Gene description for CDC42BPA
Gene name CDC42 binding protein kinase alpha (DMPK-like)
Gene symbol CDC42BPA
Other names/aliases MRCK
MRCKA
PK428
Species Homo sapiens
 Database cross references - CDC42BPA
ExoCarta ExoCarta_8476
Vesiclepedia VP_8476
Entrez Gene 8476
HGNC 1737
MIM 603412
UniProt Q5VT25  
 CDC42BPA identified in exosomes derived from the following tissue/cell type
Bladder cancer cells 20224111    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Urine 19056867    
 Gene ontology annotations for CDC42BPA
Molecular Function
    magnesium ion binding GO:0000287 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    identical protein binding GO:0042802 IPI
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    cell migration GO:0016477 IMP
    actin cytoskeleton organization GO:0030036 IDA
    actomyosin structure organization GO:0031032 IBA
    actomyosin structure organization GO:0031032 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IBA
    cell-cell junction GO:0005911 ISS
    lamellipodium GO:0030027 ISS
    cell leading edge GO:0031252 ISS
    actomyosin GO:0042641 IBA
    actomyosin GO:0042641 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified CDC42BPA in exosomes
1
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
8
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for CDC42BPA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 ADRBK1 156
Affinity Capture-MS Homo sapiens
3 GJD3  
Proximity Label-MS Homo sapiens
4 LGR4 55366
Affinity Capture-MS Homo sapiens
5 OCLN 100506658
Proximity Label-MS Homo sapiens
6 FAM167A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 MARCKS 4082
Proximity Label-MS Homo sapiens
8 GJA1 2697
Proximity Label-MS Homo sapiens
9 PARD3 56288
Proximity Label-MS Homo sapiens
10 LAMP2 3920
Proximity Label-MS Homo sapiens
11 STX4 6810
Proximity Label-MS Homo sapiens
12 SOX2  
Affinity Capture-MS Homo sapiens
13 SEPT9 10801
Proximity Label-MS Homo sapiens
14 SP1  
Affinity Capture-MS Homo sapiens
15 MLLT4 4301
Proximity Label-MS Homo sapiens
16 CTDSPL2  
Affinity Capture-MS Homo sapiens
17 STX7 8417
Proximity Label-MS Homo sapiens
18 CDC42BPB 9578
Affinity Capture-MS Homo sapiens
19 Ttll7  
Affinity Capture-MS Mus musculus
20 Fam60a 56306
Affinity Capture-MS Mus musculus
21 GPRC5B 51704
Affinity Capture-MS Homo sapiens
22 YWHAG 7532
Affinity Capture-MS Homo sapiens
23 SLC31A1 1317
Affinity Capture-MS Homo sapiens
24 LURAP1L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 PPM1A 5494
Biochemical Activity Homo sapiens
26 STX6 10228
Proximity Label-MS Homo sapiens
27 DNAJC7 7266
Proximity Label-MS Homo sapiens
28 FCGR1A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 RAB35 11021
Proximity Label-MS Homo sapiens
30 CSNK2B 1460
Affinity Capture-MS Homo sapiens
31 EBAG9 9166
Proximity Label-MS Homo sapiens
32 RBM8A 9939
Affinity Capture-MS Homo sapiens
33 LAMTOR1 55004
Proximity Label-MS Homo sapiens
34 TGM2 7052
Affinity Capture-MS Homo sapiens
35 CXADR 1525
Proximity Label-MS Homo sapiens
36 LYN 4067
Proximity Label-MS Homo sapiens
37 MCAM 4162
Proximity Label-MS Homo sapiens
38 PRPS2 5634
Affinity Capture-MS Homo sapiens
39 DNAJC5 80331
Proximity Label-MS Homo sapiens
40 Ctnnbl1  
Affinity Capture-MS Mus musculus
41 YWHAE 7531
Affinity Capture-MS Homo sapiens
42 DIRAS3  
Proximity Label-MS Homo sapiens
43 NTRK1 4914
Affinity Capture-MS Homo sapiens
44 RAB7A 7879
Proximity Label-MS Homo sapiens
45 CENPQ  
Affinity Capture-MS Homo sapiens
46 Arhgef2  
Affinity Capture-MS Mus musculus
47 KIF20B  
Affinity Capture-MS Homo sapiens
48 MLNR  
Affinity Capture-MS Homo sapiens
49 YWHAH 7533
Affinity Capture-MS Homo sapiens
50 PTPRM 5797
Proximity Label-MS Homo sapiens
51 BCL2L12 83596
Affinity Capture-MS Homo sapiens
52 CDK9 1025
Affinity Capture-MS Homo sapiens
53 EPB41L4A 64097
Proximity Label-MS Homo sapiens
54 LAMP1 3916
Proximity Label-MS Homo sapiens
55 EPPK1 83481
Affinity Capture-MS Homo sapiens
56 YWHAZ 7534
Affinity Capture-MS Homo sapiens
57 MECP2 4204
Affinity Capture-MS Homo sapiens
58 Rabep2  
Affinity Capture-MS Mus musculus
59 ARF6 382
Proximity Label-MS Homo sapiens
60 CDC42 998
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
61 MYC  
Affinity Capture-MS Homo sapiens
62 INO80B 83444
Affinity Capture-MS Homo sapiens
63 RHOB 388
Proximity Label-MS Homo sapiens
64 KRAS 3845
Proximity Label-MS Homo sapiens
65 CDKN2D  
Affinity Capture-MS Homo sapiens
66 FLOT1 10211
Proximity Label-MS Homo sapiens
67 Sart1  
Affinity Capture-MS Mus musculus
68 KRT18 3875
Affinity Capture-MS Homo sapiens
69 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
70 Ruvbl2 20174
Affinity Capture-MS Mus musculus
71 EZR 7430
Proximity Label-MS Homo sapiens
72 HAVCR2  
Affinity Capture-MS Homo sapiens
73 Spire2  
Affinity Capture-MS Mus musculus
74 LCK 3932
Proximity Label-MS Homo sapiens
75 SEPT10 151011
Proximity Label-MS Homo sapiens
76 HDAC1 3065
Affinity Capture-MS Homo sapiens
77 C11orf52 91894
Proximity Label-MS Homo sapiens
78 RAB2A 5862
Proximity Label-MS Homo sapiens
79 DNAJC5B  
Proximity Label-MS Homo sapiens
80 PYGB 5834
Co-fractionation Homo sapiens
81 RPL7A 6130
Affinity Capture-MS Homo sapiens
82 RAB11A 8766
Proximity Label-MS Homo sapiens
83 RPL28 6158
Affinity Capture-MS Homo sapiens
84 KCTD17 79734
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 PTGES3 10728
Affinity Capture-MS Homo sapiens
86 SKA3  
Affinity Capture-MS Homo sapiens
87 FAM212B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 AR 367
Affinity Capture-MS Homo sapiens
89 NEFM 4741
Affinity Capture-MS Homo sapiens
90 CAV1 857
Proximity Label-MS Homo sapiens
91 LURAP1  
Affinity Capture-MS Homo sapiens
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