Gene description for KCNH1
Gene name potassium channel, voltage gated eag related subfamily H, member 1
Gene symbol KCNH1
Other names/aliases EAG
EAG1
Kv10.1
TMBTS
h-eag
Species Homo sapiens
 Database cross references - KCNH1
ExoCarta ExoCarta_3756
Vesiclepedia VP_3756
Entrez Gene 3756
HGNC 6250
MIM 603305
UniProt O95259  
 KCNH1 identified in sEVs derived from the following tissue/cell type
Cholangiocarcinoma cells 31054213    
Cholangiocarcinoma cells 31054213    
 Gene ontology annotations for KCNH1
Molecular Function
    delayed rectifier potassium channel activity GO:0005251 IBA
    delayed rectifier potassium channel activity GO:0005251 IDA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
    cyclic nucleotide binding GO:0030551 ISS
    phosphatidylinositol bisphosphate binding GO:1902936 IDA
Biological Process
    potassium ion transport GO:0006813 IDA
    myoblast fusion GO:0007520 TAS
    regulation of cell population proliferation GO:0042127 IMP
    regulation of membrane potential GO:0042391 IBA
    cellular response to calcium ion GO:0071277 IMP
    potassium ion transmembrane transport GO:0071805 IBA
    potassium ion transmembrane transport GO:0071805 IDA
Subcellular Localization
    nuclear inner membrane GO:0005637 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IMP
    plasma membrane GO:0005886 TAS
    voltage-gated potassium channel complex GO:0008076 IBA
    voltage-gated potassium channel complex GO:0008076 IDA
    axon GO:0030424 IEA
    dendrite GO:0030425 IEA
    early endosome membrane GO:0031901 IDA
    presynaptic membrane GO:0042734 IEA
    perikaryon GO:0043204 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    postsynaptic density membrane GO:0098839 IEA
 Experiment description of studies that identified KCNH1 in sEVs
1
Experiment ID 1223
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 31054213    
Organism Homo sapiens
Experiment description Phosphoproteome Profiling of Isogenic Cancer Cell-Derived Exosome Reveals HSP90 as a Potential Marker for Human Cholangiocarcinoma
Authors "Weeraphan C, Phongdara A, Chaiyawat P, Diskul-Na-Ayudthaya P, Chokchaichamnankit D, Verathamjamras C, Netsirisawan P, Yingchutrakul Y, Roytrakul S, Champattanachai V, Svasti J, Srisomsap C."
Journal name Proteomics
Publication year 2019
Sample Cholangiocarcinoma cells
Sample name KKU-M213 - in-gel digestion
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 1224
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 31054213    
Organism Homo sapiens
Experiment description Phosphoproteome Profiling of Isogenic Cancer Cell-Derived Exosome Reveals HSP90 as a Potential Marker for Human Cholangiocarcinoma
Authors "Weeraphan C, Phongdara A, Chaiyawat P, Diskul-Na-Ayudthaya P, Chokchaichamnankit D, Verathamjamras C, Netsirisawan P, Yingchutrakul Y, Roytrakul S, Champattanachai V, Svasti J, Srisomsap C."
Journal name Proteomics
Publication year 2019
Sample Cholangiocarcinoma cells
Sample name KKU-M213D5 - in-gel digestion
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KCNH1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KCNC1  
Two-hybrid Homo sapiens
2 MKRN1 23608
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
3 SDCBP 6386
Two-hybrid Homo sapiens
4 FASTKD5  
Two-hybrid Homo sapiens
5 WDYHV1  
Two-hybrid Homo sapiens
6 CALM1 801
Reconstituted Complex Homo sapiens
7 KCNB1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
8 KCNF1  
Two-hybrid Homo sapiens
9 METTL3  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which KCNH1 is involved
PathwayEvidenceSource
Neuronal System TAS Reactome
Potassium Channels TAS Reactome
Voltage gated Potassium channels TAS Reactome





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