Gene description for Pabpn1
Gene name poly(A) binding protein, nuclear 1
Gene symbol Pabpn1
Other names/aliases PAB2
Pabp3
mPABII
Species Mus musculus
 Database cross references - Pabpn1
ExoCarta ExoCarta_54196
Vesiclepedia VP_54196
Entrez Gene 54196
UniProt Q8CCS6  
 Pabpn1 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Pabpn1
Molecular Function
    protein binding GO:0005515 IPI
    poly(A) binding GO:0008143 IBA
    poly(A) binding GO:0008143 TAS
    identical protein binding GO:0042802 ISO
    RNA polymerase binding GO:0070063 IDA
Biological Process
    MAPK cascade GO:0000165 IMP
    mRNA processing GO:0006397 IEA
    poly(A)+ mRNA export from nucleus GO:0016973 ISO
    regulation of mRNA 3'-end processing GO:0031440 TAS
    cellular response to lipopolysaccharide GO:0071222 IMP
    positive regulation of polynucleotide adenylyltransferase activity GO:1904247 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytosol GO:0005829 TAS
    nuclear speck GO:0016607 IEA
    nuclear inclusion body GO:0042405 ISO
    nuclear inclusion body GO:0042405 ISS
    ribonucleoprotein complex GO:1990904 IDA
    ribonucleoprotein complex GO:1990904 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Pabpn1 in exosomes
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Pabpn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ago2 239528
Co-fractionation Mus musculus
2 Rplp0 11837
Co-fractionation Mus musculus
3 Ncbp1 433702
Co-fractionation Mus musculus
4 Tardbp 230908
Co-fractionation Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 MKRN1 23608
Affinity Capture-MS Homo sapiens
7 Tfe3  
Affinity Capture-MS Mus musculus
8 Atg16l1  
Affinity Capture-MS Mus musculus
9 Ilf2 67781
Co-fractionation Mus musculus
10 Syncrip 56403
Co-fractionation Mus musculus
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