Gene description for Eprs
Gene name glutamyl-prolyl-tRNA synthetase
Gene symbol Eprs
Other names/aliases 2410081F06Rik
3010002K18Rik
C79379
Qprs
Species Mus musculus
 Database cross references - Eprs
ExoCarta ExoCarta_107508
Entrez Gene 107508
UniProt Q8CGC7  
 Eprs identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Pancreatic cells 19351151    
 Gene ontology annotations for Eprs
Molecular Function
    glutamate-tRNA ligase activity GO:0004818 IEA
    ligase activity, forming aminoacyl-tRNA and related compounds GO:0016876 IEA
    ATP binding GO:0005524 IEA
    ligase activity GO:0016874 IEA
    RNA stem-loop binding GO:0035613 ISO
    proline-tRNA ligase activity GO:0004827 IEA
    catalytic activity GO:0003824 IEA
    nucleotide binding GO:0000166 IEA
    aminoacyl-tRNA ligase activity GO:0004812 IEA
    RNA binding GO:0003723 IEA
    GTPase binding GO:0051020 ISO
Biological Process
    cellular response to interferon-gamma GO:0071346 ISO
    regulation of translation GO:0006417 IEA
    negative regulation of translation GO:0017148 ISO
    metabolic process GO:0008152 IEA
    glutamyl-tRNA aminoacylation GO:0006424 IEA
    prolyl-tRNA aminoacylation GO:0006433 IEA
    translation GO:0006412 IEA
    tRNA aminoacylation for protein translation GO:0006418 IEA
    tRNA aminoacylation GO:0043039 IEA
Subcellular Localization
    membrane GO:0016020 ISO
    GAIT complex GO:0097452 IDA
    aminoacyl-tRNA synthetase multienzyme complex GO:0017101 IDA
    ribonucleoprotein complex GO:0030529 ISO
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified Eprs in exosomes
1
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Eprs
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Eprs is involved
No pathways found





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