and ROR2 IEA Reactome
WNT5A-dependent internalization of FZD4 IEA Reactome





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