Gene description for Ago2
Gene name argonaute RISC catalytic subunit 2
Gene symbol Ago2
Other names/aliases 1110029L17Rik
2310051F07Rik
AI225898
AL022874
AW546247
ENSMUSG00000072493
Eif2c2
Gerp95
Gm10365
mKIAA4215
Species Mus musculus
 Database cross references - Ago2
ExoCarta ExoCarta_239528
Entrez Gene 239528
UniProt Q8CJG0  
 Ago2 identified in exosomes derived from the following tissue/cell type
Neural stem cells 25242146    
 Gene ontology annotations for Ago2
Molecular Function
    RNA 7-methylguanosine cap binding GO:0000340 ISO
    metal ion binding GO:0046872 IEA
    RNA polymerase II core binding GO:0000993 ISO
    endoribonuclease activity GO:0004521 ISO
    endoribonuclease activity, cleaving miRNA-paired mRNA GO:0090624 ISO
    single-stranded RNA binding GO:0003727 ISO
    pre-miRNA binding GO:0070883 ISO
    nucleic acid binding GO:0003676 IEA
    RNA binding GO:0003723 IDA
    endonuclease activity GO:0004519 IEA
    protein binding GO:0005515 IPI
    double-stranded RNA binding GO:0003725 ISO
    core promoter binding GO:0001047 ISO
    nuclease activity GO:0004518 IEA
    miRNA binding GO:0035198 ISO
    endoribonuclease activity, cleaving siRNA-paired mRNA GO:0070551 ISO
    hydrolase activity GO:0016787 IEA
    mRNA binding GO:0003729 IDA
    poly(A) RNA binding GO:0044822 ISO
    siRNA binding GO:0035197 ISO
Biological Process
    siRNA loading onto RISC involved in RNA interference GO:0035087 ISO
    positive regulation of nuclear-transcribed mRNA poly(A) tail shortening GO:0060213 IMP
    RNA secondary structure unwinding GO:0010501 ISO
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:1900153 IMP
    negative regulation of translation involved in gene silencing by miRNA GO:0035278 ISO
    mRNA cleavage involved in gene silencing by miRNA GO:0035279 ISO
    positive regulation of gene expression GO:0010628 IMP
    gene silencing by RNA GO:0031047 IMP
    pre-miRNA processing GO:0031054 ISO
    miRNA loading onto RISC involved in gene silencing by miRNA GO:0035280 ISO
    cell differentiation GO:0030154 IEA
    mRNA cleavage GO:0006379 IMP
    negative regulation of translational initiation GO:0045947 ISO
    mRNA cleavage involved in gene silencing by siRNA GO:0090625 ISO
    post-embryonic development GO:0009791 IMP
    regulation of translation GO:0006417 IEA
    regulation of transcription, DNA-templated GO:0006355 IEA
    multicellular organismal development GO:0007275 IEA
    transcription, DNA-templated GO:0006351 IEA
    production of miRNAs involved in gene silencing by miRNA GO:0035196 ISO
    miRNA metabolic process GO:0010586 IDA
    RNA phosphodiester bond hydrolysis, endonucleolytic GO:0090502 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    nucleus GO:0005634 IDA
    dendrite GO:0030425 IDA
    RISC complex GO:0016442 ISO
    cytosol GO:0005829 IDA
    ribonucleoprotein complex GO:0030529 ISO
    RISC-loading complex GO:0070578 ISO
    mRNA cap binding complex GO:0005845 ISO
    cytoplasmic mRNA processing body GO:0000932 ISO
    membrane GO:0016020 ISO
    micro-ribonucleoprotein complex GO:0035068 ISO
    polysome GO:0005844 ISO
 Experiment description of studies that identified Ago2 in exosomes
1
Experiment ID 262
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70|HSP90
EV Cytosolic markers
CD63|CD9|
EV Membrane markers
EV Negative markers
Y
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Microarray
 Protein-protein interactions for Ago2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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